Acrylamide bis acrylamide 19 1 40 solution electrophoresis

Reagents were purchased from the following suppliers and were of the highest purity available. Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA) or, in the case of DHT-containing Oligonucleotides, from Midland Certified Reagent Co. (Midland, TX). Uracil DNA glycosylase (UDG), and T4 DNA polynucleotide kinase (T4 PNK), formamidopyrimidine DNA glycosylase (Fpg) and Endonuclease III (Nth) were from New England Biolabs (Ipswich, MA). [γ-³²P]-ATP (6000 Ci/mmol) was purchased from PerkinElmer. C-18 Sep-Pak cartridges were purchased from Waters (Milford, MA), and BS Poly-prep columns were obtained from BioRad (Hercules, CA). Acrylamide/bis-acrylamide 19:1 (40% Solution/Electrophoresis) was purchased from Fisher Scientific (Waltham, MA), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO). Quantification of radioactivity in polyacrylamide gels was carried out using a Personal Molecular Imager (BIORAD) with Quantity One software (v.4.6.5).

Oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA). Uracil DNA glycosylase (UDG), ϕ29 DNA polymerase, and T7 DNA polymerase were from New England Biolabs (Ipswich, MA). A mixture of the four 2′-deoxynucleotide triphosphates (dNTP) was purchased from Promega (Madison, WI). [γ-³²P]-ATP (6000 Ci/mmol) was purchased from Perkin-Elmer. C-18 Sep-Pak cartridges were purchased from Waters (Milford, MA), and BS Poly-prep columns were obtained from BioRad (Hercules, CA). Acrylamide/bis-acrylamide 19:1 (40% Solution/Electrophoresis) was purchased from Fisher Scientific (Waltham, MA), and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO).