Cell proliferation was measured using CCK-8 kits (CA1210-100, Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). Cells at the logarithmic phase of growth were seeded in a 96-well plate at a density of 5 × 103 cells per well, and cultured for 3 days. Each well was supplemented with 10 μL of CCK-8 solution and placed in an incubator for 2 h. Subsequently, the absorbance values at 450 nm were measured with a microplate reader (BIO-RAD 680, Bio-Rad, Hercules, CA, US) and the optical density (OD) values were recorded at the 24 h, 48 h and 72 h time intervals, respectively, after which a cell growth curve was plotted.
Cck 8 kit
Manufactured by Solarbio
Sourced in China
The CCK-8 kit is a colorimetric assay used to measure cell viability and cytotoxicity. It contains a water-soluble tetrazolium salt that is reduced by cells to a colored formazan product, which can be quantified using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (16 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Microplate reader, by Bio-Rad (3 mentions)
Penicillin streptomycin, by Solarbio (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Spectramax id5, by Molecular Devices (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Microplate reader, by Bioteck (2 mentions)
Multiwall plate reader, by Agilent Technologies (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Ultraviolet spectrophotometer, by Bio-Rad (1 mentions)
Synergy neo2, by Agilent Technologies (1 mentions)
Ampure xp system, by Agilent Technologies (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Rna nano 6000 assay kit, by Illumina (1 mentions)
3 5 dimethylphenol, by Merck Group (1 mentions)
Bca protein quantitation kit, by Thermo Fisher Scientific (1 mentions)
109 protocols using cck 8 kit
1
Cell Proliferation Assay by CCK-8
2
Quantifying Cell Proliferation via CCK-8
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Cell proliferation was assessed using CCK-8 kits (Solarbio, Beijing, China). After transfection and LPS stimulation, Caco-2 cells were resuspended and split into 96-well plates at 5 × 103 cells/well and incubated with 10 µL of CCK-8 reagent for 2–3 h at 37 °C and 5% CO2 in the dark. Optical density (OD) values were detected at 490 nm using an ultraviolet spectrophotometer (Bio-Rad, USA).
3
Cell Viability Determination Using CCK-8
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Cell viability was determined using CCK-8 kits (Beijing Solarbio Science & Technology, Co., Ltd.). The cells were seeded in 96-well plates (4x103 cells/well) for 0, 24, 48 and 72 h, respectively. Subsequently, CCK-8 solution (10 µl) was added to each well for 2 h, and the OD value was measured at 450 nm.
4
Measuring Cell Proliferation with CCK-8
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CCK-8 kits (Solarbio Biotechnology, Beijing, China) were used to measure Huh7 or HCCLM3 cells proliferation. Cells were seeded at a density of 5 × 103 cells/well into 96-well plates and incubated for 48 h. CCK-8 reagent (10 µl) was added to each well for 1 hr. Miniature microplate readers were used to determine the OD values at 450 nm. Experiments were performed in triplicate.
5
Cell Viability Assessment of BMDMs
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CCK-8 kits (Solarbio, Beijing, China) were used to investigate the cell viability of BMDMs with samples at different concentrations. In brief, BMDMs were cultured with the control, CBMG, VR-CBMG, and QVR-CBMG for 1 day and 3 days, respectively. The cells were then washed and stained with 10% CCK-8 solution for 4 h. The relative cell density was measured using a microplate reader (BioTek Synergy Neo2, Winooski, VT, USA) at an absorbance value of 450 nm.
6
Cytotoxicity and Proliferation Assay
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H2O2 solution, RIPA lysis buffer, 3,5-dimethylphenol (DMSO), and phosphate-buffered saline (PBS) were obtained from Sigma (Shanghai, China). ISO (C16H12O7), SOD, MDA, and proliferation and cytotoxicity assay (CCK-8) kits were obtained from Solarbio (Beijing, China). Dulbecco’s modified Eagle medium (DMEM) and foetal bovine serum (FBS) were purchased from BI Company (Ridgefield CT, USA). Penicillin–streptomycin solution was obtained from Hyclone (Logan, UT, USA). Trypticase was purchased from Gibco (Waltham, MA, USA). Trizol was purchased from Magen; trichloromethane and isopropanol were purchased from Sinopharm; and the RNA Nano 6000 Assay Kit, NEBNext Ultra™ RNA Library Prep Kit for Illumina, and AMPure XP system were from Agilent Technologies (Santa Clara, CA, USA), ABclonal (Woburn, MA, USA), and Beckman Coulter (Brea, CA, USA), respectively. The BCA Protein Quantitation Kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA).
