Pe anti human cd33 antibody

Manufactured by BioLegend

The PE anti-human CD33 antibody is a fluorescently labeled monoclonal antibody that specifically binds to the CD33 antigen expressed on the surface of human cells. This reagent can be used for the identification and enumeration of CD33-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Apc anti human cd19 antibody, by BioLegend (2 mentions) Facsaria 2 machine, by BD (1 mentions) Human trustain fcx, by BioLegend (1 mentions) Pe cy7 antihuman cd3 antibody, by BioLegend (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions)

Spelling variants of this product

CD33-PE (11 citations) Anti-CD33/PE (4 citations) Anti-CD33 (4 citations) Anti-human CD33 (3 citations) PE anti-human CD33 (2 citations)

4 protocols using pe anti human cd33 antibody

Multiparameter Flow Cytometry Analysis of Bone Marrow

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of cells harvested from bone marrow, cells were first
incubated with blocking antibodies (Human TruStain FcX, BioLegend; 422302
and Mouse TruStain fcX anti-mouse CD16/32, BioLegend; 101320) for 15 min,
followed by incubation with V450 Mouse Anti-Human CD45 Clone HI30 (BD
Biosciences, 560367), PE-eFluor 610 mCD45 Monoclonal Antibody (30-F11)
(Thermo Fisher, 61-0451-82), FITC anti-human CD235a Antibody (BioLegend,
349104), PE anti-human CD33 Antibody (366608, BioLegend), APC anti-human
CD19 Antibody (BioLegend, 302212), PE/Cy7 anti-human CD3 antibody
(BioLegend, 300420), FITC anti-human CD34 antibody (BioLegend, 343504) and
Fixable Viability Dye eFluor 780 for live/dead staining (Thermo Fisher,
65-0865-14). Percentage human engraftment was calculated as hCD45+
cells/(hCD45+ cells + mCD45+ cells). B cells (CD19+) and myeloid (CD33+)
lineages were gated on the hCD45+ population. HSPCs (CD34+) and T cells
(CD3+) were gated on the hCD45+CD19-CD33-population.
hCD45+CD19-CD33-CD34-CD3-population was referred as lineage-. FACS analysis
and sorting of CD34+ / CD19+ cells were done using the FACSAria II machine
(BD Biosciences).

Nagpal N., Wang J., Zeng J., Lo E., Moon D.H., Luk K., Braun R.O., Burroughs L.M., Keel S.B., Reilly C., Lindsley R.C., Wolfe S.A., Tai A.K., Cahan P., Bauer D.E., Fong Y.W, & Agarwal S. (2020). Small molecule PAPD5 inhibitors restore telomerase activity in patient stem cells. Cell stem cell, 26(6), 896-909.e8.

+ Open protocol

+ Expand

Peptide E5 Identification and Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peptide E5 (GGRSFFLLRRIQGCRFRNTVDD) used in this study was identified by the cell-based selection24 (link) and purchased from GL Biochem (Shanghai) Ltd. The stock solution of E5 was dissolved in the sterile water. PE anti-human CD33 antibody was purchased from BioLegend (San Diego, CA).

Li X., Guo H., Duan H., Yang Y., Meng J., Liu J., Wang C, & Xu H. (2015). Improving chemotherapeutic efficiency in acute myeloid leukemia treatments by chemically synthesized peptide interfering with CXCR4/CXCL12 axis. Scientific Reports, 5, 16228.

+ Open protocol

+ Expand

Engrafting Human Hematopoietic Cells in NSG Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male and female 8- to 12-week-old NOD-SCID Il2rg-/- (NSG) mice were used as recipient mice. Immediately prior to transplantation, NSG mice received a single dose of 2Gy irradiation. 1-3 million hPSC-derived hematopoietic progenitors, or alternatively, 80,000-100,000 human CD34+ cord blood hematopoietic stem and progenitor cells were transplanted intrafemorally in 20-30 μL IMDM + 20% FBS into irradiated NSG mice. 3-6 months post-transplantation, mice were sacrificed, and bone marrow was analyzed by flow cytometry to detect the presence of human cells. The following antibodies were used to detect human hematopoietic cells in transplanted mice via flow cytometry: BD Horizon V450 anti-human CD45 antibody (BD Biosciences, 560367), APC-Cy7 anti-Human HLA-A/B/C antibody (Biolegend, 311425), PE anti-Human CD33 antibody (Biolegend, 303404), and APC anti-Human CD19 antibody (Biolegend, 302212).

Fowler J.L., Zheng S.L., Nguyen A., Chen A., Xiong X., Chai T., Chen J.Y., Karigane D., Banuelos A.M., Niizuma K., Kayamori K., Nishimura T., Cromer M.K., Gonzalez-Perez D., Mason C., Liu D.D., Yilmaz L., Miquerol L., Porteus M.H., Luca V.C., Majeti R., Nakauchi H., Red-Horse K., Weissman I.L., Ang L.T, & Loh K.M. (2024). Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells. Developmental Cell, 59(9), 1110-1131.e22.

+ Open protocol

+ Expand

Identification of HL-60 Cells by CD33

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peripheral blood and bone marrow from femur flushing were collected and erythrocytes were excluded using blood cell lysis buffer (Beckman Coulter, Krefeld, Germany). PE anti-human CD33 antibody (BioLegend, San Diego, CA) was used to identify HL-60 cells via flow cytometry (C6 Accuri flow cytometer, BD Company, Franklin Lakes, NJ) according to the manufacturer’s protocol.

Ma Q., Wang C., Li X., Guo H., Meng J., Liu J, & Xu H. (2016). Fabrication of water-soluble polymer-encapsulated As4S4 to increase oral bioavailability and chemotherapeutic efficacy in AML mice. Scientific Reports, 6, 29348.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!