Four actinobacterial strains were selected for extracts generation following phylogenetic analysis. A volume of 5 mL SV2 media (for 1 L, add 1 g calcium carbonate (Sigma-Aldrich, St. Louis, MO, USA), 15 g glucose (1st BASE, Singapore), 15 g glycerol (VWR, Radnor, PA, USA), and 15 g soya peptone (Oxoid, Hampshire, UK), pH adjusted to 7.0) was used to culture strains at 28 °C for 3 days under constant agitation at 200 rpm to generate a seed culture. The seed cultures were then inoculated in a 1:20 volume into five in-house liquid media (CA02LB, CA07LB, CA08LB, CA09LB, and CA10LB) as shown in Table 5 . These media have been formulated and optimized by the Natural Product Library group at SIFBI for actinobacteria secondary metabolites production. The cultures were incubated for 9 days at 28 °C in the dark with shaking at 200 rpm. Following incubation, the cultures were lyophilized. The dried cultures underwent extraction using methanol (MeOH) and were subsequently filtered through Whatman Grade 4 filter paper. MeOH was then evaporated under reduced pressure to generate the crude extract.
Calcium carbonate
Manufactured by Merck Group
Sourced in Germany, United States, United Kingdom, Spain, India
Calcium carbonate is a white, crystalline solid compound that is the main component of limestone, chalk, and seashells. It is a naturally occurring mineral with the chemical formula CaCO3. Calcium carbonate serves as an effective pH buffer and is commonly used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (12 mentions)
Ethanol, by Merck Group (10 mentions)
Sodium hydroxide (naoh), by Merck Group (10 mentions)
Hydrochloric acid (hcl), by Merck Group (9 mentions)
Sodium chloride (nacl), by Merck Group (9 mentions)
Sodium alginate, by Merck Group (8 mentions)
Glucose, by Merck Group (6 mentions)
Sulfuric acid, by Merck Group (5 mentions)
Sodium bicarbonate, by Merck Group (5 mentions)
Ammonium chloride, by Merck Group (5 mentions)
Acetonitrile, by Merck Group (5 mentions)
Acetic acid, by Merck Group (5 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (5 mentions)
Gallic acid, by Merck Group (4 mentions)
Quercetin, by Merck Group (4 mentions)
Calcium chloride, by Merck Group (4 mentions)
Formic acid, by Merck Group (4 mentions)
Soya peptone, by Thermo Fisher Scientific (3 mentions)
Glycerol, by Avantor (3 mentions)
Sodium carbonate, by Merck Group (3 mentions)
96 protocols using calcium carbonate
1
Actinobacteria Metabolites Production Protocol
2
Preparation of Polymeric Nanoparticles for Drug Delivery
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Acrylamide (A9099, ≥99%), N,N′-bis(acryloyl)cystamine (A4929), ammonium persulfate (A3678, ≥98%), N,N,N′,N′-tetramethylethylenediamine (T9281, 99%), sodium alginate (A2033, medium viscosity), calcium sulfate (C3771, ≥99%), methyl blue (M6900), barium sulfate (11844), L-GSH reduced (≥98%), EDTA (≥99%), DMSO (D8418), sodium bicarbonate (NaHCO3, S5761), calcium carbonate (CaCO3, ≥99%), and phosphate-buffered saline (PBS, pH 7.4) were available commercially from Sigma-Aldrich and used as received unless otherwise noted. Insulin was kindly provided by Novo Nordisk and labeled by Alexa-Fluor® 488. Lumefantrine and rifampicin were purchased from Hangzhou Hysen Pharma Co., Ltd in China. Nanopure water (18 MΩ cm) was acquired by means of a Milli-Q water filtration system, Millipore (St. Charles). SGF (pH ~1.2) was made by dissolving 2 g NaCl and 8.3 ml concentrated HCL in nanopure water and adjusting to 1000 ml.
3
Investigating Vascular Calcification Mechanisms
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Lanthanum hydroxide (Laboratory synthesis), Adenine (Sigma-Aldrich, Cat. #V900471, United States), Lanthanum carbonate (Sigma-Aldrich, Cat. #325767 United States), Calcium carbonate (Sigma-Aldrich, Cat. #V900138 United States), Human aortic vascular smooth muscle cells (Shanghai Yubo Biological Technology Co., Ltd., Cat. #C740, China), RNA Simple Total RNA Kit (Tiangen Biochemical Technology (Beijing) Co., Ltd., Cat. #DP419, China), ReverTra Ace qPCR RT Kit (ToYoBo Life Science, Cat. #FSQ-101, China), SYBR Green Realtime PCR Master Mix (ToYoBo Life Science, Cat. #QPK-201), Lipopolysaccharide (LPS, Sigma-Aldrich, Cat. #L4391), Lanthanum chloride (LaCl3, Sigma-Aldrich, Cat. #298182), Sodium pyrophosphate tetrabasic decahydrate (PPI, Sigma-Aldrich, Cat. #S6422), Monoclonal Mouse anti-SM22α (SC-53932, Santa Cruz Biotechnology, RRID: AB_1129519 ), Monoclonal Mouse anti-BMP-2/4 (SC-137087, Santa Cruz Biotechnology, RRID: AB_2258985 ), Monoclonal Mouse anti-Runx2 (SC-101145, Santa Cruz Biotechnology, RRID: AB_1128251 ), Polyclonal Rabbit anti-β-Actin (K101527P, Solarbio), Monoclonal Rabbit anti-TRAF6 (ab40675, Abcam, RRID: AB_778573 ), Monoclonal Mouse anti-NF-κB (#6956, Cell Signaling Technology, RRID: AB_10828935 ), Goat anti-Mouse IgG (H + L) (A23910, Abbkine), Goat anti-Rabbit IgG (H + L) (A23920, Abbkine), Polyclonal Rabbit anti-Lamin A (10298-1-AP, Proteintech, RRID:AB_2296961 ).
