Dulbecco s modified eagle s medium dmem d5796

Manufactured by Merck Group

Sourced in Germany, United Kingdom, United States

Dulbecco's modified Eagle's medium (DMEM) (D5796) is a cell culture medium formulation developed by Dr. Harry Eagle. It is a widely used basal medium for the in vitro cultivation of many different cell types, including adherent and suspension cells. The core function of DMEM is to provide cells with the necessary nutrients, amino acids, vitamins, and other components to support their growth and proliferation in a controlled laboratory environment.

Automatically generated - may contain errors

Lab products found in correlation

α tubulin t9026, by Merck Group (1 mentions) Cd24 sc 11406, by Santa Cruz Biotechnology (1 mentions) Lc3 3868, by Cell Signaling Technology (1 mentions) Collagenase type 2 ls004176, by Worthington (1 mentions) Beclin 1 sc 11 427, by Santa Cruz Biotechnology (1 mentions) N6 2 o dibutyryladenosine 3 5 cyclic monophosphate sodium salt dbcamp, by Merck Group (1 mentions) 3 3 5 triiodo l thyronine sodium salt t3, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

3 protocols using dulbecco s modified eagle s medium dmem d5796

Detailed Autophagy and Apoptosis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies and apoptosis detection kit used were as follows: LC3 (3868), Cell Signaling Technology (Danvers, MA, USA); p62/SQSTM1 (ab56416) and Pax1 (ab95227), Abcam (Cambridge, UK); beclin1 (sc-11427), brachyury (sc-17743), and CD24 (sc-11406), Santa Cruz Biotechnology (Santa Cruz, CA, USA); α-tubulin (T9026), Sigma-Aldrich (St. Louis, MO, USA); TUNEL (11684795910), Roche Diagnostics (Mannheim, Germany).
The reagents used for cell culture were as follows: Dulbecco’s modified Eagle’s medium (DMEM) (D5796) and fetal bovine serum (FBS) (F2442), Sigma-Aldrich; penicillin/streptomycin (26253-84), Nakarai Tesque (Kyoto, Japan); collagenase type 2 (LS004176), Worthington Biochemical (Lakewood, NJ, USA).
The reagents used for Western blotting and immunostaining were obtained from Thermo Fisher Scientific (Waltham, MA, USA), Nakarai Tesque, Bio Craft (Tokyo, Japan), GE Healthcare (Chicago, IL, USA), Nichirei Biosciences (Tokyo, Japan), and Dako (Glostrup, Denmark).
The other reagents used were obtained from Wako Pure Chemical Industries (Osaka, Japan).

Yurube T., Hirata H., Ito M., Terashima Y., Kakiuchi Y., Kuroda R, & Kakutani K. (2021). Involvement of Autophagy in Rat Tail Static Compression-Induced Intervertebral Disc Degeneration and Notochordal Cell Disappearance. International Journal of Molecular Sciences, 22(11), 5648.

+ Open protocol

+ Expand

Differentiation of Adipocytes with Hormones

Check if the same lab product or an alternative is used in the 5 most similar protocols

High glucose (25 mM) Dulbecco's modified eagle's medium (DMEM; D5796), cell culture grade water, N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (dbcAMP), dexamethasone (Dex) and 3,3′,5-Triiodo-l-thyronine sodium salt (T3) were all purchased from Sigma-Aldrich (Poole, UK). Human Recombinant Des(1–3) IGF-I was purchased from SourceBioscience and other cell culture reagents were purchased from Fisher Scientific (Loughborough, UK). Compounds were prepared as follows: dbcAMP was dissolved in cell culture grade water at 100 mM, Des(1–3) IGF-I in 0.1 M acetic acid at 100 μg/ml, T3 in 0.1 M sodium hydroxide at 100 nM and Dex in absolute ethanol at 10 mM. Compounds were filter-sterilised through a 0.2 μm filter and aliquots stored at −20 °C.

Brearley M.C., Loczenski-Brown D.M., Loughna P.T., Parr T, & Brameld J.M. (2021). Response of the porcine MYH4-promoter and MYH4-expressing myotubes to known anabolic and catabolic agents in vitro. Biochemistry and Biophysics Reports, 25, 100924.

+ Open protocol

+ Expand

Nanoparticle Formulation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ade (≥99%, A9251), ANP rat (amino acid sequence: Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Ile-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr, ≥97%, HPLC, A1663), OA (≥99%, GC, O1008), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 39391), dimethyl sulfoxide (DMSO, D 2650), Dulbecco’s Modified Eagle’s Medium (DMEM, D5796), fetal bovine serum (FBS, F9665), and 3-[4,5-dimethylthiazol-2yl]-2,5diphenyltetrazolium (MTT, M2128) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). DSPE-PEG (MW~5 kDa)-COOH (DSPE-PEG₅₀₀₀-COOH, PS2-ODE-5K) and DSPE-PEG-FITC (PS2-DEFT-5K) were obtained from Shanghai Pengsheng Biological Co., Ltd. (Shanghai, China). Injectable soyabean lecithin (ISL, TW-SL-IJ01) was provided by Shanghai Taiwei Pharmaceutical Co., Ltd. (Shanghai, China).

Yu J., Li W, & Yu D. (2018). Atrial natriuretic peptide modified oleate adenosine prodrug lipid nanocarriers for the treatment of myocardial infarction: in vitro and in vivo evaluation. Drug Design, Development and Therapy, 12, 1697-1706.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!