Agilent 6230 lc tof mass spectrometer

Chemical reagents for small molecule and peptide synthesis were purchased from various vendors and used as received. The phospholipids were purchased from Avanti Polar Lipids (Alabaster, Al). PBS buffer, DMEM/High glucose media, RPMI 1640 media, and Pen/Strep were purchased from Thermal Scientific (Amarillo, TX). The Gram-positive bacteria (B. subtilis (ATCC 663) and S. aureus (ATCC 6538)) were purchased from Microbiologics (Cloud, MN) as lyophilized cell pellet. E. coli (BL 21) was a gift from the lab of Professor Mary F. Roberts at Boston College. NMR data of the small molecules were collected on a VNMRS 500 MHz NMR spectrometer. MS data were generated by using an Agilent 6230 LC TOF mass spectrometer. Peptide synthesis was carried out on a Tribute peptide synthesizer from Protein Technologies. The fluorescence anisotropy experiments were performed by using a SpectraMax M5 plate reader. Fluorescence images were taken on a Zeiss Axio Observer A1 inverted microscope. Confocal images were taken on the Leica SP5 confocal fluorescence microscope housed in the Biology Department of Boston College. Flow cytometry analyses were carried out on a BD FACSAria cell sorter also housed in the Biology Department of Boston College.