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Projet 1200

Manufactured by 3D Systems
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Sourced in United States

The ProJet 1200 is a desktop 3D printer designed for precision micro-parts and jewelry casting patterns. It uses stereolithography (SLA) technology to create accurate and detailed models from liquid photopolymer resin. The ProJet 1200 has a build volume of 43 x 27 x 150 mm and can produce parts with a layer thickness of 30 microns.

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Lab products found in correlation

Inventor pro edition, by Autodesk (2 mentions) Fusion 360, by Autodesk (1 mentions)

8 protocols using projet 1200

1

Chronic Neural Electrode Implantation in Rats

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Rats underwent surgery to implant chronic recording electrodes. Four animals were implanted for HC recordings with 32-channel silicon probes arranged as tetrodes with 25 μm between adjacent electrode sites and impedances of 1.23 ± 0.32 MΩ. The eight tetrodes were distributed across four shanks at tip-to-tetrode depths of 78 μm and 228 μm (NeuroNexus A4X2-tet-5mm). Shanks were separated by 200 μm giving each probe a total length of 0.67 cm. During implantation, the long axis was oriented medial-lateral to sample proximal CA1 and CA2 of dorsal HC. Two animals were implanted for dual-site recordings with stainless steel wire electrodes (0.42 ± 0.25 MΩ) using custom designed (Autodesk Inventor) and 3-D printed implant bodies (3D Systems ProJet 1200) to house the electrodes coupled to an integrated electrode interface board. These implants comprised a grid of 18 electrodes targeting HC (2 x 2.5 mm footprint, 4 electrode rows, 0.5 mm center-to-center) and 14 electrodes arranged in two parallel rows (4 x 1 mm footprint, 0.5 mm long-axis spacing) aligned to the rostrocaudal axis of mPFC.
Schultheiss N.W., Schlecht M., Jayachandran M., Brooks D.R., McGlothan J.L., Guilarte T.R, & Allen T.A. (2020). 'Awake delta' and theta-rhythmic hippocampal network modes during intermittent locomotor behaviors in the rat. Behavioral neuroscience, 134(6), 529-546.
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2

3D-Printed Cannula System for Chronic Neural Implants

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A cannula implant system was created using a high-resolution (56 μm) stereolithography 3D printer (ProJet 1200; 3D Systems), suitable for chronic head stages. A custom-designed 3D-printed cannula assembly was created using CAD software (Autodesk Inventor Pro Edition) and assembled with guide cannulas (27 gauge; outer diameter 0.41 mm; inner diameter 0.31 mm; Component Supply Company, FL) targeting the PER bilaterally (A/P −6.0 mm, M/L ± 6.8 mm, D/V −6.0 mm) and a single site aimed at RE (at a 10° angle to avoid the superior sagittal sinus; A/P −1.8 mm, M/L −1.2 mm, D/V −6.7 mm).
Jayachandran M., Linley S.B., Schlecht M., Mahler S.V., Vertes R.P, & Allen T.A. (2019). Prefrontal Pathways Provide Top-Down Control of Memory for Sequences of Events. Cell reports, 28(3), 640-654.e6.
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3

3D-Printed Cannula System for Chronic Neural Implants

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A cannula implant system was created using a high-resolution (56 μm) stereolithography 3D printer (ProJet 1200; 3D Systems), suitable for chronic head stages. A custom-designed 3D-printed cannula assembly was created using CAD software (Autodesk Inventor Pro Edition) and assembled with guide cannulas (27 gauge; outer diameter 0.41 mm; inner diameter 0.31 mm; Component Supply Company, FL) targeting the PER bilaterally (A/P −6.0 mm, M/L ± 6.8 mm, D/V −6.0 mm) and a single site aimed at RE (at a 10° angle to avoid the superior sagittal sinus; A/P −1.8 mm, M/L −1.2 mm, D/V −6.7 mm).
Jayachandran M., Linley S.B., Schlecht M., Mahler S.V., Vertes R.P, & Allen T.A. (2019). Prefrontal Pathways Provide Top-Down Control of Memory for Sequences of Events. Cell reports, 28(3), 640-654.e6.
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4

Custom Nitinol Stent with Integrated Sensors

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A custom stent was designed using SolidWorks (Dassault, France) and Fusion 360 (AutoDesk, USA). Prototypes were rapid prototyped on a ProJet 1200 SLA printer (3D Systems, USA). A custom Nitinol self‐expanding stent was laser cut with an electro chemical polishing by Meko (MeKo GMBH, Germany). Knowing that silicon sensors with platinum IDEs were capable of cell detection these were packaged into a compact sensor and encapsulated for added protection.[46] A custom stent was created through computer aided design and rapid prototyped through in house 3D printing until a final design was realized. This was passed onto a commercial laser cutting company (Meko, Germany) who created a Nitinol stent with an electropolished surface finish measuring 10 mm internal diameter by 20 mm with a strut thickness of 160 µm the island that holds the sensor 5 × 2 mm 2.1×1 mm hole Figure 6A. The sensor was exposed onto the luminal surface of the stent using a biocompatible epoxy (UV15×6MED‐2, Masterbond, USA) and seeded with MASMCs and MECs.
Hoare D., Tsiamis A., Marland J.R., Czyzewski J., Kirimi M.T., Holsgrove M., Russell E., Neale S.L., Mirzai N., Mitra S, & Mercer J.R. (2022). Predicting Cardiovascular Stent Complications Using Self‐Reporting Biosensors for Noninvasive Detection of Disease. Advanced Science, 9(15), 2105285.
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5

