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Alexa 488 green conjugated anti rabbit igg

Manufactured by Thermo Fisher Scientific
Sourced in United States

Alexa Fluor 488 (green)-conjugated anti-rabbit IgG is a secondary antibody used to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications. It is a fluorescent-labeled antibody that binds to the Fc region of rabbit immunoglobulin G (IgG) molecules, allowing for the indirect detection of target antigens recognized by rabbit primary antibodies.

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4 protocols using alexa 488 green conjugated anti rabbit igg

1

Molecular Mechanisms of Cell Signaling Regulation

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A rabbit polyclonal anti-actin antibody, EGFR inhibitor (AG1478) and 2-deoxy-D-glucose (2-DG) were obtained from Sigma-Aldrich (St. Louis, Mo., USA). Dobutamine hydrochloride was obtained from Wako Laboratory Chemicals (Tokyo, Japan). Recombinant human amphiregulin was obtained from PeproTech (Rocky Hill, NJ). Rabbit polyclonal anti-lipolysis-stimulated receptor (LSR) and anti-tricellulin (TRIC) antibodies were obtained from Zymed Laboratories (San Francisco, CA). A mouse monoclonal anti-LSR antibody was obtained from Abnova (Yaipei, Taiwan). A rabbit polyclonal anti-angiomotin (AMOT) antibody was obtained from Bioss, Inc. (Woburn, MA). Rabbit monoclonal anti-Merlin, anti-phospho- YAP (ser127) and anti-phospho-AMPK (ther172) antibodies were obtained from Cell Signaling Technology Japan (Tokyo, Japan). A rabbit polyclonal anti-YAP antibody was obtained from Novus Biologicals (Littleton, CO). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were purchased from Molecular Probes, Inc. (Eugene, OR).
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2

Investigating Cell Signaling Pathways

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A mouse monoclonal anti-cytokeratin 7 antibody, rabbit polyclonal anti-actin antibody and JAK2/STAT inhibitor (AG490) were obtained from Sigma-Aldrich (St. Louis, Mo., USA). Bromodeoxyuridine (BrdU) was obtained from Thermo Fisher Scientific (Waltham, MA). An anti-BrdU antibody was obtained from Dako (Tokyo, Japan). A rabbit polyclonal anti-vimentin (H-84) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A PI3K inhibitor (LY294002) and mitogen-activated protein kinase (MAPK) inhibitor (U0126) were purchased from Calbiochem-Novabiochem Corporation (San Diego, CA). A mouse monoclonal anti-β-catenin antibody was obtained from BD Transduction Laboratories (San Jose, CA). Rabbit polyclonal anti-lipolysis-stimulated receptor (LSR) and anti-tricellulin (TRIC) antibodies were obtained from Zymed Laboratories (San Francisco, CA). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were purchased from Molecular Probes, Inc. (Eugene, OR). Recombinant human leptin and recombinant human adiponectin were obtained from PeproTech (Rocky Hill, NJ). Metformin was purchased from Wako (Tokyo, Japan). Berberine was obtained from Tokyo Chemical Industry, Inc. (Tokyo, Japan).
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3

Protein Localization and Acetylation Analysis

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Metformin was purchased from Wako (Tokyo, Japan). Trichostatin A (TSA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). HDAC6 inhibitor was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit polyclonal anti-LSR antibodies were obtained from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-TRIC and ZO-1 antibodies were obtained from Zymed Laboratories (San Francisco, CA, USA). Rabbit polyclonal anti-acetylated lysine, anti-phospho-AMPKα (Thr172), and anti-HDAC6 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-acetylated tubulin and polyclonal rabbit anti-actin antibodies were obtained from Sigma-Aldrich. Monoclonal mouse anti-α-tubulin and anti-β-tubulin antibodies were purchased from Wako. Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were acquired from Molecular Probes, Inc. (Eugene, OR, USA). The ECL Western blot system was purchased from GE Healthcare UK, Ltd. (Buckinghamshire, UK).
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4

Antibodies for Tight Junction Analysis

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Rabbit polyclonal anti-tricellulin (TRIC), anti-claudin (CLDN)-1 and -2 antibodies and mouse monoclonal CLDN-2 and anti-occludin (OCLN) antibodies were obtained from Zymed Laboratories (San Francisco, CA, USA). Rabbit polyclonal anti-lipolysis-stimulated lipoprotein receptor (LSR) antibodies were from Novus Biologicals (Littleton, CO, USA). A mouse monoclonal anti-LSR antibody was obtained from Abnova (Taipei, Taiwan). A rabbit polyclonal anti-p63 antibody was obtained from Abcam (Cambridge, UK). Mouse monoclonal anti-acetylated tubulin and antiCK7 antibodies and a rabbit polyclonal anti-actin antibody were from Sigma-Aldrich (St. Louis, MO, USA). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies and Alexa 594 (red)-conjugated phalloidin were from Molecular Probes, Inc. (Eugene, OR, USA). HRP-conjugated polyclonal goat anti-rabbit IgG was from Dako A/S (Glostrup, Denmark). The ECL Western blotting system was from GE Healthcare UK, Ltd. (Buckinghamshire, UK). A rabbit polyclonal anti-actin antibody and EGFR inhibitor (AG1478) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A TGF-β receptor type 1 inhibitor (EW-7197) was obtained from Cayman Chemical (Ann Arbor, MI, USA). FITC-dextran (FD-4, MW 4.0 kDa) was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).
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