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Ic002f

Manufactured by R&D Systems
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The IC002F is a laboratory instrument designed for immunocytochemical (ICC) and immunohistochemical (IHC) staining procedures. It is a compact, automated system that performs slide-based staining of fixed cells or tissue sections. The IC002F can handle multiple sample slides simultaneously and automates the critical steps of the staining process, including reagent addition, incubation, and washing. This equipment is intended for use in research and clinical laboratory settings to facilitate standardized and reproducible immunostaining workflows.

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Lab products found in correlation

Ic002p, by R&D Systems (3 mentions) Ab33858, by Abcam (1 mentions) Sc 7324, by Santa Cruz Biotechnology (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) E cadherin, by R&D Systems (1 mentions) Anti ccl8 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) 40 μm pore cell strainer, by BD (1 mentions) Anti fibronectin, by R&D Systems (1 mentions) Rtgf β, by R&D Systems (1 mentions) Facsdiva, by BD (1 mentions) Facscalibur system, by BD (1 mentions) Anti cd90, by BD (1 mentions) Fab170p, by R&D Systems (1 mentions) Ab1791, by Abcam (1 mentions) Gtx61796, by GeneTex (1 mentions) Gtx124222, by GeneTex (1 mentions) Fab357p, by R&D Systems (1 mentions) Fab200f, by R&D Systems (1 mentions)

6 protocols using ic002f

1

Flow Cytometry Characterization of Dental Stem Cells

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For the flow cytometry assay, both hTDSCs and dTDSCs at P3 were used. 1 × 106 cells of each group were incubated with 1 mg of phycoerythrin-conjugated or fluorescein-isothiocyanate-conjugated monoclonal antibodies (R&D Systems) at 4 °C for 1 h. Anti-CD34 (sc-7324; Santa Cruz Biotechnology, Santa Cruz, CA), anti-CD31 (ab33858; Abcam, Cambridge, UK), anti-CD44 (BD550974; BD Bioscience), anti-CD45 (BD559135; BD Bioscience), and anti-CD90 (BD554898; BD Bioscience) were the antibodies used in this study. Phycoerythrin-conjugated or fluorescein-isothiocyanate-conjugated isotype-matched IgG1 were used as negative controls (IC002P or IC002F; R&D Systems). The stained cells were washed with ice-cold PBS containing 2% BSA before analysis using the LSRFortessa flow cytometer (Becton Dickinson, San Jose, CA). About 1 × 104 events were counted for each sample. The percentage of cells with a positive signal was calculated using the WinMDI Version 2.9 program (The Scripps Research Institute, La Jolla, CA).
SHI L., LI Y.J., DAI G.C., LIN Y.C., LI G., WANG C., CHEN H, & RUI Y.F. (2019). Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder. Stem Cell Research & Therapy, 10, 27.
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2

Fibrosis Induction and Quantification in Human Tubular Epithelial Cells

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A single-cell suspension was prepared by filtering the homogenate using a 40-μm pore cell strainer (BD Pharmingen, San Jose, CA, USA). Cells were incubated with Fc Block anti-CD16/32 (IC1918F, BD Pharmingen) and stained with fluorescein isothiocyanate-conjugated anti-fibronectin or isotype control (IC002F, R&D Systems) for 1 h. Fibronectin-positive cells and E-cadherin-positive cells were analyzed using a BD FACS Diva instrument (version 8.0; BD Biosciences). Fibrosis was induced in hTECs with 2 ng/mL rTGF-β (R&D Systems) for 48 h. To evaluate the role of CCL8 in fibrosis, rTGF-β-stimulated hTECs were simultaneously treated with an anti-CCL8 monoclonal antibody (50 or 100 ng/mL; Invitrogen). To quantify cell fibrosis and adhesion, cells were harvested and stained with antibodies against fibronectin (Invitrogen) and E-cadherin (R&D Systems) according to the manufacturers’ protocols. Apoptosis and necrosis were quantified by flow cytometry using an annexin V/propidium iodide assay. hTECs stained with propidium iodide and FITC-conjugated annexin V were incubated for 30 min in the dark, followed by analysis with the BD FACS Diva instrument.
Lee J., Lee Y., Kim K.H., Kim D.K., Joo K.W., Shin S.J., Kim Y.S, & Yang S.H. (2022). Chemokine (C-C Motif) Ligand 8 and Tubulo-Interstitial Injury in Chronic Kidney Disease. Cells, 11(4), 658.
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3

