We purchased reagents from the following suppliers: kaempferol (3,4′,5,7-tetrahydroxyflavone), anti-β-actin antibody, essentially fatty acid-free bovine serum albumin (BSA), transferrin, Hoechst 33258, 7-amino-actinomycin D (7-AAD), and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) (Sigma, St. Louis, MO, USA); selenium and DMEM/Ham’s F-12 nutrient mixture (DMEM/F-12; Gibco BRL, Gaithersburg, MD, USA); phycoerythrin conjugated Annexin V (BD Pharmingen, Franklin Lake, NJ, USA); antibodies against Bcl-2, Bcl-xL, Bax, FAS, FAS-L and α-tubulin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); antibodies against cleaved caspase-9, cleaved caspase-3, cleaved caspase-7, cleaved PARP, Bik, Bak, Bim, Bad, Bid, Akt, and phospho-Akt (Cell Signaling, Beverly, MA, USA); antibodies against cytochrome c and caspase-8 (BD Biosciences, San Jose, CA, USA).
Phycoerythrin conjugated annexin 5
Manufactured by BD
Sourced in United States
Phycoerythrin conjugated Annexin V is a fluorescently labeled protein used for the detection of apoptosis in cells. Annexin V binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. The phycoerythrin dye attached to the Annexin V protein allows for the visualization and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Selenium and dmem ham s f 12 nutrient mixture dmem f 12, by Thermo Fisher Scientific (2 mentions)
Transferrin, by Merck Group (2 mentions)
Antibodies against cleaved caspase 9, by Cell Signaling Technology (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 7, by Cell Signaling Technology (1 mentions)
7 aminoactinomycin d 7 aad, by Merck Group (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Methyl 3h thymidine, by Cytiva (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Protein a sepharose, by Cytiva (1 mentions)
Recombinant human igf2, by R&D Systems (1 mentions)
Horse radish peroxidase conjugated anti rabbit and anti mouse igg, by Cytiva (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Annexin 5, by BD (1 mentions)
3 protocols using phycoerythrin conjugated annexin 5
1
Apoptosis Pathway Protein Analysis
2
Cell Signaling Pathway Analysis
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Kaempferol, essentially fatty acid-free bovine serum albumin, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 7-amino-actinomycin D (7-AAD), phosphatidylinositol, transferrin and anti-β-actin antibody were obtained from Sigma (St. Louis, MO, USA); selenium and DMEM/Ham’s F-12 nutrient mixture (DMEM/F-12 were purchased from Gibco BRL (Gaithersburg, MD, USA)); [methyl-3H]thymidine, protein A-Sepharose and horse-radish peroxidase-conjugated anti-rabbit and anti-mouse IgG were from Amersham (Arlington Heights, IL, USA); antibodies against HRG, IGF-IRβ, p85 and ErbB3 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); antibodies against Akt, phospho (P)-Akt, ERK-1/2 and P-ERK-1/2 were from Cell Signaling Technology (Beverly, MA, USA); anti-phosphotyrosine-RC20 (PY20) antibody linked to horse-radish peroxidase was purchased from BD Transduction Laboratories (Palo Alto, CA, USA); anti-IGF-II antibody was from Amano International Enzyme (Troy, VA, USA); recombinant human HRG-β and recombinant human IGF-II were purchased from R&D Systems, (Minneapolis, MN, USA); phycoerythrin-conjugated Annexin V was from BD Pharmingen, (Franklin Lake, NJ, USA); and [γ-32P]ATP was obtained from PerkinElmer Life and Analytical Sciences, Inc. (Boston, MA, USA).
3
Apoptosis Detection in Virus-Infected Cells
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HeLa S3 cells were detached 80 h after adenovirus infection, with 0.25% trypsin and 1 mM EDTA, washed with PBS, and resuspended in 50 mM Tris-HCl (pH 8.0), 80 mM NaCl, 0.2% BSA, and 2.5 mM CaCl2. Cells were subsequently incubated with phycoerythrin-conjugated Annexin V (BD Biosciences, San Jose, CA, USA). Annexin V-positive cells were detected by flow cytometry at 564-606 nm, using a FACSCalibur flow cytometer (BD Biosciences).
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