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Dermo1 cre

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Dermo1-Cre is a Cre recombinase transgenic mouse line that expresses Cre under the control of the Dermo1 promoter. Dermo1 is a transcription factor involved in the development of mesenchymal tissues. This mouse line can be used to conditionally delete or express genes in mesenchymal cells.

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Lab products found in correlation

Prx1 cre, by Jackson ImmunoResearch (3 mentions) Tie2 cre, by Jackson ImmunoResearch (2 mentions) Vav1 cre, by Jackson ImmunoResearch (2 mentions) β actin gfp, by Jackson ImmunoResearch (2 mentions) Tamoxifen solution, by Merck Group (2 mentions) Dermo1 cre mice, by Jackson ImmunoResearch (2 mentions) Ubc creert2, by Jackson ImmunoResearch (1 mentions) Trp53f f, by Jackson ImmunoResearch (1 mentions) Stat1flox, by Jackson ImmunoResearch (1 mentions) Twist2 cre, by Jackson ImmunoResearch (1 mentions) Col2 cre, by Jackson ImmunoResearch (1 mentions) Rosalacz, by Jackson ImmunoResearch (1 mentions) Cag cat egfp, by Jackson ImmunoResearch (1 mentions) Lysm cre, by Jackson ImmunoResearch (1 mentions) Osx cre, by Jackson ImmunoResearch (1 mentions) Ctsk cre, by Jackson ImmunoResearch (1 mentions)

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Dermo1-Cre mice (5 citations) Dermo1-Cre knock-in mice (2 citations)

10 protocols using dermo1 cre

1

Snail1 Regulation of Vascular Development

Cited 3 times
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Mice were housed under standard condition and protocols approved by the University Committee on Use and Care of Animals (UCUCA). Mice carrying Snail1 alleles 17 (link) and mice with Snail1-LacZ knock-in alleles 23 (link) were generated and maintained in our laboratory. Tie2-Cre, Vav1-Cre, Dermo1-Cre and β-actin-GFP transgenic mice were obtained from Jackson Laboratory. VE-cadherin-Cre ERT2 transgenic mice were provided by ML Iruela-Arispe (University of California, Los Angeles) 66 (link). Littermate controls of both sexes were used in all experiments. All mouse strains were backcrossed into the C57BL/6J background for at least 7 generations. To inhibit Notch signaling in vivo, timed pregnant mice were injected subcutaneously with 100 mg/kg DAPT (Tocris Bioscience) dissolved in 10% ethanol and 90% corn oil at E7.5, E8.5 and E9.5 with the embryos dissected at E10.5 6 (link). Gene inactivation in Snail1LacZ/fl;VE-cadherin-Cre-ERT2+ embryos was triggered by i.p. injections of 150 μl of tamoxifen solution (10 mg/ml, dissolved in 1:10 ethanol/corn oil; Sigma) into pregnant females at E11.5 and E13.5. Gene inactivation in pups was triggered by i.p. injections of 50 μl tamoxifen solution (10 mg/ml) at p1 and p3. Eyes were retrieved from pups at p6 and fixed in 4% paraformaldehyde-PBS overnight at 4°C. Retinas were dissected and subjected to whole-mount PECAM-1.
Wu Z.Q., Rowe R.G., Lim K.C., Lin Y., Willis A., Tang Y., Li X.Y., Nor J.E., Maillard I, & Weiss S.J. (2014). A Snail1/Notch1 Signaling Axis Controls Embryonic Vascular Development. Nature communications, 5, 3998.
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2

Genetically Modified Mice for Immune Research

Cited 1 time
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WT, Dermo1-Cre;Myd88fl/fl, Villin-Cre;Myd88fl/fl, and Cd11b-Cre;Myd88fl/fl mice on a C57BL/6 background were bred and kept under specific pathogen-free (SPF) conditions at the University of Michigan Animal Facility. Villin-Cre;Myd88fl/fl and Cd11b-Cre;Myd88fl/fl mice were a gift from Dr. Xiaoxia Li, the Cleveland Clinic (23 , 24 (link)). Myd88fl/fl mice and Dermo1-Cre mice were purchased from Jackson laboratory and crossed to generate mesenchymal-specific MyD88 deficient Dermo1-Cre;Myd88fl/fl mice. All the experimental procedures were performed in accordance with the protocols approved by the University Committee on Use and Care of Animals (UCUCA) at University of Michigan.
Kim D., Seo S.U., Zeng M.Y., Kim W.U., Kamada N., Inohara N, & Núñez G. (2017). Mesenchymal cell-specific MyD88 signaling promotes systemic dissemination of Salmonella Typhimurium via inflammatory monocytes. Journal of immunology (Baltimore, Md. : 1950), 199(4), 1362-1371.
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3