4
Extraction and Characterization of Phytochemicals
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Dichloromethane, ethanol, hexane, ethyl acetate, anhydrous sodium acetate, copper (II) sulfate pentahydrate, aluminum potassium sulfate dodecahydrate, calcium carbonate, methanol, aluminum chloride, potassium acetate, Folin-Ciocalteau reagent, sodium carbonate, quercetin, and gallic acid were purchased from Sigma Chemical Co. (Philippines). All the chemicals and reagents were of analytical grade for this study.
5
Alendronate Scaffold Fabrication and Characterization
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Alendronate sodium trihydrate was purchased from Tokyo Chemical Industry Co. Ltd. (TCI). Ethylenediamine (EDA; 99.5% purity), calcium carbonate, calcium phosphate, pyrophosphate, calcium oxide, Alizarin Red S, and deuterium oxide were purchased from Sigma Aldrich (St. Louis, MO, USA). All the other reagents were of the analytical grade and used without further purification. Double deionized water used throughout the study was prepared using a Milli-Q system (Millipore, Bedford, MA, USA). The cell fixation and permeabilization solution were purchased from BD (Cytofix/Cytoperm). CDHA scaffolds and normal rat femurs (8 weeks, male, Sprague-Dawley) were supplied by the Korea Institute of Materials Science (KIMS) and Bio-Evaluation Center at KRIBB, respectively.
6
Kevlar Fabric Waste Composite Development
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The Kevlar woven fabric wastes were obtained from VEBA textiles, Hradec Králové, Czech Republic. The Green epoxy resin CHS-Epoxy G520 and hardener TELALIT 0600 were supplied by Spolchemie, nad Labem, Czech Republic. The rest of the auxiliaries Methanol (99.8%) and Calcium carbonate (≥99%) were obtained from Sigma Aldrich. The activated carbon particles were obtained from Anusawari, Bang Khen, Bangkok and Thailand. The 3-(Trimethoxysilyl)propyl methacrylate (Catalog Number M6514) and n-Propyltriethoxysilane were obtained using Merck chemicals, Burlington, MA, USA.
7
Synthesis of PVA-CaCO3-TiO2 Composite
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The polyvinyl alcohol (PVA) with Mw = 125,000 gm/mole, calcium carbonate (CaCO3), titanium dioxide (TiO2) were procured from Sigma-Aldrich, isopropanol, and silver nitrate (AgNO3) were purchased from Merck.
8
Synthesis and Characterization of Organosulfur Compounds
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All chemicals used were of at
least HPLC grade. DMTS, DMDS, calcium carbonate, calcium bicarbonate,
hexane, ethyl acetate (EtOAc), hydrogen peroxide (H2O2), acetone, ethanol, and SMMTS were purchased from Sigma-Aldrich
(St. Louis, MO, USA). mCPBA, acetonitrile, and water
were purchased from Acros Organics (Thermo Fisher Scientific, Waltham,
MA, USA). Poly 80 was obtained from Alfa Aesar (Tewksbury, MA, USA),
and sorbitan oleate 80 (Span 80) was purchased from TCI America (Portland,
OR, USA). Except where other temperatures are noted, all experiments
were carried out in a temperature-controlled laboratory at 22 °C.
least HPLC grade. DMTS, DMDS, calcium carbonate, calcium bicarbonate,
hexane, ethyl acetate (EtOAc), hydrogen peroxide (H2O2), acetone, ethanol, and SMMTS were purchased from Sigma-Aldrich
(St. Louis, MO, USA). mCPBA, acetonitrile, and water
were purchased from Acros Organics (Thermo Fisher Scientific, Waltham,
MA, USA). Poly 80 was obtained from Alfa Aesar (Tewksbury, MA, USA),
and sorbitan oleate 80 (Span 80) was purchased from TCI America (Portland,
OR, USA). Except where other temperatures are noted, all experiments
were carried out in a temperature-controlled laboratory at 22 °C.
9
Haem-Mediated Colon Carcinogenesis Diet
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All diets were based on a modified low calcium AIN76 diet in a powdered form (UPAE, INRA, France), balanced in iron (haem in haem-enriched diets vs. ferric citrate in control diets), proteins (20% using casein), and lipids (5% using safflower oil). Haem groups (HEM and HEM-Ca) received 1.5 μmol/g diet of haemin (Sigma Chemical), and control groups (CON and Ca) received 0.036% of ferric citrate to balance iron level. Calcium level is critical for the haem promotion of carcinogenesis; calcium was thus excluded from mineral mix but dibasic calcium phosphate was included in all diets at a low concentration of 3.4 g/kg. Calcium groups (Ca and HEM-Ca) received 2% of calcium carbonate (Sigma chemical) in order to chelate haem in the intestinal lumen.
10
Synthesis and Characterization of Multicomponent Metal Nitrate Complexes
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All reagents utilized in the experiments were of analytical quality. The reagents included hexamethylenetetramine (HMTA, 99%), potassium chloride (KCl), iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O, ≥98%), ammonium chloride (NH4Cl), zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), lead(II) nitrate (Pb(NO3)2), nitrite ion solution (10 mM), sodium nitrate (NaNO3), ethylene glycol (99.8%), sodium sulfate (Na2SO4), magnesium sulfate (MgSO4), calcium chloride (CaCl2), calcium carbonate (CaCO3), and PBS (phosphate buffered saline, 1.0 M, pH 7.4), all of which were sourced from Sigma-Aldrich (St. Louis, MO, USA). The preparation of all solutions was carried out using Milli-Q water.
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