Honeysuckle and granulated sugar were purchased from local supermarkets; Lactobacillus acidophilus zrx02 (GenBank No.MF804413) and Lactiplantibacillus plantarum zrx03 (GenBank No.MN784485) were kept by the Food Biotechnology Laboratory of the Food College of Henan Institute of Science and Technology. Gallic acid was purchased from Chengdu Munster Co., Ltd. Folin-phenol and Tris were purchased from Beijing Solarbio Co., Ltd. MRS medium was purchased from Beijing Aoboxing Biotechnology Co., Ltd. The reagents mentioned above are analytically pure.
Folin phenol
Manufactured by Solarbio
Sourced in China
Folin-phenol is a reagent used in colorimetric assays to quantify the total phenolic content in various samples. It works by reacting with phenolic compounds to produce a blue-colored complex that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Fungal dna kit, by Omega Engineering (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Coomassie brilliant blue kit, by Nanjing Jiancheng (1 mentions)
Phenol, by Solarbio (1 mentions)
Ethyl alcohol, by Solarbio (1 mentions)
Rutin, by Solarbio (1 mentions)
Sep pak c18, by Waters Corporation (1 mentions)
Epicatechin gallate, by Yuanye Bio-Technology (1 mentions)
Catechin, by Yuanye Bio-Technology (1 mentions)
Gallocatechin, by Yuanye Bio-Technology (1 mentions)
Epicatechin, by Yuanye Bio-Technology (1 mentions)
Epigallocatechin, by Yuanye Bio-Technology (1 mentions)
Methanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Epigallocatechin gallate (egcg), by Yuanye Bio-Technology (1 mentions)
5 protocols using folin phenol
1
Microbial Fermentation of Honeysuckle for Bioactive Compounds
2
Broad Bean Protein Hydrolysis Protocol
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Commercial neutral protease, with high purity levels and a strong ability to hydrolyze protein, was acquired from Beijing Solarbio Science & Technology Company (Beijing, China), which conforms to the Food Chemical Codex (FCC) and the food grade enzymes specification standards of light industry (QB/T1803-93). The fungal DNA kit was purchased from OMEGA Engineering (Connecticut, America); DEAE-Sepharose Fast Flow was obtained from Beijing Ruida Henhui Science & Technology Development Company (Beijing, China); and the Coomassie brilliant blue Kit was from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Polyethylene glycol 2000-0 (PEG-20M) was obtained from Chengdu Jinshan Chemical Reagent Company (Chengdu, China). Tris, Folin phenol, and Triton X-100 were purchased from Beijing Solarbio Science & Technology Company (Beijing, China). Rose Bengal medium was obtained from Beijing Aobox Biotechnology Company (Beijing, China). All the other chemicals were analytical grade.
The fermented broad beans were collected under high temperature in summer from an open-air natural fermentation workshop, where the average temperature of the fermentation pool reaches 55 °C.
The fermented broad beans were collected under high temperature in summer from an open-air natural fermentation workshop, where the average temperature of the fermentation pool reaches 55 °C.
3
Foxtail Millet Cultivation Protocol
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Jingu 21, a representative elite foxtail millet cultivar with high yield and high quality, was used in the experiment. The seeds were obtained from the Millet Research Institute of Shanxi Agriculture University (Changzhi, China).
Ethyl alcohol, Phenol, Thymol blue, Folin Phenol, and Rutin were purchased from Beijing Solarbio Science & TechnologyCo., Ltd., Beijing, China. NaNO2, Al(NO3)3, KOH, NaOH, and sulfuric acid were purchased from Guoyao Group Chemical Reagent Co., Ltd., Beijing, China.
Ethyl alcohol, Phenol, Thymol blue, Folin Phenol, and Rutin were purchased from Beijing Solarbio Science & TechnologyCo., Ltd., Beijing, China. NaNO2, Al(NO3)3, KOH, NaOH, and sulfuric acid were purchased from Guoyao Group Chemical Reagent Co., Ltd., Beijing, China.
4
Comprehensive Chromatographic Analysis of Antioxidants
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Chromatography-grade acetonitrile was purchased from Dima Technologies (Beijing, China). Analytical-grade methanol and anhydrous ethanol (≥99.8%) were obtained from Beijing Chemistry Co. (Beijing, China). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from TSI AI Chemical Industry Development (Shanghai, China). Folin-phenol was obtained from Solarbio (Beijing, China). Nutritive agar was obtained from Aoboxing Biotechnology (Beijing, China). Sodium fluorescein, 2-aminopropane, sodium hypochlorite, potassium hypophosphite, dipotassium phosphate, and vitamin E (Ve) were obtained from Mclean Biochemical Technology (Shanghai, China). Bisdemethoxycurcumin standard was obtained from Solarbio (Beijing, China). Macroporous resin (XAD-7) was obtained from Abol Biotechnology Co., Ltd (Beijing, China). Solid-phase extraction (SPE) cartridges (Sep-Pak C18; 12 mL, 2000 mg) were obtained from Waters (Milford, USA). Syringe filter units (0.22 μm) were supplied by Hercules (Beijing, China). Distilled water was used throughout.
5
Polyphenol Quantification and Characterization
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The (+)-catechin, (−)-epicatechin, (−)-epigallocatechin, and (−)-epicatechin gallate (+)-gallocatechin and (−)-epigallocatechin gallate, (purity > 98 %) were purchased from Shanghai Yuanye Biotechnology Co. ltd (Shanghai, China). Methanol, acetonitrile, acetic acid, formic acid, hydrochloric acid (HPLC grade), gallic acid, rutin, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Shanghai, China). Phloroglucinol and ascorbic acid were purchased from Shandong Xiya Chemistry Industry ltd (Linyi, China). Sodium dodecyl sulfate (SDS), triethanolamine (TEA), and Folin-phenol were purchased from Beijing Solarbio Science & Technology Co., ltd (Beijing, China). Sodium carbonate, sodium hydroxide, aluminum chloride, sodium nitrite, and sodium acetate were purchased from Tianjin Bodi Chemical Co., ltd (Tianjin, China). The C-18 SPE column was obtained from Tianjin Chengzhou Technology Co. ltd (Tianjin, China). The deionized water was obtained from a Milli-Q system (Merck Millipore, Darmstadt, Germany).
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