Cyquant nf assay kit

Manufactured by Thermo Fisher Scientific

The CyQuant™NF assay kit is a fluorescence-based method for quantifying the number of cells in a sample. It provides a sensitive and simple way to measure cell proliferation and cytotoxicity. The kit uses a proprietary dye that binds to cellular nucleic acids, enabling the determination of cell number through fluorescence measurement.

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Synergy ht, by Agilent Technologies (1 mentions)

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CyQUANT NF Cell Proliferation Assay Kit (135 citations) CyQUANT (133 citations) CyQUANT assay (96 citations) CyQuant kit (59 citations) CyQUANT assay kit (26 citations) CyQUANT® NF assay (15 citations) CyQuant NF (10 citations) CyQuant NF kit (7 citations) CyQuant NF proliferation assay kit (5 citations)

2 protocols using cyquant nf assay kit

Quantifying SB216763 Effects on Endothelial Cell Proliferation

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Cells between passage 3 and 4 were seeded into wells of a 96 well plate at a density of 1000 cells per well and allowed to incubate in 100 μL of full culture media for 24 hours. The culture media was then replaced with microvascular endothelial cell basal media without growth supplements overnight. Following starvation, cells were treated with basal media supplemented with 1% FBS and either DMSO (vehicle) or SB216763. The optimal dosing of SB216763 after dose titration experiments was 1μM. Cell proliferation was determined using CyQuant^™NF assay kit (Invitrogen, Carlsbad, CA) per the manufacturer instructions. Fluorescence intensity was determined using a Biotek Synergy HT microplate reader (Biotek, Winooski, VT).

Stump B., Shrestha S., Lamattina A.M., Louis P.H., Cho W., Perrella M.A., Ai X., Rosas I.O., Wagner F.F., Priolo C., Astin J, & El-Chemaly S. (2019). Glycogen synthase kinase 3-β inhibition induces lymphangiogenesis through β-catenin-dependent and mTOR-independent pathways. PLoS ONE, 14(4), e0213831.

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H2S Fluorescent Probe Quantification

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H₂S fluorescent probe, P3 (Sigma-Aldrich/Millipore; 534329)^{43 (link)} was used at a final concentration of 10 μM (optimal concentration empirically determined). Cells were incubated with the probe for 30 minutes prior to fluorescence measurements using a Flexstation plate reader (Ex/Em: 375/505 nm). Measurements were normalized to cell number, which was quantified using CyQUANT NF assay kit (Invitrogen) following manufacturer’s instructions (Ex/Em: 480/520) (cell quantification was done in wells receiving the vehicle control instead of P3).

Bartman C.M., Schiliro M., Helan M., Prakash Y.S., Linden D, & Pabelick C. (2020). Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9), 12991-13004.

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