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Ki 67 fitc b56

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Ki-67 FITC (B56) is a fluorochrome-conjugated antibody that binds to the Ki-67 antigen, a nuclear protein expressed during active phases of the cell cycle. This product can be used in flow cytometry and immunohistochemistry applications to detect and quantify proliferating cells.

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Lab products found in correlation

Ifn α, by Merck Group (1 mentions) Cd19 pe lt19, by Miltenyi Biotec (1 mentions) Cd138 allophycocyanin b b4, by Miltenyi Biotec (1 mentions) Cd38 af700, by BioLegend (1 mentions) Cd20 efluor v450 2h7, by Thermo Fisher Scientific (1 mentions) Cd27 af647 lt27, by Bio-Rad (1 mentions) Cd27 fitc m t271, by BD (1 mentions) Cd19 percp cy5.5 sj225c1, by BD (1 mentions) Cd24 fitc ml5, by BD (1 mentions) Cd95 bv421, by BioLegend (1 mentions) Cd27 bv605, by BioLegend (1 mentions) Cd3viogreen bw264 56, by Miltenyi Biotec (1 mentions) Unconjugatedgoat anti irf4 m 17, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat igg af488, by Thermo Fisher Scientific (1 mentions) Goat anti human f ab 2 fragments anti igm and igg, by Jackson ImmunoResearch (1 mentions) Human il 2, by Thermo Fisher Scientific (1 mentions) Hybridomax hybridoma growth supplement, by Gentaur (1 mentions) Mem amino acids solution, by Merck Group (1 mentions) Il 21, by Thermo Fisher Scientific (1 mentions) Lipid mixture 1, by Merck Group (1 mentions)

5 protocols using ki 67 fitc b56

1

Multiparametric Flow Cytometry

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The following anti-human mAbs were used: CD3 APC-Cy7 (HIT3a), CD4 Pacific Blue (OKT4), CD38 APC (HIT2), CD45RA Brilliant Violet 421 (Hl100), CD127 Brilliant Violet 421 (A019D5), CD197 (CCR7) Alexa Fluor 647 (G043H7), HLA-DR PE-Cy7 (L243) and IL-2 FITC (MQ1-17H12) purchased from BioLegend; CD4 V500 (RPA-T4), CD8 V500 (RPA-T8), Bcl-2 FITC (Bcl-2/100) and Ki-67 FITC (B56) purchased from BD Biosciences; IFN-γ PE-Cy7 (4S.B3) obtained from eBioscience and CD40L PE (TRAP 1) obtained from Beckman Coulter.
Li Causi E., Parikh S.C., Chudley L., Layfield D.M., Ottensmeier C.H., Stevenson F.K, & Di Genova G. (2015). Vaccination Expands Antigen-Specific CD4+ Memory T Cells and Mobilizes Bystander Central Memory T Cells. PLoS ONE, 10(9), e0136717.
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2

Multiparametric Flow Cytometry of B Cells

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Antibodies used were CD19 PE (LT19; Miltenyi), CD138 allophycocyanin
(B-B4; Miltenyi Biotec), CD38 PE-Cy7 (HB7; BD Biosciences), CD38 AF700 (HIT2;
Biolegend), CD20 efluor V450 (2H7; eBioscience); CD27 AF647 (LT27; AbD Serotec),
CD27 FITC (M-T271), CD19 PerCP-Cy5.5 (SJ225C1; BD Biosciences), CD19 PE-Cy7
(SJ225C1; BD Biosciences), CD24 FITC(ML5; BD Biosciences), CD84 PE (CD84.1.21;
Biolegend), CD38 PerCP-Cy5.5 (HIT2; BD Biosciences), CD95 BV421 (DX2;
Biolegend), CD20 APC-H7(L27; BD Biosciences), CD27 BV605 (O323; Biolegend), CD3
VioGreen (BW264/56; Miltenyi), Ki67 FITC (B56; BD Biosciences), unconjugated
goat anti-IRF4 (M-17; Santa Cruz) and donkey anti-goat IgG AF488 (Polyclonal;
Invitrogen). Controls were isotype-matched mouse mAbs. Annexin V FITC was from
eBioscience and 7-AAD from BD Biosciences.
Reagents included human IL-2 (Roche); IL-6 (Peprotech) and IFN-α
(Sigma); IL-21 (PeproTech); goat anti-human F(ab′)2 fragments
(anti-IgM and -IgG; Jackson Immunoresearch); HybridoMax hybridoma growth
supplement (Gentaur); Lipid Mixture 1, chemically defined (200X) and MEM Amino
Acids Solution (50X; Sigma).
Arumugakani G., Stephenson S.J., Newton D.J., Rawstron A., Emery P., Doody G.M., McGonagle D, & Tooze R.M. (2017). Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. The Journal of Immunology Author Choice, 198(12), 4618-4628.
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3

