T 2 toxin

The β-glucan enzymatic assay kit (β-glucan assay kit, yeast and mushroom) was purchased from Megazyme Ltd. (Wicklow, Ireland). The mycotoxin test kit (RIDASCREEN^®FAST mycotoxin test kit; RIDASCREEN^®FAST Aflatoxin B1, Ochratoxin A, T-2 Toxin, Zearalenone and DON) and standard mycotoxins (aflatoxin B1, AFB1; T-2 Toxin, T-2; Ochratoxin A, OTA; zearalenone, ZEA and deoxynivalenol, DON) were purchased from R-Biopharm AG (Darmstadt, Germany). The commercial mycotoxin binder (Toxibond^®Pro) was purchased from Biomix Co., Ltd. (Sabaneta, Colombia), and yeast extract was purchased from Himedia (Mumbai, India). Malt extract and peptone (Bacto™ Peptone) were purchased from BD Difco™ (Franklin Lakes, NJ, USA). Commercial-grade maltodextrin was purchased from Union Science Co., Ltd. (Chiang Mai, Thailand). Glass beads (3 mm in diameter) were purchased from Paul Marienfeld GmbH & Co., Ltd. KG (Lauda-Königshofen, Germany). All other chemicals used in this study were of analytical grade.

For the preparation of samples, o-phthaldialdehyde (≥97% for HPLC), 2-mercaptoethanol (≥99.0%), potassium bromide (Purum p.a., ≥99.5%) and boron trifluoride BF3 (10–20% in methanol) were from Sigma-Aldrich (Saint-Quentin Fallavier, France). Purified water (18 MΩ cm) was produced in-house from an Academic MilliQ model water purification unit (Millipore, EMD Millipore Corporation, Billerica, MA, USA). For the preparation of LC mobile phases, acetonitrile (RS for isocratic HPLC, Carlo-Erba Reagents, Val-de-Reuil, France) and methanol (Lichrosolv for HPLC, Merck, Darmstadt, Germany) were used. All other solvents and reagents were of analytical grade.
The mycotoxin primary standard solution of OTA (10 µg/mL in acetonitrile) was from Sigma-Aldrich Chemie (Schnelldorf, Germany). FB1 and FB2 together (50 µg/mL each in an acetonitrile–water mixture) were from Romer labs (Getzersdorf, Austria). Aflatoxins B1, B2, G1 and G2 (250 ng/mL of each in acetonitrile) were purchased from Libios (Bully, France) and T-2 Toxin (100 µg/mL in acetonitrile), HT-2 Toxin (100 µg/mL in acetonitrile), DON (100 µg/mL in methanol) and ZEA (25 µg/mL in methanol) were procured from R-Biopharm (Saint-Didier-au-Mont-d’Or, France).