Unity inova 500 mhz

Manufactured by Agilent Technologies

Sourced in United States

The Unity Inova 500 MHz is a nuclear magnetic resonance (NMR) spectrometer that operates at a frequency of 500 MHz. It is designed to perform high-resolution NMR analysis of chemical samples.

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Lab products found in correlation

Lc 8a pump, by Shimadzu (3 mentions) P 2000 polarimeter, by Jasco (3 mentions) Cosmosil 5c18 ar 2 column, by Nacalai Tesque (3 mentions) J 715 spectropolarimeter, by Jasco (3 mentions) Sephadex lh 20, by GE Healthcare (3 mentions) Vnmrs 600 mhz, by Agilent Technologies (2 mentions) Ultimate 3000 uhplc system, by Thermo Fisher Scientific (2 mentions) Ft ir frontier instrument nicolet is10, by Thermo Fisher Scientific (2 mentions) Inova 600 mhz, by Bruker (2 mentions) Ascend 600 mhz, by Bruker (2 mentions) Spectrum 100 ftir spectrometer, by PerkinElmer (2 mentions) Synapt g2 qtof, by Waters Corporation (1 mentions) Avance 300 mhz, by Agilent Technologies (1 mentions) It tof hr mass spectrometer, by Shimadzu (1 mentions) Merck 60 f254, by Merck Group (1 mentions) Dimethyl sulfoxide d6, by Cambridge Isotopes (1 mentions) Benzene d6, by Cambridge Isotopes (1 mentions) Acetone d6, by Cambridge Isotopes (1 mentions) Chloroform d, by Cambridge Isotopes (1 mentions) Carbazole, by Merck Group (1 mentions)

Close spelling variants of this product

Inova 500 (72 citations) Unity Inova (51 citations) Unity Inova 500 MHz spectrometer (41 citations) Inova 500 MHz (30 citations) Unity Inova 500 (29 citations) Unity 500 (14 citations) Unity Inova 500 MHz NMR spectrometer (8 citations) Unity Inova 500 MHz NB High Resolution FT NMR (7 citations) Unity 500 MHz (5 citations) Unity Inova 500 MHz instrument (4 citations)

28 protocols using unity inova 500 mhz

Spectroscopy and Chromatography Methods

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General.
¹H and ¹³C NMR spectra were run on a Bruker Avance 300 MHz or a Varian Unity Inova 500 MHz NMR spectrometer. Mass spectra (MS) were run on a PerkinElmer Sciex API 150 EX mass spectrometer. High resolution mass spectra (HRMS) were run on a Waters Synapt G2 Q-TOF mass spectrometer in high-resolution mode. Column chromatography was carried out using a Teledyne Isco Combiflash Rf system with RediSep Rf silica cartridges. Preparative thin layer chromatography was carried out using Analtech TLC Uniplates (silica gel, 1000 mm, 20 cm × 20 cm). High pressure liquid chromatography was performed using a system consisting of a Waters 1525 pump unit, driven by Empower software, and a Waters 2487 detector. Microwave chemistry was carried out using a CEM Discover SP microwave with 10 mL irradiation tubes.

Seltzman H.H., Shiner C., Hirt E.E., Gilliam A.F., Thomas B.F., Maitra R., Snyder R., Black S.L., Patel P.R., Mulpuri Y, & Spigelman I. (2016). Peripherally Selective Cannabinoid 1 Receptor (CB1R) Agonists for the Treatment of Neuropathic Pain. Journal of medicinal chemistry, 59(16), 7525-7543.

