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31 protocols using nah2po4 2h2o

1

Magnesium Hydroxide from Phosphate Tailings

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H2SO4, H2O2, NH3·H2O, ethanol, methanol, Zn(NO3)2·6H2O, 2-methylimidazole, NaH2PO4·2H2O, NaOH and N,N-dimethylformamide (DMF) were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Thermoplastic polyurethane (TPU) pellets (1180A) were purchased from BASF Company (Germany). The phosphate tailings were obtained from Yichang Tiaoshuihe Phosphate Mine. XRF was carried out to analyze its chemical components. Table 1 shows that phosphorus tailings are mainly composed of CaO (62.05%) and MgO (22.92%). The high content of magnesium oxide indicates that the phosphorus tailings are suitable for preparation of magnesium hydroxide as magnesium sources.
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2

Reagent Procurement for Cell Culture

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IND (analytically pure grade; 98%) was obtained from Macklin, Shanghai, China. SOD was obtained from Solarbio (Beijing, China). RPMI 1640, streptomycin/penicillin, and fetal bovine serum were all obtained from Thermo Scientific Co., Ltd. (Waltham, MA, USA). Na2HPO4·12H2O and NaH2PO4·2H2O were extracted by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). All the chemicals were of analytical grade. All experiments used ultrapure H2O (18.25 MΩ cm) from the Millipore Milli-Q Water Purification System (Billerica, MA, USA). All the kits were purchased from Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China).
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3

Intracellular Oxidative Stress Analysis

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ARS and CAT from bovine liver were purchased from Beijing Solarbio Ltd. Na2HPO4·12H2O and NaH2PO4·2H2O were purchased from Tianjin Damao Chemical Reagent Factory. Phosphate buffered solution (PBS, pH 7.2–7.4) for intracellular oxidative stress analysis was from Beijing Macgene Ltd., Beijing, China. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, and penicillin/streptomycin were all purchased from Thermo Fisher Scientific, Waltham, MA, USA. Dimethyl sulfoxide (DMSO) was provided by from Biofroxx (Germany). Hydrogen peroxide was bought from Sinopharm Chemical Reagent Co., Ltd. Phosphate buffer (a mixture of Na2HPO4·12H2O and NaH2PO4·2H2O solution, pH 7.4) was chosen to control the solution pH. All the water solutions involved in this work were prepared by using ultrapure water, and stock solutions were preserved at 0–4 °C.
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4

Preparation of Selenium Compounds

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L-selenomethionine and L-methionine were obtained from Sigma-Aldrich (St. Louis, MO, USA). NaH2PO4·2H2O, K2HPO4·3H2O and methanol (analytical grade) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). D2O and 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) were obtained from Cambridge Isotope Laboratories (Cambridge, MA, USA).
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5

Steviol Anticancer Properties Evaluation

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Steviol (99% of purity, HPLC) was purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). 5-Fluorouracil (5-FU), dimethyl sulfoxide (DMSO), Na2CO3, NaHCO3, NaCl, KCl, Na2HPO4·12H2O, NaH2PO4·2H2O, EDTA disodium, dodecyl sodium sulfate (SDS), glycine, bromoxylenol blue, ammonium persulphate, tris (hydroxymethyl) methyl amino methane, ponceau, N,N,N, N-tetramethylethylenediamine (TEMED), xylene brilliant cyanin G (BS, G250), and phenylmethylsulfonyl fluoride (PMSF) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Trypsin-EDTA solution, propidium iodide (PI), triton X-100, endonuclease (RNase A), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), penicillin-streptomycin solution (100X), bovine albumin (BSA), BeyoECL Plus, polyvinylidene fluoride, RIPA lysis buffer, and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyasix iodide (JC-1) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). DMEM medium, fetal bovine serum were purchased from Gibco Life Technologies Corporation (Carlsbad, CA, USA). Primary antibodies against p21, p53, cyclin D1, Bax, Bcl-2, caspase 3, β-actin, and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signal Technologies Inc. (Beverly, MA, USA). All other reagents were of analytical grade and used as received unless otherwise stated.
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6

NMR Analysis of Tissue Extracts

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Dulbecco’s modified Eagle’s medium (DMEM) and trypsin were purchased from Thermo Fisher Scientific Inc. (Beijing, China). Fetal bovine serum (FBS) was obtained from Hangzhou Sijiqing Company. Sodium penicillin and streptomycin were purchased from North China Pharmaceutical Company (Hebei, China). NaH2PO4 · 2H2O, K2HPO4 · 3H2O, K2CO3 and methanol (all in analytical grade) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Deuterium oxide (D2O, 99.9%D) and acetyl chloride were obtained from Sigma-Aldrich, Inc. (St. Louis, MO) and sodium 3-(trimethylsilyl) [2,2,3,3-2H4] propionate (TSP) were obtained from Cambridge Isotope Laboratories, Inc. (Miami, U.S.A.). Phosphate buffer was prepared from NaH2PO4 and K2HPO4 with good low-temperature stability40 (link) containing 0.001% TSP (w/v) and 80% D2O (0.15 M, pH 7.45) and employed as solvent for NMR analysis of tissue extracts.
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7

