Urine NAG was measured using the NAG assay kit (Bio-quant, CA, USA). Urine creatinine was determined using a creatinine colorimetric detection kit (Enzo Life Sciences, NY, USA). Fibrinogen was measured using a Luminex-based assay kit (Millipore, MA, USA). Urinary protein biomarkers of Kim-1, NGAL, albumin, osteopontin, α-GST, GST-Yb1, RPA-1 and clusterin were measured using the rat multiplex assay kits and SECTOR™ Imager 2400 electrochemiluminescence detection platform (Meso Scale Discovery, MD, USA).
Luminex based assay kit
Manufactured by Merck Group
Sourced in United States
The Luminex-based assay kit is a multiplex detection system that enables the simultaneous measurement of multiple analytes in a single sample. The core function of this product is to provide a platform for the quantitative analysis of proteins, cytokines, and other biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using luminex based assay kit
1
Comprehensive Urinary Biomarker Assessment
2
Kidney Function Evaluation Protocol
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To evaluate kidney function, we measured serum blood urea nitrogen (BUN) and creatinine (Cre) with commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's protocols. The commercial kit for measurement of Cre was based on creatinine acid oxidase method as previous reports [40 (link),41 (link)]. The levels of urine Fg were measured using commercially available species-specific Luminex-based assay kit from Millipore (Billerica, MA, USA). The levels of serum Cystatin C were analyzed by a mouse Cystatin C commercial kit (Elabscience Biotechnology Co.,Ltd, Wuhan, China). Subsequently, the optical density for measurement of serum Cystatin C was measured using a microplate reader (SYNERGY H4, BioTek, VT, USA) at the absorbance of 450 nm.
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