Anti cd4 sk3

Manufactured by BD

Anti-CD4 (SK3) is a monoclonal antibody that recognizes the CD4 antigen, which is expressed on the surface of T helper cells. It is a widely used tool in flow cytometry and other immunological applications for the identification and analysis of CD4+ T cells.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd8 sk1, by BD (3 mentions) Cytofix cytoperm kit, by BD (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (1 mentions) Sh800, by Sony (1 mentions) Anti tnf α mp6 xt22, by Thermo Fisher Scientific (1 mentions) Anti ifnγ xmg1.2, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Monensin, by BioLegend (1 mentions) Anti tnf α mab11, by BD (1 mentions) Brefeldin a, by Merck Group (1 mentions) Cytoflex, by Beckman Coulter (1 mentions) Live dead fixable near ir dead cell stain, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Facsdiva, by BD (1 mentions)

Spelling variants of this product

Anti-CD4 (255 citations) CD4 (SK3; (14 citations) Anti-CD4-PerCP (clone SK3, (7 citations) Anti-CD4 PE Cy7 (clone SK3- (2 citations) PE-Cy7-conjugated anti-CD4 (clone SK3, (2 citations) PerCP-Cy5.5-conjugated anti-CD4 (SK3; (2 citations) Anti-CD4 clone SK3 (2 citations) Anti-human CD4 (SK3; (2 citations) Anti-human CD4-phycoerythrin (SK3; (2 citations)

4 protocols using anti cd4 sk3

PBMC Cell Sorting and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell sorting was performed from PBMC fractions using FACSAria II (Becton Dickinson) or Sony SH800 (Sony Biotechnology Inc.). For sorting, PBMC cells were stained with anti-CD3 (SK7, BD, cat. no 345767 or 557851), anti-CD4 (SK3, BD, cat. no 345770), anti-CD8 (SK-1, BD, cat. no 335822 or 345772) and the appropriate anti-Vβ antibody. Purities of the sorted cell fractions were controlled with flow cytometry, and the purities were nearly 100%.

Savola P., Kelkka T., Rajala H.L., Kuuliala A., Kuuliala K., Eldfors S., Ellonen P., Lagström S., Lepistö M., Hannunen T., Andersson E.I., Khajuria R.K., Jaatinen T., Koivuniemi R., Repo H., Saarela J., Porkka K., Leirisalo-Repo M, & Mustjoki S. (2017). Somatic mutations in clonally expanded cytotoxic T lymphocytes in patients with newly diagnosed rheumatoid arthritis. Nature Communications, 8, 15869.

+ Open protocol

+ Expand

Multiparametric T Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse T cells were labeled for 20min at 4°C with anti-CD3 (17A2), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD45.1 (A20), anti-CD45.2 (104), anti-Vβ_5.1–5.2 (MR9–4), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CD127 (A7R34), KLRG1 (MAFA), anti-IFN-γ (XMG1.2) and anti-TNF-α (MP6-XT22) (all eBioscience). Human T cells were labeled with anti-CD3 (UCHT1, Beckman Coulter), anti-CD4 (SK3, BD Biosciences), anti-CD8 (SK1, BD Biosciences). Dead cells were excluded with 1ng/ml TO-PRO-3 iodide (Sigma-Aldrich), or Near-IR (L10119, Life Technology). Intracellular cytokine staining was performed with the cytofix/cytoperm kit (BD Biosciences). Flow cytometry analysis was performed on LSR-II and LSR Fortessa (BD Biosciences). Data were analyzed with FlowJo software (Tree Star, version 7.6.5 and version 10). Representative flow cytometry gating strategies are found in Fig S9.

Salerno F., Engels S., van den Biggelaar M., van Alphen F.P., Guislain A., Zhao W., Hodge D.L., Bell S.E., Medema J.P., von Lindern M., Turner M., Young H.A, & Wolkers M.C. (2018). Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nature immunology, 19(8), 828-837.

+ Open protocol

+ Expand

In Vitro SARS-CoV-2 T Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs were stimulated with peptide pools and expanded in vitro for 10 days as described before (23 (link)). Expanded T cell lines were stimulated for 5 hours at 37°C with or without SARS-CoV-2 peptide pools (2 μg/mL). After 1 hour, 10 μg/mL brefeldin A (MilliporeSigma) and 1× monensin (BioLegend) were added. Cells were stained with the yellow LIVE/DEAD fixable dead cell staining kit (Invitrogen, Thermo Fisher Scientific) and the surface markers anti-CD3 (SK7 or OKT3; BioLegend), anti-CD4 (SK3, BD Biosciences), and anti-CD8 (SK1, BD Biosciences). Cells were subsequently fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences) and stained with anti–IFN-γ (25723; R&D Systems) and anti–TNF-α (MAb11, BD Biosciences) antibodies and analyzed on a CytoFLEX (Beckman Coulter). Data were analyzed by FlowJo (BD Biosciences).

Samandari T., Ongalo J.B., McCarthy K.D., Biegon R.K., Madiega P.A., Mithika A., Orinda J., Mboya G.M., Mwaura P., Anzala O., Onyango C., Oluoch F.O., Osoro E., Dutertre C.A., Tan N., Hang S.K., Hariharaputran S., Lye D.C., Herman-Roloff A., Le Bert N, & Bertoletti A. (2023). Prevalence and functional profile of SARS-CoV-2 T cells in asymptomatic Kenyan adults. The Journal of Clinical Investigation, 133(13), e170011.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling from BAL Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

BAL samples were passed through a cell strainer to remove debris and any clumps of tissue. After washing, cell pellets were resuspended in 1 mL of RPMI-1640 (Thermo Fisher Scientific) and manually counted using a hemocytometer. Each sample was resuspended in FACS buffer (PBS plus 2% fetal bovine serum (Thermo Fisher Scientific) plus 10% human serum for 20 minutes on ice, followed by staining with LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (Thermo Fisher) for 15 minutes in the dark to allow the exclusion of dead cells from the analysis. After washing, a cocktail of the antibodies listed below was added and staining completed in the dark for 25 minutes before fixing cells in 2% paraformaldehyde in PBS overnight. Samples were resuspended in FACS buffer, then analyzed on a BD LSRFortessa™ X-20 using BD FACS-Diva (BD Biosciences). Antibody cocktail (all antibodies from BD Biosciences): anti-CD3 UCHT1 BUV737, anti-CD4 SK3 (aka: Leu3a) BV480, anti-CD8 RPA-T8 PE, anti-CD19 HIB19 PerCP-Cy5.5, anti-CD45 HI30 BUV395, anti-CD56 NCAM16.2 BV711, anti-TCRγ/δ-1 11F2 FITC, MR1-5-OP-RU Tetramer (hereafter MR1-tetramer) conjugated to BV421-streptavidin (BD Horizon) was produced as described [6] .

McElroy R., Talesh G.A., Harpur C.M., Carzino R., Corbett A.J., Pellicci D.G., Ranganathan S, & Sutton P. (2022). Unconventional T Cell Immunity in the Lungs of Young Children with Cystic Fibrosis. Frontiers in bioscience (Landmark edition), 27(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!