Mouse anti claudin 4 mab

MDCK II cells were provided by Dr. Masayuki Murata [9 (link)]. Cells were grown in DMEM (high glucose) supplemented with 5% fetal bovine serum. Mouse anti-ZO-1 monoclonal antibody (mAb) (T8/754), rat anti-occludin mAb (MOC37), rabbit anti-claudin-2 polyclonal antibody (pAb), and rabbit anti-claudin-4 pAb were characterized as described previously [27 (link)–30 (link)]. Rabbit anti-claudin-1 pAb (51–9000), mouse anti-claudin-2 mAb (32–5600), rabbit anti-claudin-3 pAb (34–1700), mouse anti-claudin-4 mAb (32–9400), rabbit anti-claudin-7 pAb (34–9100), and alexa fluor 488 phalloidin (A12379) were purchased from Invitrogen. Rabbit anti-nonmuscle myosin heavy chain II-B (MHC-B) pAb (PRB-445P) was purchased from Covance. Mouse anti-E-cadherin mAb (ECCD-2; M108) was purchased from Clontech. Fluorescein isothiocyanate-dextran (FITC-dextran), bumetanide (B3023) and NPPB (N4779) were purchased from Sigma-Aldrich.

Rabbit anti-claudin-1 polyclonal antibody (pAb), mouse anti-claudin-2 monoclonal antibody (mAb), rabbit anti-claudin-3 pAb, mouse anti-claudin-4 mAb, mouse anti-claudin-7 mAb, mouse anti-occludin mAb, rabbit anti-tricellulin pAb, rabbit anti-ZO-1 pAb, rabbit anti-ZO-2 pAb, and rabbit anti-JAM-A pAb were purchased from Invitrogen (Carlsbad, CA, USA). Rabbit anti-claudin-5 pAb and mouse anti-β-actin mAb were purchased from Abcam (Cambridge, MA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Rabbit anti-ATR pAb, anti-ATR pS428 pAb, mouse anti-Chk1 mAb, and rabbit anti-Chk1 pS345 pAb were purchased from Cell Signaling Technology (Beverly, MA, USA). Goat anti-Rabbit IgG peroxidase-conjugated antibody and goat anti-mouse IgG peroxidase-conjugated antibody were purchased from Millipore (Bedford, MA, USA). Alexa Fluor 488 goat anti-rabbit IgG was purchased from Molecular Probes (Eugene, OR, USA).

The following antibodies were used: mouse anti‐p63 monoclonal antibody (mAb) (clone: 4A4; Abcam), mouse anti‐ΔNp63 mAb (clone: BC28; Biocare Medical), mouse anti‐β‐actin mAb (clone: AC‐15; Sigma‐Aldrich), mouse anti‐claudin‐4 mAb (clone: 3E2C1; Invitrogen), mouse anti‐filaggrin mAb (clone: AKH1; Santa Cruz Biotechnology), mouse anti‐caspase‐14 mAb (clone: EPR12927; Abcam), mouse anti‐Ki67 mAb (clone MIB‐1; Dako), rabbit anti‐claudin‐1 mAb (clone: EPRR18871; Abcam), and rabbit anti‐IL‐33 mAb (clone: EPR20417; Abcam). Peroxidase‐conjugated goat anti‐mouse and anti‐rabbit IgGs were purchased from Kirkegaard & Perry Lab Inc.