After deparaffinization and blocking, sections of CRC specimen were incubated with primary anti-Oct4 rabbit polyclonal antibody (#2570; Cell Signaling Technology Inc., Beverly, MA, USA) at a dilution of 1 : 200 overnight at 4°C. Vectastain Universal Elite (Vector Laboratories, Burlingame, CA, USA) was used to detect the signal. Diaminobenzidine was used for color modification. All sections were counterstained with hematoxylin.
Vectastain universal elite
Manufactured by Vector Laboratories
Sourced in United States
The Vectastain Universal Elite is a reagent kit used for the detection of target antigens in immunohistochemistry and immunocytochemistry applications. It contains the necessary components to perform a sensitive avidin-biotin-peroxidase complex (ABC) detection method, which amplifies the signal for improved visualization of the target.
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Lab products found in correlation
2 protocols using vectastain universal elite
1
Immunohistochemical Detection of Oct4 in CRC
2
Immunohistochemical Detection of HNF1A
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After deparaffinization and blocking, the sections were incubated with primary anti-HNF1A rabbit polyclonal antibody (ab204306; Abcam, Cambridge, UK) at a dilution of 1:200 overnight at 4 °C. The sections were incubated with biotinylated secondary antibody (Vectastain Universal Elite; Vector Laboratories, Burlingame, CA, USA) for 30 min, and signals were detected using the ImmPACT DAB kit (Vector Laboratories) with 5-min incubation. All sections were counterstained with hematoxylin.
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