Finely milled wood powder from all transgenic (3–5 per line) and WT (33) trees was used for monosugar analysis of the greenhouse-grown trees. Extractives were removed with 80% ethanol in HEPES buffer (4 mM, pH 7.5) for 30 min at 95 °C, followed by methanol:chloroform (1:1) extraction and two washes with acetone. To remove starch, the extractive-free wood powder was treated overnight at 37 °C with α-amylase from pig pancreas (Roche 10102814001; 100 units per 100 mg of wood) in potassium phosphate buffer (0.1 M, pH 7.0). For the quantification of monosugars, 0.5 mg of amylase-treated extractive-free wood was methanolyzed using 2 M HCl/MeOH [at 85 °C for 24 h; inositol (10 μg) as internal standard] followed by trisil reagent (1,1,1,3,3,3-hexamethyldisilazane + trimethylchlorosilane + pyridine, 3:1:9) derivatization using the Sylon HTP kit (Supelco; Sigma Aldrich) [53 ]. The monosugars were separated on a J&W DB-5MS column (30 m length, 0.25 mm diameter, 0.25 μm film thickness) (Agilent Technologies) and determined using GC–MS (7890A/5975C; Agilent Technologies).
J w db 5ms column
Manufactured by Agilent Technologies
Sourced in United States
The J&W DB-5MS column is a capillary column used in gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. It features a 5% phenyl-95% dimethylpolysiloxane stationary phase, which provides excellent separation of a wide range of analytes, including non-polar and moderately polar compounds.
Automatically generated - may contain errors
Lab products found in correlation
5975c inert msd, by Agilent Technologies (2 mentions)
7890a 5975c gc ms, by Agilent Technologies (1 mentions)
Sylon htp kit, by Merck Group (1 mentions)
5975c msd, by Agilent Technologies (1 mentions)
5973 mass selective detector, by Agilent Technologies (1 mentions)
Aviii400, by Bruker (1 mentions)
7890a gas chromatography, by Agilent Technologies (1 mentions)
Hp innowax, by Agilent Technologies (1 mentions)
11 protocols using j w db 5ms column
1
Monosaccharide Analysis of Transgenic Wood
2
GC-MS Analysis of Non-Polar Compounds
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For GC−MS, a modified sample preparation protocol by Pedneault et al. (2007) was used [33 (link)]. GC Agilent Technologies 7890A, MS 5975C (Palo Alto, CA, USA) was used for the analysis of non-polar compounds extracted with hexane:diethyl ether (3:1, v/v). One µL of extract was injected in split mode (1:12) into a system equipped with an Agilent J&W DB-5MS column (Palo Alto, CA, USA). The separation was performed using a temperature gradient program, starting at a temperature of 60 °C, which then increased to 280 °C in 3 °C increments. Mass spectra were collected in TIC mode.
3
Metabolic Profiling of Hepatocellular Carcinoma
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The third dataset was collected by Ranjbar et al. and is publicly available in the Metabolights repository [26 (link)] at https://www.ebi.ac.uk/metabolights/MTBLS105 . The study evaluates changes in metabolite levels in hepatocellular carcinoma (HCC) cases vs. patients with liver cirrhosis by analysis of human blood plasma using GC-MS [20 (link)]. Briefly, data was collected using a GC-qMS (Agilent 5975C MSD coupled to an Agilent 7890A GC) equipped with an Agilent J&W DB-5MS column (30 m × 0.25 mm × 0.25 µm film, 95% dimethyl/5% diphenyl polysiloxane) with a 10 m Duragard Capillary column with a 10 min analysis, using a temperature gradient from 60 °C to 325 °C. Only 89 files generated in the selected ion monitoring (SIM) mode were used for validation purposes here. Although SIM normally simplifies peak detection, in this dataset, there were often several peaks detected for the same m/z, meaning that there was still a peak detection issue to be addressed.