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Spectroscopic Analysis of Organic Compounds

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Optical rotations were determined by using a P-2000 polarimeter (JASCO). Infrared (IR) spectra were recorded on a Mattson Genesis II spectrometer (Thermo). High-resolution electrospray ionization mass spectrometry (HRESIMS) data were obtained on an LCQ mass spectrometer (Thermo). Highresolution electrospray ionization mass spectrometry (HRESIMS) data were measured on a Shimadzu IT-TOF HR mass spectrometer. Nuclear magnetic resonance (NMR) spectra were recorded on Varian Unity Inova 500 MHz, or Varian VNMRS 600 MHz spectrometers. For column chromatography (CC), and silica gel 60 (70–230 and 230–400 mesh, Merck) were used. Precoated silica gel plates (Merck 60 F-254) were used for thin layer chromatography (TLC). High-performance liquid chromatography (HPLC) separations were performed on a Shimadzu LC-8A pump with a UV SPD-20A detector equipped with a 250 × 20 mm i.d. preparative Cosmosil 5C₁₈ AR-II column (Nacalai Tesque).

Huang H.T., Liaw C.C., Chiou C.T., Lee K.T, & Kuo Y.H. (2021). Mesonosides A-H, primeverose derivatives from Mesona procumbens suppress adipogenesis by downregulating PPARγ and C/EBPα in 3T3-L1 cells. Journal of Food and Drug Analysis, 29(3), 448-467.

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Proton NMR Characterization of Polymer Conversions

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Proton NMR was used to calculate all monomer conversions from vial
polymerizations. NMR spectra were obtained using a Varian Unity Inova
500 MHz spectrometer at room temperature with CDCl₃ as
the deuterated solvent. All spectra were recorded using 64 scans with
a relaxation delay time of 1 s. All chemical shifts were referenced
to chloroform.

Wilson O.R., Borrelli D.J, & Magenau A.J. (2022). Simple and Rapid Adhesion of Commodity Polymer Substrates under Ambient Conditions Using Complexed Alkylboranes. ACS Omega, 7(32), 28636-28645.

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Spectroscopic Characterization of Biomolecules

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UV/vis: Hitachi U3000 spectrophotometer,
λ_max [nm] (relative ε), in H₂O.
CD: Jasco J715 spectropolarimeter, λ_max and λ_min [nm] (Θ), in H₂O. ¹H nuclear
magnetic resonance (NMR): Bruker UltraShield 600 MHz or Varian Unity
Inova 500 MHz spectrometers, δ[ppm] with δ(HDO) = 4.79
ppm, in D₂O. Electrospray ionization mass spectrometry
(ESI-MS): Finnigan MAT 95S, m/z (rel.
intensity), positive-ion mode, 1.4 kV spray voltage; signals with
>5% rel. intensity are listed.

Roiser M.H., Müller T, & Kräutler B. (2015). Colorless Chlorophyll Catabolites in Senescent Florets of Broccoli (Brassica oleracea var. italica). Journal of Agricultural and Food Chemistry, 63(5), 1385-1392.

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NMR Characterization of Biomolecular Samples

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NMR samples were prepared at a concentration of about 1.5 mM, in 0.6 ml (H₂O/D₂O 9:1 v/v) buffer solution having 10 mM KH₂PO₄/K₂HPO4, 70 mM KCl and 0.2 mM EDTA (pH 7.0). All the samples were heated for 5–10 min at 90°C and slowly cooled (10–12 h) to room temperature. The solutions were equilibrated for several days at 4°C. The annealing process was assumed to be complete when ¹H-NMR spectra were superimposable on changing time. NMR spectra were recorded with Varian Unity INOVA 500 MHz spectrometer. 1D proton spectra of the sample in H₂O were recorded using pulsed-field gradient DPFGSE for H₂O suppression. ¹H-chemical shifts were referenced relative to external sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS). Pulsed-field gradient DPFGSE sequence was used for NOESY (180 ms and 80 ms mixing times) and TOCSY (120 ms mixing time) experiments in H₂O. All experiments were recorded using STATES-TPPI procedure for quadrature detection. In all 2D experiments, the time domain data consisted of 2048 complex points in t2 and 400–512 fids in t1 dimension. A relaxation delay of 1.2 s was used for all experiments.

