1 aminobenzotriazole abt

Manufactured by Merck Group

Sourced in United States, Macao

1-aminobenzotriazole (ABT) is a chemical compound used in laboratory research. It serves as an inhibitor for certain enzymes. The core function of ABT is to block the activity of these specific enzymes, which can be useful in scientific investigations. No further details or interpretations are provided.

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1-Aminobenzotriazole (11 citations) Aminobenzotriazole (2 citations) ABTS TM (2 citations)

7 protocols using 1 aminobenzotriazole abt

Xanthotoxol Cytochrome P450 Inhibition

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Xanthotoxol (purity > 98%) was purchased from Sichuan Weikeqi Biotechnology Co. Ltd. (Sichuan, China). Paclitaxel, 1-aminobenzotriazole (ABT), phenacetin, sulfaphenazole, chlorzoxazone, quinidine, clomethiazole, furafylline, 8-methoxypsoralen, coumarin, diclofenac, quercetin, dextromethorphan, ketoconazole, testosterone, S-mephenytoin, omeprazole, glucose-6-phosphate dehydrogenase, NADP⁺, and D-glucose-6-phosphate were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were the highest purity commercially available or HPLC grade.

Ma Z., Shi X., Zhang G., Guo F., Shan L, & Cai J. (2016). Metabolism and Metabolic Inhibition of Xanthotoxol in Human Liver Microsomes. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 5416509.

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Synthesis of GNE Pyrazole Compounds

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GNE1, N²-(((1r,4r)-4-aminocyclohexyl)methyl)-5-chloro-N⁴-(5-cyclopropyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine; GNE2, N²-(((1r,4r)-4-aminocyclohexyl)methyl)-N⁴-(5-cyclo- propyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine; GNE3, 2-(4-aminopiperidin-1-yl)-N-(5-cyclopropyl-1H-pyrazol-3-yl) thieno [3 (link),2-d]pyrimidin-4-amine; and the N-acetylated metabolite of GNE1 (GNE4), N-((1r,4r)-4-(((5-chloro-4-((5-cyclopropyl-1H-pyrazol-3-yl)amino)pyrimidin-2-yl)amino) methyl)cyclohexyl)acetamide were synthesized in-house (supplemental method). Acetyl coenzyme A (acetyl-CoA) sodium salt, 1-aminobenzotriazole (ABT), bovine serum albumin, ethylenediaminetetraacetic acid (EDTA), indomethacin, ketamine/xylazine HCl solution, Krebs-Henseleit buffer, labetalol, loperamide, and polyethylene glycol (PEG400) were purchased from Sigma-Aldrich (St. Louis, MO). Tetrasodium salt of NADPH was obtained from EMD Millipore (Billerica, MA). All other reagents and solvents of highest quality were purchased from commercial vendors.

Fan P.W., Chen J.Z., Jaochico M.A., La H., Liu N., Mulder T., Cass R.T., Durk M., Messick K., Valle N., Liu S., Lee W., Crawford J.J., Rudolph J., Murray L.J., Khojasteh S.C, & Wright M. (2016). Rate-Determining and Rate-Limiting Steps in the Clearance and Excretion of a Potent and Selective p21-Activated Kinase Inhibitor: A Case Study of Rapid Hepatic Uptake and Slow Elimination in Rat. Drug Metabolism Letters, 10(1), 91-100.

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Evaluating Dioxin Receptor Modulators

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TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxine), skatole (3-methylindole), indole-3-carbinol (I3C), 1-aminobenzotriazole (ABT) and actinomycin D were from Sigma-Aldrich (Saint-Louis, MO, US). CH-223191 (2-Methyl-2H-pyrazole-3-carboxylic acid-(2-methyl-4-o-tolyl-azophenyl)-amide, an antagonist of TCDD-mediated AhR activation [24 (link), 25 (link)], was from Calbiochem (Merck KGaA, Damstadt, Germany).

Rasmussen M.K., Balaguer P., Ekstrand B., Daujat-Chavanieu M, & Gerbal-Chaloin S. (2016). Skatole (3-Methylindole) Is a Partial Aryl Hydrocarbon Receptor Agonist and Induces CYP1A1/2 and CYP1B1 Expression in Primary Human Hepatocytes. PLoS ONE, 11(5), e0154629.

