Bs 6982r

Manufactured by Bioss Antibodies

Sourced in United States

The Bs-6982R is a compact and versatile laboratory centrifuge designed for a wide range of applications. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 4,000 x g. The Bs-6982R can accommodate a variety of sample volumes and tube sizes, making it a reliable tool for various laboratory procedures.

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Lab products found in correlation

5 protocols using bs 6982r

Histological Evaluation of Intestinal Colitis

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To evaluate the severity of colitis, the whole large intestine was harvested, fixed in 10% formalin for 15 h, then, embedded in paraffin. Paraffin sections (4 μm) were stained with hematoxylin and eosin. The sections were also reacted with various antibodies, AdipoR1 (5512–1; Epitomics, Burlingame, CA, United States), CD68 (ab125212; abcam, Cambridge, United Kingdom), cyclooxygenase/cox2 (SP21; Spring Bioscience, Inc., Pleasanton, CA, United states) and neutrophil elastase (bs-6982R; Bioss, Inc.). The endogenous peroxidase activity and non-specific binding were blocked by 0.3% H₂O₂/ methanol and 10% calf serum/PBS. The signal was detected by a horseradish peroxidase (HRP)/ diaminobenzidine (DAB) system according to the manufacturer’s instructions (Dako, Carpinteria, CA, United States).

Peng Y.J., Shen T.L., Chen Y.S., Mersmann H.J., Liu B.H, & Ding S.T. (2018). Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. Journal of Biomedical Science, 25, 24.

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Immunofluorescence Analysis of Tumor Infiltrates

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FFPE tumor sections were processed as described above. Antigen retrieval was performed using citrate buffer (PH = 6.0). Primary antibodies used were as follows: rabbit anti-mouse NE (1:100, BS-6982 R, Bioss), rabbit anti-mouse MPO (1:1000, ab188211, Abcam). The primary antibodies were detected with FITC (ZF-0311, ZhongShanJinQiao Co., LTD) or Cy5 (ab6564, Abcam) conjugated goat anti-rabbit IgG, respectively. Then, DAPI was used for counterstaining the nuclei, and images were obtained by laser scanning confocal microscopy. The number of NE positive cells was counted by Image-Pro Plus (version 4.5, USA).

Tan Q., Ma X., Yang B., Liu Y., Xie Y., Wang X., Yuan W, & Ma J. (2022). Periodontitis pathogen Porphyromonas gingivalis promotes pancreatic tumorigenesis via neutrophil elastase from tumor-associated neutrophils. Gut Microbes, 14(1), 2073785.

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Western Blot Analysis of Signaling Proteins

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Tissues or cells were lysed with RIPA buffer, separated by SDS-PAGE gel electrophoresis and then transferred to a PVDF membrane. The proteins were then immunoblotted with the primary antibody: anti-SGK1 (bms-33275 M, Bioss), anti-pSTAT3 (bs-16558R, Bioss), anti-STAT3 (ab109085, Abcam), anti-NRF2 (bs-1074R, Bioss), anti-ERK1 + ERK2 (ab184699, Abcam), anti-pERK1 + pERK2 (ab214036, Abcam), anti-β-actin (4970, Cell Signaling Technology), anti-MPO (ab208670, Abcam), anti-CitH3 (ab281584, Abcam), anti-NE (bs-6982R, Bioss), anti-PAD4 (ab214810, Bioss). This was followed by color development using horseradish peroxidase (HRP)-enhanced chemiluminescence (ECL) assays.

Li X., Wang Z., Jiao C., Zhang Y., Xia N., Yu W., Chen X., Wikana L.P., Liu Y., Sun L., Chen M., Xiao Y., Shi Y., Han S, & Pu L. (2023). Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. Journal of Translational Medicine, 21, 121.

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Immunoprecipitation of G-CSF and EP300

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KG1a cells were lysed by the non-denaturing lysis buffer. Next, the supernatant of cell lysis was pre-cleaned with protein A/G magnetic beads (Thermo Fisher Scientific) for 2 h at 4 °C. Subsequently, about 300 μg of protein were incubated with 1μg G-CSF antibody (#ab181053, Abcam) or EP300 antibody (#ab275378, Abcam) and 25 microliters of protein A/G magnetic beads for immunoprecipitation at 4 °C overnight. Following the incubation with antibody and protein A/G magnetic beads, protein A/G magnetic beads were collected using magnetic separation device (Thermo Fisher Scientific), and precipitated complexes were cleansed by washing buffer (Thermo Fisher Scientific). Finally, bound proteins were analyzed by WB using anti-ELANE (1:500, # bs-6982R, Bioss) or anti-TPO (1:500, # ab196026, Abcam). Rabbit IgG was used for negative control.

Wang X., Liu X, & Wang H. (2022). Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells. Cancer Gene Therapy, 29(11), 1742-1750.

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Protein Expression Analysis by Western Blot

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After treatment, the cells were harvested, and the proteins were extracted by whole cell lysis (Beyotime). Protein concentration was determined by BCA reagent from Pierce. Lysates were eluted in 1 × SDS Loading Buffer (Beyotime), resolved by 10% SDS-polyacrylamide gels, transferred to PVDF membranes (Bio-Rad) and then probed with the appropriate antibodies. The LumiGlo Chemiluminescent Substrate System (Thermo) was used for protein detection. The antibodies used were specific for Cyclin D1 (bs-0572R, Bioss), p21 (sc-6246; Santa Cruz), Alix (18269, CST), Flotillin-1 (18634, CST), Annexin V (8555, CST), Neutrophil Elastase (63610,CST), Neutrophil Elastase (bs-6982R, Bioss), p38 MAPK (9212, CST), p-p38 MAPK (T180/Y182) (9215, CST), p-STAT3 (Y705) (9145, CST), STAT3 (9132; CST), VDR (14526-1-AP; Proteintech), GAPDH (1:10,000; MB001H, Bioworld) and β-actin (1:10,000; ab133626, Abcam). All antibodies were used at a 1:1000 dilution for Western blotting unless otherwise noted.

Sun L., Yang N., Chen B., Bei Y., Kang Z., Zhang C., Zhang N., Xu P., Yang W., Wei J., Ke J., Sun W., Li X, & Shen P. (2023). A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy. Acta Pharmaceutica Sinica. B, 13(7), 3027-3042.

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