Virgilio A., Esposito V., Pecoraro A., Russo A., Vellecco V., Pepe A., Bucci M., Russo G, & Galeone A. (2020). Structural properties and anticoagulant/cytotoxic activities of heterochiral enantiomeric thrombin binding aptamer (TBA) derivatives. Nucleic Acids Research, 48(22), 12556-12565.

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NMR Characterization of Organic Compounds

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All NMR experiments
were performed
at 25 °C on a Varian Unity Plus 300 spectrometer or Varian Unity-Inova
500 MHz spectrometer equipped with a 5 mm triple-resonance ¹H(¹³C/¹⁵N), z-axis pulsed-field
gradient probe head. For characterization purposes, samples consisted
of a ∼5 mM solution of each compound in chloroform-d (99.8% D, Cambridge Isotopes), dimethyl sulfoxide-d₆ (99.9% D, Cambridge Isotopes), benzene-d₆ (99.5%
D, Cambridge Isotopes) or acetone-d₆ (99.9%
D, Cambridge Isotopes), and the spectra were referenced to residual
solvent peaks at 7.27, 2.50, 7.16, and 2.05 ppm, respectively. ¹H-1D spectra were acquired at a resolution of 16k complex
points in the time domain with 32 accumulations each (sw = 6000 Hz,
d1 = 3 s).

Mahajan S.S., Scian M., Sripathy S., Posakony J., Lao U., Loe T.K., Leko V., Thalhofer A., Schuler A.D., Bedalov A, & Simon J.A. (2014). Development of Pyrazolone and Isoxazol-5-one Cambinol Analogues as Sirtuin Inhibitors. Journal of Medicinal Chemistry, 57(8), 3283-3294.

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Synthesis and Characterization of Pyrogallol[4]arene Compounds

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Reagents, solvents, guest molecules, and synthetic precursors were purchased from commercial suppliers at ACS Reagent Grade or equivalent purity and used without further purification. Pyrogallol, butrylaldehyde, anthracene, fluoranthene, pyrene, and fluorene were obtained from Acros Organics. Hydrochloric acid, ethanol, and methanol were obtained from Fisher Scientific. Undecanal and 1,5-diaminonaphthalene were obtained from TCI. Carbazole and coumarin were obtained from Sigma-Aldrich. 1-Adamantanecarboxylic acid and [2.2]paracyclophane were obtained from Alfa Aesar and Combi-blocks respectively. Deuterated solvents for NMR spectroscopy were purchased from Cambridge Isotopes. CDCl₃ was filtered through basic alumina prior to use. Pyrogallol[4]arenes 1a and 1b were synthesized using published procedures.26 ,32
¹H and ¹³C solution NMR spectra were acquired using a Bruker Avance IIIHD 600 MHz, a Varian Unity INOVA 500 MHz, or a Varian Mercury 400 MHz spectrometer. Residual solvent peaks were used as internal standards: CHCl₃ (δ^H = 7.26 ppm; δ^C = 77.16 ppm), benzene (δ^H = 7.16 ppm; δ^C = 128.06 ppm). NMR measurements performed in CCl₄ used a coaxial NMR tube insert and C₆D₆ for an external solvent lock and chemical shift referencing.

Journey S.N., Teppang K.L., Garcia C.A., Brim S.A., Onofrei D., Addison J.B., Holland G.P, & Purse B.W. (2017). Mechanically induced pyrogallol[4]arene hexamer assembly in the solid state extends the scope of molecular encapsulation †Electronic supplementary information (ESI) available: Experimental procedures, NMR spectra, and tabulated data. See DOI: 10.1039/c7sc03821f. Chemical Science, 8(11), 7737-7745.