4
GC/MS Analysis of VEA Decomposition Products
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VEA decomposition products were identified and quantified using GC/MS (Agilent 6890N GC and 5975C inert MSD equipped with an EI ion source) analysis. Large molecular weight and non-polar degradation products were analyzed by directly injecting 2 μL of sample into an Agilent J&W DB-5MS column (30 m × 0.25 mm i.d., 0.25 μm film) for separation. A solvent delay of 6 min was used; the GC was initially set to 60°C for 1 min, then ramped to 150°C at a rate of 3°C min-1, held at 150°C for 2 min, ramped to 310°C at a rate of 20°C min-1, and then held at 310°C for 5 min. Smaller molecular weight, polar degradation products were analyzed by directly injecting 2 μL of sample into a Rtx-VMS fused silica column (30 m × 0.25 mm i.d., 1.4 μm film). A solvent delay of 6 min was used. The GC was set to 35°C for 1 min, ramped to 240°C at a rate of 10°C min-1, and held 4 min. The detailed procedures for the operation of GC/MS can be found in a previous publication [25 (link)].
5
Characterization of Bacterial Metabolites by NMR and GC-MS
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Nuclear magnetic resonance (NMR) data were collected at 9.4 or 14.1 T by a Bruker AVIII 400 or 600 MHz spectrometer, respectively. The AVIII 400 data were collected using a 5-mm BBFO+ probe. The AVIII 600 was equipped with a 1H/31P/13C/15N/D 5 mm QCI-P cryoprobe. Gas chromatography–mass spectrometry (GC–MS) in electron ionization mode (70 eV) was performed on dichloromethane solutions of BMNs on an Agilent Technologies 6890 GC interfaced to a 5973 mass selective detector from the same manufacturer (Cheshire, UK). The average linear retention index (LRI) was recorded (n=3) using the 25 m Agilent Technologies J&W DB-5 MS column (0.2 mm internal diameter, 0.33 μm film thickness) by reference to an alkane test mix (C7–C30) with the initial temperature set to 40°C (hold 5 min) and programmed to rise to 280°C at 10°C min−1 (hold 15 min). Helium carrier gas was used in constant flow mode (1 ml min−1). A 1-μl splitless injection was performed at 280°C. Mass-to-charge ratios (m/z) of the 10 most abundant ions (% abundance) are listed.
6
GC-MS Analysis of Fatty Acids
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Qualitative and quantitative analysis of the products were performed by GC-MS29 (link). To detect α-alkenes, an Agilent 7890A gas chromatography equipped with HP-INNOWAX (Agilent Technologies, Inc., cross-linked polyethylene glycerol, Santa Clara, CA, USA; 0.25 µm film thickness, 30 m by 0.25 mm) column was adopted. The column heating program was as follows: the initial temperature of oven was set to 40 °C for 4 min, then increased at a rate of 10 °C/min to 250 °C, and hold for 15 min. The α- and β-hydroxy fatty acids detection was carried out using the Agilent J&W DB-5 MS column (0.25 μm film thickness, 30 m by 0.25 mm). Furthermore, an Agilent 5975C MSD quadrupole mass spectrometer with a scan range from 50 to 500 m/z under electron ionization condition (70 eV) was coupled to the GC. The oven temperature was 50 °C initially and ramped up to 300 °C at the above mentioned rate, then 300 °C for 5 min. Quantification was performed using the corresponding authentic standard compounds and heptadecanoic acid as the internal standard.
7
GC-MS Analysis of Brominated Flame Retardants
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The extracts were quantitatively analyzed using an Agilent 6890 gas chromatograph (Agilent Technologies, Santa Clara, CA, USA). The mass spectrometer was used in negative chemical ionization mode and selected ion monitoring mode. The carrier gas was helium (1.0 mL/min), and the reagent gas was methane (1.0 mL/min). The temperatures of the injector, mass spectrometer source and quadrupole were 290 °C, 150 °C and 150 °C respectively.
The PBBA, PBT, PBEB and HBB were analyzed using a different J&W DB-5 MS column (30 m long, 0.25 mm i.d., 0.1 mm film thickness; Agilent Technologies). The oven temperature program started at 100 °C, which was held for 3 min, then increased at 4 °C/min to 300 °C, which was held for 8 min. The fullscan of PBBA, PBT, PBEB and HBB were analyzed under the temperature program that the oven temperature started at 45 °C, increased at 15 °C/min to 200 °C, then increased at 6 °C/min to 300 °C, which was held for 5 min.
The m/z ratios 485.6 and 487.6 were monitored for PBBA and PBT. The m/z ratios that were monitored for PBEB and HBB were 499.6 and 501.6, 547.6 and 549.6, respectively.
