Non essential amino acid solution neaa

WJ-MSCs (seeding density: 5×10⁶ cells per scaffold) were loaded into the pores of PLGA scaffolds in a growth medium containing α-modified Minimum Essential Medium Eagle with L-glutamine and sodium bicarbonate (α-MEM; Sigma-Aldrich) supplemented with 20 % fetal bovine serum (FBS; Life Technologies), penicillin (100 U/ml; Sigma Aldrich), streptomycin (100 μg/ml; Sigma Aldrich) and 1 % Non-Essential Amino Acid Solution (NEAA; Sigma Aldrich). To observe the effects of seeding, the cells were stained with CellLight Tubulin-GFP BacMan 2.0 (Life Technologies) reagent according to the manufacturer’s protocol. The reagent labels tubulin with green fluorescent protein (GFP) in live cells. The invasion of the scaffolds by WJ-MSCs was assessed at days 1, 7, and 14 of culture in the growth medium, using a confocal laser scanning microscope (Olympus FluoView).

(±)-α-tocopherol; methyl viologen dichloride hydrate; DMEM:F12 medium; penicillin–streptomycin solution hybri-max (P/S); fetal bovine serum (FBS); L-glutamine (L-Glut); non-essential amino acid solution (NEAA); trypan blue; AM281; WIN55,212-2; mesylate salt; 2-mercaptobenzothiazole; and 1,5-diaminophtalene were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Methanol and acetonitrile were purchased from Panreac (Barcelona, Spain) and cesium chloride was purchased from HoneyWell (Charlotte, NC, USA).