7
Cell Viability Assay using CCK-8
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Cell viability was assessed using CCK-8 kits (SolarBio, CA1210, China). In brief, cells were seeded in 96-well plates (8 × 103 cells/well) for overnight, and 100 μl of medium containing 10 mM CCK-8 was added to each well. After incubating for 2 hours at 37°C, the absorbance at 450 nm was measured using a multimode microplate reader (Enspire, PerkinElmer, Singapore). Calculate cell viability according to the formula in the manual.
8
Apoptosis and ROS Measurement Protocol
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An Acad Bras Cienc (2022) 94(1) e20210314 3 | 14 streptomycinandfetal bovine serum were purchased from Thermo Fisher (USA). Thetrypsin, LPS, and CCK-8 kits were all brought from Beijing Solarbio Company (Beijing, China). The cellular apoptosis and ROS measurement kits were brought from Shanghai YoungChan Company (Shanghai, China). All antibodies were all brought from Abcam company (Cambridge, UK).
9
Cell Viability Assay for LUAD
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The viability of LUAD cells was detected using a CCK8 kit (Solarbio, Beijing, China) following the instructions. Briefly, LUAD cells that seeded in 96-well plates at a density of 1 × 104/well were cultured for 0, 24, 48, 72, and 96 h, respectively. Cells that collected at different time points were then treated with CCK8 reagent for 2 h. The OD at 450 nm was finally measured under a microplate reader (Thermo Fisher Scientific).
10
Curcumin Regulates Gastric Cancer Cell Signaling
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The human gastric cancer cell line, SGC-7901 was obtained from the Laboratory of Pathology, School of Basic Medical, Lanzhou University (Lanzhou, China),31 (link) and the cells were authenticated by STR. Cells were cultured in RPIM-1640 (HyClone, UT, USA) supplemented with 10% fetal bovine serum (FBS; Kibbutz Beit Haemek, Israel) and 1% penicillin/streptomycin (Sigma-Aldrich, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Curcumin and a CCK-8 kit were purchased from Beijing Solarbio Science & Technology (Beijing, China).
Primary antibodies included: Anti-Shh (Abcam, Cambridge, UK), anti-Gli1 antibody (Abcam), anti-Foxm1 antibody (Abcam), anti-β-catenin antibody (Cell Signaling Technology, MA, USA), anti-E-Cadherin antibody (Cell Signaling Technology), anti-vimentin antibody (Cell Signaling Technology), anti-F-actin antibody (Abcam) and anti-β-actin antibody (Thermo Fisher Scientific, MA, USA). Secondary antibodies included: HRP-labeled goat anti-rabbit IgG (Abcam) and HRP-labeled goat anti-mouse IgG (Abcam). All the primary antibodies were diluted to 1:1000. The secondary antibodies were diluted to 1:5000.
Primary antibodies included: Anti-Shh (Abcam, Cambridge, UK), anti-Gli1 antibody (Abcam), anti-Foxm1 antibody (Abcam), anti-β-catenin antibody (Cell Signaling Technology, MA, USA), anti-E-Cadherin antibody (Cell Signaling Technology), anti-vimentin antibody (Cell Signaling Technology), anti-F-actin antibody (Abcam) and anti-β-actin antibody (Thermo Fisher Scientific, MA, USA). Secondary antibodies included: HRP-labeled goat anti-rabbit IgG (Abcam) and HRP-labeled goat anti-mouse IgG (Abcam). All the primary antibodies were diluted to 1:1000. The secondary antibodies were diluted to 1:5000.
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