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Anti pmp70

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Anti-PMP70 is a primary antibody that specifically targets the peroxisomal membrane protein 70 (PMP70). PMP70 is a member of the ATP-binding cassette (ABC) transporter family and plays a role in the transport of various molecules across the peroxisomal membrane. This antibody can be used for the detection and analysis of PMP70 in various experimental applications.

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Lab products found in correlation

Anti catalase, by Santa Cruz Biotechnology (2 mentions) Lab tek 2 chambered coverglass, by Thermo Fisher Scientific (2 mentions) Unmodified dna oligonucleotides, by Integrated DNA Technologies (2 mentions) Fluorescently modified dna oligonucleotides, by Bio-Synthesis (2 mentions) Biotinylated monoclonal antibodies against β tubulin and cox 4, by Cell Signaling Technology (2 mentions) Anti tgn46, by Avantor (2 mentions) Glass slides and coverslips, by Avantor (2 mentions) M13mp18 scaffold, by New England Biolabs (2 mentions) Formvar 400 mesh copper grids, by Electron Microscopy Sciences (2 mentions) Tetraspeck bead, by Thermo Fisher Scientific (2 mentions) Freeze n squeeze column, by Bio-Rad (2 mentions) Anti β actin, by Santa Cruz Biotechnology (2 mentions) Anti αsma, by Southern Biotech (1 mentions) Axiocam software, by Zeiss (1 mentions) Anti collagen 4, by Southern Biotech (1 mentions) Axiocam hrc digital camera, by Zeiss (1 mentions) Confocal microscope, by Zeiss (1 mentions) Anti nephrin, by Progen Biotechnik (1 mentions) Anti f4 80, by Santa Cruz Biotechnology (1 mentions) Tcs sp2 confocal microscope, by Leica (1 mentions)

Spelling variants of this product

PMP70 (6 citations) Rabbit anti-PMP70 (3 citations)

7 protocols using anti pmp70

1

Quantitative Kidney Histology Analysis

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For each mouse, quantitative analysis of glomerular volume, fractional mesangial area, and tuft area in paraffin-embedded kidney sections stained with PAS reagent was performed as described previously [33 (link)]. To examine collagen matrix, paraffin-embedded sections were stained with a picrosirius red stain [33 (link)]. Oil Red O staining was performed to evaluate lipid accumulation in frozen kidney tissues as described previously [33 (link)]. For immunohistochemistry, we used anti-nephrin (1:100; Progen biotechnik GmbH, Heidelberg, Germany), anti-F4/80 (1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-αSMA (1:200), and anti-collagen IV (1:200; Southern Biotechnology Associates, Birmingham, AL, USA) antibodies. Images were captured using a Zeiss microscope equipped with an Axio Cam HRC digital camera and Axio Cam software (Carl Zeiss, Thornwood, NY, USA). Staining intensities were then quantified using Image-Pro Plus 4.5 software (Media 149 Cybernetics, Silver Springs, MD, USA) as described previously [33 (link)]. Imaging for DAPI (1:1000), anti-PMP70 (1:200, Abcam, Cambridge, MA), and anti-catalase (1:200, Santa Cruz Biotechnology) antibodies was conducted using a confocal microscope (Carl Zeiss, Gottingen, Germany).
Kwon G., Uddin M.J., Lee G., Jiang S., Cho A., Lee J.H., Lee S.R., Bae Y.S., Moon S.H., Lee S.J., Cha D.R, & Ha H. (2017). A novel pan-Nox inhibitor, APX-115, protects kidney injury in streptozotocin-induced diabetic mice: possible role of peroxisomal and mitochondrial biogenesis. Oncotarget, 8(43), 74217-74232.
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2

Assessing Neuronal Morphology under Stress Conditions

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Primary rat cortical/hippocampal neurons were plated on coated 2 cm2 glass coverslips following the manufacturer's protocol (BrainBits®). All drug applications were performed blindly. After 7 days of differentiation, neurons were treated with the following concentrations: ADDL (1 μM), CAT-SKL (1 μM), Wy-14,643 (100 μM). Slides were washed three times with Hank's balanced salt solution between treatments, and then fixed and stained with the appropriate antibodies. Primary and secondary antibodies employed include: anti-MAP-2 (1:800) (Millipore) coupled with AlexaFluor 488 goat anti-mouse (1:500) (Life Technologies); and anti-PMP70 (1:500) (abcam®) coupled with AlexaFluor 546 goat anti-rabbit (1:15000) (Invitrogen). Microscopic analysis was performed using the Leica TCS SP2 confocal microscope located in the Department of Anatomy and Cell Biology's microscope facility at the Wayne State University School of Medicine, and Zeiss ApoTome Imaging System from MICR, also at the Wayne State University.
Giordano C.R., Terlecky L.J., Bollig-Fischer A., Walton P.A, & Terlecky S.R. (2014). Amyloid-beta neuroprotection mediated by a targeted antioxidant. Scientific Reports, 4, 4983.
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3

