PMN and lymphocyte cell populations within lacrimal glands, draining lymph nodes, and corneas were assessed by flow cytometry as previously described 14 . Anti-FcR mAB (BD PharMingen, San Diego, CA) was used to block Fc receptors for 10 minutes before samples were incubated for 30 minutes in titrated amounts of fluorescent-labeled antibodies: FITC-conjugated anti-Ly6g (1A8 clone; BD PharMingen, San Diego, CA, USA) for PMN; PE-conjugated anti-CD45 (MEC 13.3 clone; BD PharMingen) for leukocytes; PE-conjugated anti-CD3 (500A2 clone; BD PharMingen) for T cells; FITC-conjugated anti-CD4 (RM4–5 clone; BD PharMingen) for activated CD4+ T cells; APC-conjugated anti-IFN-γ (XMG1.2; TONBO biosciences); FITC-or APC conjugated anti-IL17 (eBio17B7; ebioscience); APC conjugated anti-Foxp3 (3G3; TONBO biosciences). Live cells were sorted using a high-speed sorter (MoFlo, SX DakoCytomation, Inc., Fort Collins, CO, USA).
Fitc or apc conjugated anti il17 ebio17b7
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC-or APC conjugated anti-IL17 (eBio17B7) is a lab equipment product. It is a fluorescently labeled antibody that binds to the interleukin-17 (IL-17) protein. This product can be used for the detection and analysis of IL-17 in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti fcr mab, by BD (2 mentions)
Fitc conjugated anti ly6g clone 1a8, by BD (2 mentions)
Pe conjugated anti cd45 mec 13.3 clone, by BD (2 mentions)
Pe conjugated anti cd3 500a2 clone, by BD (2 mentions)
Fitc conjugated anti cd4 rm4 5 clone, by BD (2 mentions)
Apc conjugated anti ifn γ xmg1.2, by Cytek Biosciences (2 mentions)
Apc conjugated anti foxp3 3g3, by Cytek Biosciences (2 mentions)
2 protocols using fitc or apc conjugated anti il17 ebio17b7
1
Assessing Immune Cell Populations in Ocular Tissues
2
Assessing Immune Cell Populations in Ocular Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PMN and lymphocyte cell populations within lacrimal glands, draining lymph nodes, and corneas were assessed by flow cytometry as previously described 14 . Anti-FcR mAB (BD PharMingen, San Diego, CA) was used to block Fc receptors for 10 minutes before samples were incubated for 30 minutes in titrated amounts of fluorescent-labeled antibodies: FITC-conjugated anti-Ly6g (1A8 clone; BD PharMingen, San Diego, CA, USA) for PMN; PE-conjugated anti-CD45 (MEC 13.3 clone; BD PharMingen) for leukocytes; PE-conjugated anti-CD3 (500A2 clone; BD PharMingen) for T cells; FITC-conjugated anti-CD4 (RM4–5 clone; BD PharMingen) for activated CD4+ T cells; APC-conjugated anti-IFN-γ (XMG1.2; TONBO biosciences); FITC-or APC conjugated anti-IL17 (eBio17B7; ebioscience); APC conjugated anti-Foxp3 (3G3; TONBO biosciences). Live cells were sorted using a high-speed sorter (MoFlo, SX DakoCytomation, Inc., Fort Collins, CO, USA).
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