3D Printed Biocompatible Rat Implant

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RatHat components were printed using the 3DSystems ProJet1200, a high-resolution (56-μm xy, 30-μm layer thickness) 3D printer that uses microstereolithography (laser polymerization of resin and UV light-curing), but any high-resolution 3D printer is suitable. With ProJet1200 prints, we use VisiJet FTXGreen resin, a UV-curable and biocompatible plastic composition used in castings because it is durable, with a tensile strength of 30 MPa (or 4351 PSI). After devices are printed, we ensure holes are clear of debris or resin by thoroughly cleaning prints with multiple dips in 70% isopropyl alcohol and clearing holes with pressurized air. Non-printable components such as wires or tubing are secured to the implant device prior to surgery with cyanoacrylate (Zap CA+, Super Glue Corporation) followed by a quick-cure spray (Zip Kicker, Super Glue Corporation). Another advantage of the RatHat is that components are easily assembled using build-specific 3D-printable assembly bases/jigs. All implants are sterilized with 70% ethanol before surgical implantation and a gas sterilizer (ethylene oxide). Autoclaving is not recommended.
Allen L.M., Jayachandran M., Viena T.D., Su M., McNaughton B.L, & Allen T.A. (2020). RatHat: A Self-Targeting Printable Brain Implant System. eNeuro, 7(2), ENEURO.0538-19.2020.
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6

Microfluidic PDMS Cell Culture Chip Fabrication

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Three pieces of the molds were used for microfluidic PDMS chip fabrication, including a base, the side-wall molding, and top magnet holder. Molds were designed by using the SolidWorks® 2017 and printed out by the 3D printer (ProJet 1200 from 3D Systems). The finest structures printed by the 3D printer is in 30 μm. The height of the microfluidic channel is molded at 50 μm. The cylindrical cell culture chamber is molded in 1000-μm diameter and 500-μm height. All 3D-printed molds were sputtering coated with palladium in a thickness of 20 nm for easy release of molded-PDMS. Three pieces of molds were assembled to cast the PDMS microfluidic cell culture device as shown in Figure S1, which was cast by a 10:1 ratio of Dow SYLGARD™ 184 silicone solution (Sigma-Aldrich) and incubated at the temperature of 40 °C for 6 hours. After a complete cure, the molded PDMS polymer can be peeled off easily. The molded cell culture chamber is open to the air and allows a PDMS-made plug to cap on the top. Chip inlets and outlets were formed by punching holes in 0.75 mm diameter. Piranha solution-cleaned glass slides and the PDMS layer were both treated with high-voltage plasma for at least 30 seconds, and then bond on the hot plate at the temperature of 40 °C for 5 mins. The formed microfluidic cell culture chip was cleaned by DI water, and then sterilized using autoclave at 121 °C for 30 mins.
Zhao Z., McGill J., Gamero-Kubota P, & He M. (2019). Microfluidic On-demand Engineering of Exosomes towards Cancer Immunotherapy. Lab on a chip, 19(10), 1877-1886.
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7

Standardized Wax Pattern Fabrication

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The contour and thickness of the wax patterns of Group A were standardized using computer-aided software (Exocad DentalCAD, Darmstadt, Germany). For this, first, the stone die was scanned using a dental scanner (Open technology). The designing of the wax pattern of thickness 0.5 mm was then done using the software (ExoCad). This design was used to fabricate the patterns of Group A using a 3D printer (ProJet 1200, 3D SYSTEMS).
For Group B, a master wax pattern was designed using the same Computer aided design (CAD) data as done for Group A on a separate stone die which was poured from the impression of the master model. Then, a putty index of the master wax pattern was made and this was used to standardize all the wax patterns of Group B (inlay wax pattern).
Arora A., Yadav A., Upadhyaya V., Jain P, & Verma M. (2018). Comparison of marginal and internal adaptation of copings fabricated from three different fabrication techniques: An in vitro study. The Journal of the Indian Prosthodontic Society, 18(2), 102-107.
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8

3D Printed Dental Pattern Fabrication

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In Group A, the die was scanned using the dental scanner (open technology). Then, the image was transferred to the computer and the Stereolithographic (STL) file generated was used to design the patterns of uniform dimension (0.5 mm). The design file was transferred to the 3D printer (ProJet 1200, 3D SYSTEMS). The 3D printer has a cartridge filled with pattern resin which is added in incremental layers one after another and the patterns were fabricated and immediately invested for casting. For casting of these patterns, conventional protocol was followed as used for fabrication of Group B samples.
Arora A., Yadav A., Upadhyaya V., Jain P, & Verma M. (2018). Comparison of marginal and internal adaptation of copings fabricated from three different fabrication techniques: An in vitro study. The Journal of the Indian Prosthodontic Society, 18(2), 102-107.
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