Immunoblotting Antibody Panel Validation

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The following antibodies were used for immunoblotting. Abcam: anti-PMP70 (1:1,000 dilution; ab3421), anti-FASN (1μg/ml dilution; ab22759); Cell Signaling: anti-cytochrome oxidase IV (1:250 dilution; 4850), anti-catalase (1:800 dilution; 12980), anti-centromere protein A (1:400 dilution; 2186), anti-calreticulin (1:200 dilution; 12238), anti-FASN (1:1,000 dilution; 3180); Santa Cruz Biotechnology: anti-C13orf31 (1:200 dilution; sc-374553; E7 and 1:500 dilution; sc-376231; E12), anti-caspase 1 p20 (1:250 dilution; sc-1218-R); R&D Systems: anti-IL-1β (1:250 dilution; AF-401-NA); Sigma: anti-β-actin (1:10,000 dilution; A5060). All antibodies used have validation profiles on either Antibodypedia or 1DegreeBio.
The following reagents were used: M-CSF (Peprotech, 300-25), LPS (from Escherichia coli K12, InvivoGen, tlrl-peklps), human IFN-γ (Peprotech, 300-02), murine IFN-γ (Peprotech, 315-05), human IL-4 (Peprotech, 200-04), murine IL-4 (Peprotech, 214-14), ATP (Sigma Aldrich, A2383), C75 (Sigma Aldrich, C5490), etomoxir (Sigma Aldrich, E1905), MitoTEMPO (Sigma Aldrich, SML0737), oligomycin (Sigma Aldrich, O4876), FCCP (Sigma Aldrich, C2920), rotenone (Sigma Aldrich, R8875), antimycin A (Sigma Aldrich, A8674), palmitate-BSA (Seahorse Bioscience, 102720-100), zymosan A (Sigma Aldrich, Z4250), PMA (Sigma Aldrich, P1585), HRP (Sigma Aldrich, P8375), luminol (Sigma Aldrich, A8511).
Cader M.Z., Boroviak K., Zhang Q., Assadi G., Kempster S.L., Sewell G.W., Saveljeva S., Ashcroft J.W., Clare S., Mukhopadhyay S., Brown K.P., Tschurtschenthaler M., Raine T., Doe B., Chilvers E.R., Griffin J.L., Kaneider N.C., Floto R.A., D’Amato M., Bradley A., Wakelam M.J., Dougan G, & Kaser A. (2016). C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nature immunology, 17(9), 1046-1056.
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4

DNA Origami Structures Visualization

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Unmodified DNA oligonucleotides were purchased from Integrated DNA Technologies. Fluorescently modified DNA oligonucleotides were purchased from Biosynthesis. Biotinylated monoclonal antibodies against β-tubulin and COX IV were purchased from Cell Signaling. Anti-PMP70 was purchased from Abcam. Anti-TGN46 was purchased from VWR. Streptavidin was purchased from Invitrogen (Catalog number: S-888). Bovine serum albumin (BSA), and BSA-biotin was obtained from Sigma Aldrich (Catalog Number: A8549). Glass slides and coverslips were purchased from VWR. Lab-Tek II chambered cover glass were purchased from Thermo Fisher Scientific. M13mp18 scaffold was obtained from New England Biolabs. p8064 scaffold for microtubule-like DNA origami structures was prepared as described before19 (link). ‘Freeze N Squeeze’ columns were ordered from Bio-Rad. TetraSpeck Beads were purchased from Life Technologies. Paraformaldehyde, glutaraldehyde and TEM grids (FORMVAR 400 Mesh Copper Grids) were obtained from Electron Microscopy Sciences.
Three buffers were used for sample preparation and imaging: Buffer A (10 mM Tris-HCl, 100 mM NaCl, 0.05 % Tween-20, pH 7.5), buffer B (5 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, 0.05 % Tween-20, pH 8), and buffer C (1×PBS, 500 mM NaCl, pH 8).
Jungmann R., Avendano M.S., Woehrstein J.B., Dai M., Shih W.M, & Yin P. (2014). Multiplexed 3D Cellular Super-Resolution Imaging with DNA-PAINT and Exchange-PAINT. Nature methods, 11(3), 313-318.
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5