Conditional Gene Knockout Mouse Models

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The Dermo1-Cre, Prx1-Cre, UBC-CreERT2, and Trp53f/f mice were obtained from Jackson Lab and maintained by breeding with WT C57BL/6 strains. The Senp6 floxed mice were created by the laboratory of Dr. Edward Yeh and maintained on C57BL/6 background. Mice at embryonic or perinatal stages were not separated by sexes. The mice at p12, p21, and adult age used in the present study were males. The littermates were randomly grouped based on genotypes for all the experiments. The animal procedures were approved by the Van Andel Research Institute Animal Care and Use Committee.
Li J., Lu D., Dou H., Liu H., Weaver K., Wang W., Li J., Yeh E.T., Williams B.O., Zheng L, & Yang T. (2018). Desumoylase SENP6 maintains osteochondroprogenitor homeostasis by suppressing the p53 pathway. Nature Communications, 9, 143.
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4

Conditional and Global Stat1 Deletion

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All animal experiments were performed according to procedures approved by the Institutional Care and Use Committee of the Oklahoma Medical Research Foundation. Mice were maintained in a 12 hr light/dark cycle and housed in groups of two to five with unlimited access to food and water. All strains were maintained on a mixed C57BL6/129 genetic background at room temperature. Both males and females were analyzed. All animal comparisons were age-matched, and littermate controls were used whenever possible. For conditional Stat1 deletion, the lines Stat1flox (JAX:012901), Twist2Cre also known as Dermo1Cre (JAX:008712), Apoe−/− (JAX:002052), and Myh11-CreERtg (JAX:019079) were purchased from the Jackson Laboratories. For global Stat1 deletion, Stat1floxed was crossed with the germline deleter strain Sox2-Cretg (JAX:008454) to generate the Stat1-null allele, followed by intercrossing to generate Stat1+/+, Stat1+/−, and Stat1−/− genotypes and to eliminate Sox2-Cretg.
Medley S.C., Rathnakar B.H., Georgescu C., Wren J.D, & Olson L.E. (2020). Fibroblast-specific Stat1 deletion enhances the myofibroblast phenotype during tissue repair. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 28(4), 448-459.
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5

Genetic Mouse Models for Tissue Development

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Mouse work was carried out following the recommendations from the National Research Council Guide for the Care and Use of Laboratory Animals, with the protocols approved by the Institutional Animal Care and Use Committee of Shanghai, China (SYXK [SH] 2011‐0112). Dermo1‐Cre, Col2‐Cre, and ROSA‐tdTomato mouse lines were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Rosa‐STOP‐DTR mice were generated in Waisman's laboratory and floxed HB‐EGF mouse was generated from Mekada's laboratory. These mice were kept at the SPF facility of Shanghai Jiao Tong University. HB‐EGFf/f mice were crossed with Dermo1‐Cre mice to generate Dermo1‐Cre; HB‐EGFf/f mice. Dermo1‐Cre and Col2‐Cre mice were crossed with Rosa‐STOP‐DTR mice to generate Dermo1‐HB‐EGF mice and Col2‐HB‐EGF mice, respectively. Dermo1‐Cre mice were crossed with ROSA‐tdTomato mice to generate Dermo1‐tdTomato mice.
Li P., Deng Q., Liu J., Yan J., Wei Z., Zhang Z., Liu H, & Li B. (2018). Roles for HB‐EGF in Mesenchymal Stromal Cell Proliferation and Differentiation During Skeletal Growth. Journal of Bone and Mineral Research, 34(2), 295-309.
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6

Conditional Knockout Mouse Generation

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Cbfβf/f and Dermo1-Cre mice were purchased from Jackson Laboratory and were crossed to generate Cbfβf/+ Dermo1-Cre mice, which were intercrossed to obtain homozygous CKO (Cbfβf/f Dermo1-Cre) mice. The genotypes of the mice were determined by PCR. All mice were maintained under a 12-hour light–dark cycle with ad libitum access to regular food and water at the University of Alabama at Birmingham (UAB) Animal Facility. The study was approved by the UAB Institutional Animal Care and Use Committee and conformed to National Institutes of Health (NIH) guidelines.
Wu M., Li C., Zhu G., Wang Y., Jules J., Lu Y., McConnell M., Wang Y.J., Shao J.Z., Li Y.P, & Chen W. (2014). Deletion of Core-binding factor β (Cbfβ) in mesenchymal progenitor cells provides new insights into Cbfβ/Runxs complex function in cartilage and bone development. Bone, 65, 49-59.
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7

