293F cell line was obtained from Life Technologies (Carlsbad, CA). P815/MICA, RMA, RMA/RG, ID8 cell lines were described previously [21 (link)–23 (link)]. 293F cell line was cultured in Gibco® FreeStyle 293™ Expression Medium (Life Technologies). ID8 and Bosc23 cell lines were cultured in DMEM with a high glucose concentration (4.5g/L) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals, Lawrenceville, GA), 10mM HEPES, 0.1mM non-essential amino acids, 1mM sodium pyruvate, 100U/mL penicillin, 100ug/mL streptomycin, and 50uM 2-ME. RMA, RMA/RG, P815, P815/MICA, K562, and RPMI8866 cell lines were cultured in RPMI 1640 supplemented with 10% heat-inactive FBS, 10mM HEPES, 0.1mM non-essential amino acids, 1mM sodium pyruvate, 100U/mL penicillin, 100ug/mL streptomycin, and 50uM 2-ME.
293f cell line
Manufactured by Thermo Fisher Scientific
The 293F cell line is a variant of the HEK293 human embryonic kidney cell line, commonly used in recombinant protein expression and viral vector production. The 293F cells are suspension-adapted and optimized for high-density growth in serum-free media, making them suitable for bioreactor-based applications. The core function of the 293F cell line is to serve as a versatile and efficient host for the production of a wide range of recombinant proteins and viral vectors.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Freestyle 293 expression medium, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
293f cells, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
4 protocols using 293f cell line
1
Cell Culture Protocol for Diverse Cell Lines
2
Culturing HEK293T and 293F Cell Lines
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HEK293T cells (ATCC) were cultured in DMEM supplemented with 2 mM L-glutamine (Life Technologies) and 10% fetal calf serum (FCS) (HyClone) (complete medium). The 293T cell line was tested for mycoplasma contamination after receipt from ATCC and before expansion and cryopreservation.
The 293F cells (Life Technologies) were cultured in Freestyle 293 Expression Medium (Gibco). The 293F cell line was tested for mycoplasma contamination after receipt from Life Technologies and before expansion and cryopreservation.
The 293F cells (Life Technologies) were cultured in Freestyle 293 Expression Medium (Gibco). The 293F cell line was tested for mycoplasma contamination after receipt from Life Technologies and before expansion and cryopreservation.
3
Maintenance of Human Renal Cell Carcinoma Lines
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Human renal clear cell carcinoma (RCC) cell lines, SKRC-09 (CAIX positive), SKRC-52 (CAIX positive), and SKRC-59 (CAIX negative), were obtained from Dr. Gerd Ritter (Memorial Sloan-Kettering Cancer Center, New York, NY). These lines were grown in RPMI 1640 Medium (Life Technologies) supplemented with 10 % (v/v) heat-inactivated fetal bovine serum (FBS, Gibco), 100 IU/ml penicillin and 100 μg/ml streptomycin (complete medium) at 37 °C with 5 % CO2. RCC4 and 293 T cells (CRL-11268, ATCC) were grown in DMEM Medium (Life Technologies) supplemented with 10 % FBS, 100 IU/ml penicillin and 100 μg/ml streptomycin. The 293 F cell line (Life Technologies) was propagated and transfections for protein production carried out in 293 FreeStyle serum-free medium (Life Technologies).
4
Cultivation of TZM-bl, 293T, and 293F Cell Lines
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The TZM-bl cell-line was obtained from the AIDS Research and Reference Reagent Program and the 293T cell-line was obtained from Dr. George Shaw (University of Pennsylvania, Philadelphia, PA). The cell-lines were cultured at 37°C (5% CO2) in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated foetal bovine serum (FBS), 50 μg/mL gentamicin (Sigma) and 25 mM HEPES, at confluency monolayers were disrupted with 0.25% trypsin and 1 mM EDTA (Sigma). The 293F cell-line (Life Technologies) was maintained in serum and antibiotic free FreeStyle 293 Expression media at 37°C (10% CO2) in an orbital shaker (130 rpm).
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