Immunoblotting Analysis of PMP70 and FAS

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For peroxisome membrane protein 70 (PMP70) and fatty acid synthase (FAS) immunoblotting, 100 mg of BAT was homogenized in RIPA buffer (0.5% NP‐40, 0.1% sodium deoxycholate, 150 mmol L−1 NaCl, 50 mmol L−1 Tris‐Cl, pH 7.5). After centrifugation at 15 000× g for 10 minutes at 4°C, the supernatant was collected and kept at −80°C. Lysates were resolved by SDS‐PAGE, transferred to PVDF membrane (Milipore, Billerica, MA, USA), and probed with anti‐PMP70 (Abcam, Cambridge, UK), anti‐FAS (Cell Signaling Technology, Danvers, MA, USA) and anti‐β‐actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Chen C., Wang H., Chen B., Chen D., Lu C., Li H., Qian Y., Tan Y., Weng H, & Cai L. (2018). Pex11a deficiency causes dyslipidaemia and obesity in mice. Journal of Cellular and Molecular Medicine, 23(3), 2020-2031.
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6

DNA Origami Structures Visualization

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Unmodified DNA oligonucleotides were purchased from Integrated DNA Technologies. Fluorescently modified DNA oligonucleotides were purchased from Biosynthesis. Biotinylated monoclonal antibodies against β-tubulin and COX IV were purchased from Cell Signaling. Anti-PMP70 was purchased from Abcam. Anti-TGN46 was purchased from VWR. Streptavidin was purchased from Invitrogen (Catalog number: S-888). Bovine serum albumin (BSA), and BSA-biotin was obtained from Sigma Aldrich (Catalog Number: A8549). Glass slides and coverslips were purchased from VWR. Lab-Tek II chambered cover glass were purchased from Thermo Fisher Scientific. M13mp18 scaffold was obtained from New England Biolabs. p8064 scaffold for microtubule-like DNA origami structures was prepared as described before19 (link). ‘Freeze N Squeeze’ columns were ordered from Bio-Rad. TetraSpeck Beads were purchased from Life Technologies. Paraformaldehyde, glutaraldehyde and TEM grids (FORMVAR 400 Mesh Copper Grids) were obtained from Electron Microscopy Sciences.
Three buffers were used for sample preparation and imaging: Buffer A (10 mM Tris-HCl, 100 mM NaCl, 0.05 % Tween-20, pH 7.5), buffer B (5 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, 0.05 % Tween-20, pH 8), and buffer C (1×PBS, 500 mM NaCl, pH 8).
Jungmann R., Avendano M.S., Woehrstein J.B., Dai M., Shih W.M, & Yin P. (2014). Multiplexed 3D Cellular Super-Resolution Imaging with DNA-PAINT and Exchange-PAINT. Nature methods, 11(3), 313-318.
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7

Immunoblotting and Immunofluorescence Antibody Dilutions

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Antibodies used for immunoblotting and their corresponding dilutions were anti-MAVS (Santa Cruz Biotech sc-365334; 1:500), anti-TOM20 (Santa Cruz Biotech sc-11415; 1:300), anti-alpha Sr-1 (Abcam ab28052; 1:1,500), anti-β-actin (Santa Cruz Biotech sc-1615-hrp, 1:2,000), anti-MFN2 (Cell Signaling #9482; 1:800), anti-TBK1 (Cell Signaling #3504; 1:1,000), anti-phospho-TBK1 (Ser172) (Cell Signaling #5483; 1:700), goat anti-rabbit IgG-HRP (Millipore #12-348; 1:2000), and goat anti-mouse IgG-HRP (Millipore #12-349; 1:2,000). Primary antibodies used for immunofluorescence were anti-MAVS (Santa Cruz Biotech sc-365334; 1:100 dilution), anti-TOM20 (Abcam ab56783; 1:100 dilution), anti-FACL4 (Thermo PA5-27137; 1:50), anti-PMP70 (Abcam ab3424; 1:650), anti-MFN2 (Cell Signaling #9482; 1:50), anti-TRAF3 (Santa Cruz Biotech sc-1828or Thermo Fisher Scientific PA1-41107; 1:50), anti-alpha Sr-1 (Abcam ab28052; 1:50), anti-vimentin (Thermo Fisher Scientific PA1-16759; 1:2,000), anti-GM130 (BD Biosciences #610822; 1:250), anti-reovirus σ1 5C6 and G5 ((95 (link)); mix of 1:2,500 each; Fig. 6B), and rabbit anti-reovirus T3D antisera (B. Sherry, unpublished; 1:2,000; Fig S5). Secondary antibodies were Alexa® 488-, Alexa® 594- or Alexa® 647-conjugated goat anti-mouse, anti-rabbit or anti-chicken IgG (Thermo Fisher Scientific; 1:750 - 1:1,000).
Rivera-Serrano E.E., DeAngelis N, & Sherry B. (2017). Spontaneous Activation of a MAVS-dependent Antiviral Signaling Pathway Determines High Basal Interferon-β Expression in Cardiac Myocytes. Journal of molecular and cellular cardiology, 111, 102-113.
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