Flow Cytometry Analysis of T Cell Subsets

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Whole blood was stained for flow cytometry with multiple combinations of the following mAbs: CD3-FITC (SP34) ; CD20-PE (3G8); CD8-PerCP (SK1); CD4-APC (L200); CD25-FITC (2A3); HLA-DR-PerCP (l243); Ki-67-FITC (B56) (all from BD Biosciences) and FoxP3-APC (PCH101) (EBiosciences), as described (17 (link), 18 (link)). Data were acquired with a FACSCalibur flow cytometer (BD Immunocytometry Systems) and analyzed with CellQuest software (BD Biosciences). CD4+ and CD8+ T cell percentages were obtained by first gating on lymphocytes and then on CD3+ T cells. Immune activation, and proliferation markers were determined by gating on lymphocytes, then on CD3+ T cells, and finally on CD4+CD3+ or CD8+CD3+ T cells.
He T., Brocca-Cofano E., Policicchio B.B., Sivanandham R., Gautam R., Raehtz K.D., Xu C., Pandrea I, & Apetrei C. (2016). T Regulatory Cell Depletion Reactivates Latent SIV in Controller Macaques while Boosting SIV-specific T Lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 197(12), 4535-4539.
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4

Multiparametric Flow Cytometry Staining

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For staining, 2 × 106 cells were stained with Zombie Aqua Viability Dye (BioLegend) in PBS for 10 min on ice, then Fc receptors were blocked with anti-CD16/CD32 antibodies from BD Biosciences (2.4G2) for an additional 10 min. After centrifugation, the supernatant was removed and cell were stained with a surface antibody cocktail containing in FACS buffer (PBS, 2 mM EDTA, 2% FBS) and Brilliant Stain Buffer Plus from BD Biosciences for 20 min on ice. The following antibodies were purchased from BioLegend; F4/80-PerCP/Cy5.5 (BM8), CD11c-PE/Cy7 (N418), CCR7-PE (4B12), CD90.2-A700 (30-H12), CD19-A700 (6D5), MHC-II-BV421 (M5/114.14.2), CD11b-BV605 (M1/70), CD8α-BV650 (53-6.7), Ly-6C-BV711 (HK1.4) and IL-12 PE (C15.6). CD40-FITC (HM40-3), CD103-APC (2E9), CD24-APC e780 (M1/69) and Granzyme B eFluor450 (NGZB) were obtained from Thermo Fisher Scientific. CD80-PE CF594 (16-10A1), CD45-BV786 (30-F11) and Ki-67 FITC (B56) were purchased from BD Biosciences. All samples were resuspended in FACS buffer and acquired on a BD Fortessa flow cytometer. Data were analyzed using FlowJo software from Tree Star, v10.5.
Blair T., Baird J., Bambina S., Kramer G., Gostissa M., Harvey C.J., Gough M.J, & Crittenden M.R. (2022). ICOS is upregulated on T cells following radiation and agonism combined with radiation results in enhanced tumor control. Scientific Reports, 12, 14954.
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5

Flow Cytometry Analysis of T Cell Subsets

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The following antibodies were used in this study: CD3ε-eFlour 450 (145-2C11, eBioscience), CD3ε-APC-eFluor 780 (17A2, eBioscience), CD8α-APC-eFluor 780 (53-6.7, eBioscience), CD8α-BV605 (53-6.7, Biolegend), CD8α-PerCP-Cy5.5 (53-6.7, eBioscience), CD44-PE-Cy7 (IM7, Biolegend), CD62L-APC (MEL-14, eBioscience), CD62L-BV510 (MEL-14, Biolegend), CD69eFluor 450 (H1.2F3, eBioscience) CD69-biotin (H1.2F3, eBioscience), CD122-FITC (TM-B1, BD Biosciences), CD127-BV605 (A7R34, Biolegend), and Streptavidin PerCP-Cy5.5 (BD Biosciences). The MHC class I tetramers H2-DbGP33–41 APC and H2-DbNP396–404 PE were kind gifts from Dr. Ramon Arens (LUMC, Leiden, The Netherlands). Cells were fixed with Foxp3/Transcription Factor Staining buffer set (eBioscience) and stained with Ki-67 PE or Ki-67 FITC (B56, BD Biosciences). Samples were acquired with the LSR Fortessa (BD) and analyzed with FlowJo software (Tree Star, Inc.).
Geerman S., Hickson S., Brasser G., Pascutti M.F, & Nolte M.A. (2016). Quantitative and Qualitative Analysis of Bone Marrow CD8+ T Cells from Different Bones Uncovers a Major Contribution of the Bone Marrow in the Vertebrae. Frontiers in Immunology, 6, 660.
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