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Spectroscopic and Chromatographic Analysis

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Optical rotations were measured on a Rudolph Research Autopol III automatic polarimeter. ECD measurements were performed using a JASCO J-715 spectropolarimeter. 1D and 2D NMR data were recorded on Varian Unity Inova 500 MHz FT-NMR instruments, and the data were processed using MestReNova 10.0 software (Mestrelab Research SL, Santiago de Compostela, Spain). Mass spectrometry experiments were performed on an Agilent 6538 HRESI QTOF MS system coupled to an Agilent 1290 HPLC system. HP-20ss (Sorbent Technologies, Norcross, GA, USA) and Sephadex LH-20 (GE Healthcare Bio-Science AB, Uppsala, Sweden) were used for open-column chromatography. Thin-layer chromatographic (TLC) analyses were conducted using Kieselgel 60 F254 plates (silica gel, 0.25 mm layer thickness, Merck, Germany) with visualization under UV light (254 and 365 nm). HPLC separations were performed on a Shimadzu system using a SCL-10A VP pump and system controller with a Phenomenex Gemini 5 μm C₁₈ column (110 Å, 250 × 21.2 mm, 10 mL/min) for preparative HPLC, and on a Waters system using a Waters 1525 binary pump and Waters 2998 photodiode array detector with a Phenomenex Gemini 5 μm C₁₈ column (110 Å, 250 × 10.0 mm, 4 mL/min) for semi-preparative HPLC. All solvents were of ACS grade or better.

Kil Y.S., Risinger A.L., Petersen C.L., Liang H., Grkovic T., O’Keefe B.R., Mooberry S.L, & Cichewicz R.H. (2020). Using the Cancer Dependency Map to Identify the Mechanism of Action of a Cytotoxic Alkenyl Derivative from the Fruit of Choerospondias axillaris. Journal of natural products, 83(3), 584-592.

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NMR Characterization of Cyclodextrin Complexes

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Samples of equivalent concentrations (9 mM) of BCL, SBE-β-CyD, HP-β-CD, and the corresponding inclusion complexes were prepared in a D₂O/CD₃OD (8:2, v/v) solution and transferred to 5-mm NMR tubes for spectrum acquisition. All spectra were recorded at 300 K with a Varian Unity Inova 500 MHz (11.75 T) instrument. The deuterated methanol (3.30 ppm) was used as an internal reference, to avoid the addition of external references that could interact with the CyDs.

De Gaetano F., Cristiano M.C., Paolino D., Celesti C., Iannazzo D., Pistarà V., Iraci N, & Ventura C.A. (2022). Bicalutamide Anticancer Activity Enhancement by Formulation of Soluble Inclusion Complexes with Cyclodextrins. Biomolecules, 12(11), 1716.

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NMR Spectroscopy of Micro RNA Samples

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¹H-NMR spectra were acquired at 5, 25, 37 and 49 °C either on a Varian Unity Inova 700 MHz spectrometer equipped with an HCN triple resonance cryoprobe or on a Varian Unity INOVA 500 MHz spectrometer equipped with a broadband inverse probe with z-field gradient and processed using the Varian VNMR and iNMR (http://www.inmr.net) software packages. All micro RNA samples were prepared at ~0.3 mM concentration by dissolving 75 nmol of each miRNA in 250 µL of 10 mM PBS buffer at pH 6.8. The spectra were acquired as 16,384 data points with a recycle delay of 1.0 s; data sets were zero-filled to 32,768 points prior to Fourier transformation and apodized with a shifted sine bell squared window function. Water suppression was achieved by including a double pulsed-field gradient spin-echo (DPFGSE) module^{45 (link),46 (link)} in the pulse sequence prior to acquisition.

Gangemi C.M., Alaimo S., Pulvirenti A., García-Viñuales S., Milardi D., Falanga A.P., Fragalà M.E., Oliviero G., Piccialli G., Borbone N., Ferro A., D’Urso A., Croce C.M, & Purrello R. (2020). Endogenous and artificial miRNAs explore a rich variety of conformations: a potential relationship between secondary structure and biological functionality. Scientific Reports, 10, 453.

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