Mouse Line Generation and Validation

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The p38αf/f mouse line was generated in Dr. Yibin Wang's laboratory at UCLA. The Dermo1-Cre, Prx1-Cre, Rosa-LacZ, and Rosa-tdTamato mouse lines were purchased from The Jackson Laboratories. These mice were housed in a pathogen-free facility at the Bio-X Institutes at Shanghai Jiao Tong University and the experimental protocol was approved by the Animal Welfare Committee of the University.
Cong Q., Jia H., Biswas S., Li P., Qiu S., Deng Q., Guo X., Ma G., Ling Chau J.F., Wang Y., Zhang Z.L., Jiang X., Liu H, & Li B. (2016). p38α MAPK Regulates Lineage Commitment and OPG Synthesis of Bone Marrow Stromal Cells to Prevent Bone Loss under Physiological and Pathological Conditions. Stem Cell Reports, 6(4), 566-578.
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8

Conditional Emc3 Deletion in Mesenchymal Cells

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Mice were housed in pathogen-free conditions according to the protocols approved by the Institutional Animal Care and Use Committee at Cincinnati Children’s Hospital Research Foundation. Conditional Emc3fl mice were generated as described.15 (link) For mesenchyme-specific deletion of Emc3, Emc3fl/fl mice were bred with Dermo1-Cre27 (link)(Jackson Labs, stock number 008712). To label and track Cre-mediated recombination, CAG-CAT-EGFP (Jackson Labs, stock number 024636) mice were used to generate CAG-CAT-EGFP31 (link); Emc3fl/fl mice and bred with Dermo1-Cre; Emc3fl/+ mice. 2–4 month- females and 2–6 month-males were used for breeding. For fetal studies, mice were mated overnight, and the presence of a vaginal plug was defined as E0.5. Both male and female embryos were collected and examined. No discrepancy of phenotypes was observed between genders.
Tang X., Wei W., Snowball J.M., Nakayasu E.S., Bell S.M., Ansong C., Lin X, & Whitsett J.A. (2022). EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly. iScience, 26(1), 105667.
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9

Transgenic Mouse Models for Bone Research

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Animal work was carried out following the recommendations from the National Research Council Guide for the Care and Use of Laboratory Animals, with the protocols approved by the Institutional Animal Care and Use Committee of Shanghai, China (SYXK(SH)2011-0112). The Prx1-Cre, Dermo1-Cre, Osx-Cre, LysM-Cre, Ctsk-Cre, and floxed Tsc1 mouse lines were purchased from The Jackson Laboratory. For micro-CT and histomorphometry analysis, male mice at age 2.5 months were used, with the number of mice shown for each figure. For other mouse experiments, three mice (2.5-month-old male or female) were used.
Wu H., Wu Z., Li P., Cong Q., Chen R., Xu W., Biswas S., Liu H., Xia X., Li S., Hu W., Zhang Z., Habib S.L., Zhang L., Zou J., Zhang H., Zhang W, & Li B. (2017). Bone Size and Quality Regulation: Concerted Actions of mTOR in Mesenchymal Stromal Cells and Osteoclasts. Stem Cell Reports, 8(6), 1600-1616.
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10

Snail1 Regulation of Vascular Development

Cited 3 times
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Mice were housed under standard condition and protocols approved by the University Committee on Use and Care of Animals (UCUCA). Mice carrying Snail1 alleles 17 (link) and mice with Snail1-LacZ knock-in alleles 23 (link) were generated and maintained in our laboratory. Tie2-Cre, Vav1-Cre, Dermo1-Cre and β-actin-GFP transgenic mice were obtained from Jackson Laboratory. VE-cadherin-Cre ERT2 transgenic mice were provided by ML Iruela-Arispe (University of California, Los Angeles) 66 (link). Littermate controls of both sexes were used in all experiments. All mouse strains were backcrossed into the C57BL/6J background for at least 7 generations. To inhibit Notch signaling in vivo, timed pregnant mice were injected subcutaneously with 100 mg/kg DAPT (Tocris Bioscience) dissolved in 10% ethanol and 90% corn oil at E7.5, E8.5 and E9.5 with the embryos dissected at E10.5 6 (link). Gene inactivation in Snail1LacZ/fl;VE-cadherin-Cre-ERT2+ embryos was triggered by i.p. injections of 150 μl of tamoxifen solution (10 mg/ml, dissolved in 1:10 ethanol/corn oil; Sigma) into pregnant females at E11.5 and E13.5. Gene inactivation in pups was triggered by i.p. injections of 50 μl tamoxifen solution (10 mg/ml) at p1 and p3. Eyes were retrieved from pups at p6 and fixed in 4% paraformaldehyde-PBS overnight at 4°C. Retinas were dissected and subjected to whole-mount PECAM-1.
Wu Z.Q., Rowe R.G., Lim K.C., Lin Y., Willis A., Tang Y., Li X.Y., Nor J.E., Maillard I, & Weiss S.J. (2014). A Snail1/Notch1 Signaling Axis Controls Embryonic Vascular Development. Nature communications, 5, 3998.
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