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Protective Effects of ABT and NBDHEX Against OTA-Induced Cytotoxicity in PTECs

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1-Aminobenzotriazole (ABT) was purchased from Sigma-Aldrich (St. Louis, MO). Human PTECs cultured in MPS were checked for cell confluency and tubule morphology before OTA treatment in the presence or absence of ABT or NBDHEX. Kidney MPS with 95–100% confluence was used for further treatment. PTECs cultured in MPS were treated with or without OTA (10 μM) in the presence or absence of ABT (1 mM) or NBDHEX (3 μM) in PTEC culture media for 72 hr at a flow rate of 1.0 μL/min. Concentrations of ABT and NBDHEX were selected based on 2D findings that the LC₅₀ values of NBDHEX and ABT against HK2 cells and PTECs were 4.3 μM and > 0.5 mM, respectively (Figure S4). At the end of treatment, LIVE/DEAD staining was performed to analyze the effect of ABT or NBDHEX on OTA-induced cytotoxicity. Quantitative percent cytotoxicity was calculated as described in the LIVE/DEAD staining section (shown in materials and methods section 2.4).

Imaoka T., Yang J., Wang L., McDonald M.G., Afsharinejad Z., Bammler T.K., Ness K.V., Yeung C.K., Rettie A.E., Himmelfarb J, & Kelly E.J. (2020). Microphysiological System Modeling of Ochratoxin A-Associated Nephrotoxicity. Toxicology, 444, 152582.

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Antihistamine Metabolism Inhibition

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The ranges of concentrations of loratadine and desloratadine are listed in Table 1. The maximum concentration was set at 50 μM according to a previous report (Takagi et al., 2016) . These compounds were initially dissolved in DMSO and diluted in the culture medium to obtain the desired final concentrations. The final DMSO concentration in the culture medium was 0.5%v/v, and control cultures were treated with the same amount of solvent. Six days after plating, the cells were treated with or without 500 μM 1-aminobenzotriazole (ABT; Sigma-Aldrich), a broad inhibitor of CYPs, for 24 hr. After treatment, the cells were exposed to loratadine and desloratadine in the presence or absence of 500 μM ABT for 48 hr. The experiments were conducted in triplicate for each concentration.

Tomida T., Ishimura M, & Iwaki M. (2017). A cell-based assay using HepaRG cells for predicting drug-induced phospholipidosis. The Journal of toxicological sciences, 42(5).

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Evaluating 4aa for Trypanosoma Infection

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All procedures were performed in the Institute of Parasitology and Biomedicine Lopez-Neyra (Spanish National Research Council, CSIC) and approved according with the European and Spanish ethical regulations. Infection was carried out in female NMRI mice (Charles River Laboratories) by i.p. injection of 10⁴ bloodstream forms of T. brucei brucei (STIB795) in 0.2 mL TDB glucose from a cryopreserved stock. Three days later, infected animals with confirmed parasitemia were distributed into two groups: control (infected mice treated with vehicle (4 % DMSO, 20 % Captisol®), n=3) and treated (infected mice treated with 30 mg/kg/day of 4aa, n=3). An additional group was used for PK analysis (non-infected mice treated with 30 mg/kg/day of 4aa, n=3). All mice received a 0.2 mL i.p. injection at day 3 from infection during 5 consecutive days. Pre-treatment with 50 mg/kg/day ABT (1-Aminobenzotriazole, Sigma-Aldrich), a known inhibitor of CYP enzymes, 1 h prior compound injection was administered. Blood samples from tail tip were mixed with sterile water (ratio 1:2). To eliminate the risk of infections, the samples undergo 3 freeze-thaw cycles. Parasitemia was periodically examined, diluting 2 μL of blood from infected mice tail in 100 μL of TDB glucose and counting in a Neubauer chamber in a light microscope, and the day of death was recorded.

Singh B., Diaz-Gonzalez R., Ceballos-Perez G., Rojas-Barros D.I., Gunaganti N., Gillingwater K., Martinez-Martinez M.S., Manzano P., Navarro M, & Pollastri M.P. (2020). Medicinal chemistry optimization of a diaminopurine chemotype: Towards a lead for Trypanosoma brucei inhibitors. Journal of medicinal chemistry, 63(17), 9912-9927.

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Odorant Compound Preparation and CYP Inhibition

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The odorant compounds were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan) or Sigma-Aldrich Co. (St. Louis, MO). Odorant solutions were diluted to 1 M stock solutions in DMSO and kept at −20 °C until used. The CYP inhibitor ABT (1-aminobenzotriazole) was purchased from Sigma-Aldrich Co. and diluted to 400 µM in PBS.

Asakawa M., Fukutani Y., Savangsuksa A., Noguchi K., Matsunami H, & Yohda M. (2017). Modification of the response of olfactory receptors to acetophenone by CYP1a2. Scientific Reports, 7, 10167.

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