Magnesium-Incorporated Titanium Alloy Coatings

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The titanium sheets (20×20×1 mm and 10×10×1 mm) used in the experiment were cut from commercial TC4 titanium alloy. The cut sample was sequentially cleaned in acetone, ethanol, and distilled water in an ultrasonic cleaner.
Then, the samples were micro-arc-oxidized in an electrolyte solution composed of 17.6 g/L calcium acetate monohydrate (Ca(CH3COO)2·H2O, Sinopharm, Shanghai, China) and 4.74 g/L sodium phosphate dibasic dihydrate (NaH2PO4·2H2O, Sinopharm, Shanghai, China) to fabricate a porous surface layer (the MAO coating). The voltage, frequency and time parameters of the pulsed AC power were 420 V, 50 Hz, and 10 min, respectively. To load magnesium into the porous surface layer (the MAO-Mg coating), magnesium acetate (C4H6 MgO4·4H2O, Sinopharm, Shanghai, China, 1 g/L, 2 g/L, 4 g/L, 8 g/L) was added to the electrolyte. The MAO microporous coatings incorporated with different amounts of Mg2+ were named M1, M2, M4, and M8, respectively.
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8

Biochemical and Oxidative Stress Analysis

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Analytical-grade sodium chloride, DMSO, NaN3, NaH2PO4•2H2O, and Na2HPO4•12H2O were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). HPLC-grade CHCl3 and CH3OH were obtained from Merck (Darmstadt, Germany). D2O (99.9% in D) containing sodium 3-(trimethyl-silyl) propionate-2, 2, 3, 3, d4 (TSP) as an internal standard for chemical shift reference was provided by Sigma‒Aldrich (MO, USA). A buffer system containing 0.2 M Na2HPO4/NaH2PO4 in D2O at pH 7.4 was prepared to prevent the pH effect on the chemical shifts of metabolites at different concentrations. The assay kits for the determination of sodium-potassium ATPase (Na-K-ATPase), cholinesterase (AChE) and lactate dehydrogenase (LDH) were purchased from Abcam (USA). The assay kits for dopamine (DA) were purchased from RD, USA, the assay kits for epinephrine (E) and norepinephrine (NE) were from Abnova, Taiwan, 5-hydroxytryptamine (5HT) assay kits were from BioSource, and gamma-aminobutyric acid (GABA) assay kits were from Santa Cruz, USA. The assay kits of superoxide dismutas (SOD), malonyldialdehyde (MDA), and glutathione peroxidase (GPx) were purchased from Cayman, USA.
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9

Colorimetric Protein Sensing Using GNPs

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Collagen (Col), myoglobin (Mb), glucoamylase (Glu), catalse (Cat) were from Aladdin Reagent Co. Ltd (Shanghai, China). Bovine serum albumin (BSA) was purchased from Gibco (Grand Island, USA). Lysozyme (Lys), pepsin (Pep), trypsin (Try), bovine albumin (BA), bovine hemoglobin (BHb), chloroauric acid tetrahydrate (HAuCl4•4H2O), NaOH, NaH2PO4·2H2O, Na2HPO4·12H2O, NaCl, KCl and CaCl2 were obtained from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). All chemicals were used as received without further purification. 96-well plates (Corning 3632) were obtained from Genetimes Technology. The stock solutions of proteins were prepared using 6 mM phosphate buffered saline (PBS, pH 7.4) buffer.
The pH measurements were performed using a PHS-3C pH meter. For all sensing experiments, imagings were acquired with a flatbed scanner (Epson Perfection V300) in 96-well plates. Transmission electron microscopy (TEM) was performed on a Tencai F20 instrument and operated at 200 kV. UV-vis absorption spectra were recorded using a Lambda 950 UV-vis spectrophotometer from Perkin Elmer. Zeta-potential of GNPs was measured using a Microtrac S3500 (Microtrac Inc., USA), and measured at 25 °C. FT-IR spectroscopy was performed using a Nicolet 6700 spectrometer.
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10

Photochemical Removal of Emerging Contaminants

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BPS, PMS, PS, BPA, H2O2, furfuryl alcohol (FFA), thiamphenicol (TAP), lomefloxacin hydrochloride (LOM), enrofloxacin (ENR), and norfloxacin (NOR), were purchased from Aladdin Chemicals Co. (China). Ca(OH)2, NaCl, NaH2PO4·2H2O, NaNO3, NaHCO3, NaOH, H2SO4, Na2SO4, ethanol (EtOH), tert-butyl alcohol (TBA), ascorbic acid (AA) and l-histidine (l-his), nitro blue tetrazolium (NBT) and p-benzoquinone (BQ) were obtained from Sinopharm (China). Humic acid (HA) was purchased from Xiya Reagent Co. (China). Methanol (EtOH) was supplied by CNW Technologies GmbH (Germany). All chemical reagents were at least of analytical grade or higher purity. Ultrapure water with a resistivity of 18.2 MΩ cm−1 produced from a water purification system was used to prepare all solutions.
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