The PBBA, PBT, PBEB and HBB were analyzed using a different J&W DB-5 MS column (30 m long, 0.25 mm i.d., 0.1 mm film thickness; Agilent Technologies). The oven temperature program started at 100 °C, which was held for 3 min, then increased at 4 °C/min to 300 °C, which was held for 8 min. The fullscan of PBBA, PBT, PBEB and HBB were analyzed under the temperature program that the oven temperature started at 45 °C, increased at 15 °C/min to 200 °C, then increased at 6 °C/min to 300 °C, which was held for 5 min.
The m/z ratios 485.6 and 487.6 were monitored for PBBA and PBT. The m/z ratios that were monitored for PBEB and HBB were 499.6 and 501.6, 547.6 and 549.6, respectively.
8
Steam-assisted n-Dodecane Cracking
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The synthesized
catalysts were evaluated for steam-assisted n-dodecane
cracking in a fixed bed reactor (PID Microactivity-Effi reactor) at
atmospheric pressure and a 350 °C reaction temperature. N2 gas with a flow rate of 15 cm3 min–1 was used as the inert gas for the reaction. The ratio of steam to n-dodecane was kept at 1.0 to 9.0, and the weight hourly
space velocity (WHSV) was fixed at 4.5 h–1. The
reactant mixture was fed over the catalyst using HPLC liquid pumps.
The products were cooled to separate the gaseous and liquid products.
The collected liquid products were quantified and analyzed using an
offline GC-MS instrument (Agilent J&W DB, 5 ms column), and the
gaseous products were analyzed using online GC (Agilent GasPro column,
30 m length and 0.32 mm diameter).
catalysts were evaluated for steam-assisted n-dodecane
cracking in a fixed bed reactor (PID Microactivity-Effi reactor) at
atmospheric pressure and a 350 °C reaction temperature. N2 gas with a flow rate of 15 cm3 min–1 was used as the inert gas for the reaction. The ratio of steam to n-dodecane was kept at 1.0 to 9.0, and the weight hourly
space velocity (WHSV) was fixed at 4.5 h–1. The
reactant mixture was fed over the catalyst using HPLC liquid pumps.
The products were cooled to separate the gaseous and liquid products.
The collected liquid products were quantified and analyzed using an
offline GC-MS instrument (Agilent J&W DB, 5 ms column), and the
gaseous products were analyzed using online GC (Agilent GasPro column,
30 m length and 0.32 mm diameter).
9
GC/MS Analysis of Vaping Decomposition Products
10
Quantification of Polar and Nonpolar Compounds via GC/MS
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The samples collected from the tube furnace experiments were analyzed
using GC/MS (Agilent 6890N GC and 5975C inert MSD equipped with an
EI ion source). Helium was used as the carrier gas. To quantify more
polar oxygenated products such as DQ in the collected emissions, 2
μL of each sample was directly injected onto a Restek Rtx-VMS
fused silica column [30 m × 0.25 mm i.d. (inside diameter), 1.4
μm film]. The temperature of the GC started at 35 °C, was
held for 1 min, was ramped to 240 °C at a rate of 10 °C
min–1, and was held for 4 min. To quantify nonpolar
compounds such as VEA and 1-pristene, 1 μL of each sample was
injected onto an Agilent J&W DB-5MS column (30 m × 0.25 mm
i.d., 0.25 μm film). The temperature started at 60 °C,
was held for 1 min, was ramped to 310 °C at a rate of 10 °C
min–1, and was held for 5 min.
using GC/MS (Agilent 6890N GC and 5975C inert MSD equipped with an
EI ion source). Helium was used as the carrier gas. To quantify more
polar oxygenated products such as DQ in the collected emissions, 2
μL of each sample was directly injected onto a Restek Rtx-VMS
fused silica column [30 m × 0.25 mm i.d. (inside diameter), 1.4
μm film]. The temperature of the GC started at 35 °C, was
held for 1 min, was ramped to 240 °C at a rate of 10 °C
min–1, and was held for 4 min. To quantify nonpolar
compounds such as VEA and 1-pristene, 1 μL of each sample was
injected onto an Agilent J&W DB-5MS column (30 m × 0.25 mm
i.d., 0.25 μm film). The temperature started at 60 °C,
was held for 1 min, was ramped to 310 °C at a rate of 10 °C
min–1, and was held for 5 min.
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