Flow Cytometric Analysis of CD44 and CD90

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Phycoerythrin (PE) or fluorescein isothiocyanate (FITC) (BD Biosciences)-conjugated mouse anti-rat monoclonal anti-CD44 (550974; BD Biosciences) and anti-CD90 (551401; BD Biosciences) were used. PE- or FITC-conjugated isotype-matched IgG1 were used as negative controls (IC002P or IC002F, R&D systems, Inc., Minneapolis, US). TDCs (5 × 105; P1) were incubated with 1 μg of antibodies away from the light for 45 min at room temperature. After washing and centrifugation at 4000× g for 5 min, the cells were resuspended in 300 μL of ice cold PBS (with 10% FBS and 1% sodium azide) and subjected to fluorescence-activated cell sorting (FACS) analysis (BD Biosciences). A BD FACSCalibur™ system (BD Biosciences) was used to calculate the percentage of cells with positive signals.
Hsiao M.Y., Lin P.C., Liao W.H., Chen W.S., Hsu C.H., He C.K., Wu Y.W., Gefen A., Iafisco M., Liu L, & Lin F.H. (2019). The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor. International Journal of Molecular Sciences, 20(14), 3437.
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4

Antibody Utilization for Western Blot, IF, and ChIP

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The following commercially available antibodies were used at the indicated concentrations for Western blot (WB) immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) analyses: JMJD3 (GTX124222, GeneTex, 1:250 IF, 1:500 WB); H3K27me3 (GTX121184, GeneTex, 1:1000 WB, 1–2 μg/ChIP); histone H3 (AB1791, Abcam, 1–2 μg/ChIP); β-actin (A2228, Sigma, 1:2000 WB); lamin A/C (GTX101126, GeneTex, 1:400 IF); γH2AX (GTX61796, GeneTex, 1:400 IF); and Alexa Fluor 488 Phalloidin (A12379, Molecular Probes, 1:40).
The following commercially available antibodies were used at the indicated dilutions for flow cytometry (FC) analyses: VEGFR-PE (FAB357P, R&D Systems, 1:50); IL-6Rα-PE (352803, BioLegend, 1:50); CXCR4-PE (FAB170P, R&D Systems, 1:50); uPAR-FITC (3936CJ, American Diagnostics, 1:50); CXCR1-Alexa647 (335201, BioLegend, 1:50); CXCR2-PE (32075, BioLegend, 1:50); CCR1-PE (335201, BioLegend, 1:50); CCR3-PE (310705, BioLegend, 1:50); CCR5-FITC (313705, BioLegend, 1:50); GM-CSFa-FITC (306906, BioLegend, 1:50); IgG-PE (IC002P, R&D Systems, 1:50); and IgG-fluorescein (IC002F, R&D Systems, 1:50).
Perrigue P.M., Silva M.E., Warden C.D., Feng N.L., Reid M.A., Mota D.J., Joseph L.P., Tian Y.I., Glackin C.A., Gutova M., Najbauer J., Aboody K.S, & Barish M.E. (2015). The Histone Demethylase Jumonji Coordinates Cellular Senescence Including Secretion of Neural Stem Cell-attracting Cytokines. Molecular cancer research : MCR, 13(4), 636-650.
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5

Immunofluorescent Imaging of IGF-IR in Glioma Cells

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Glioma cells were plated at a density of 4 × 104 cells per coverslip. After incubation for 24 h, cells were stained with mouse monoclonal anti-human IGF-IR fluorescein-conjugated antibody, clone #33255 (R&D Systems, #FAB391F, dilution 1:200) or mouse IgG1 fluorescein-conjugated isotype control antibody (R&D Systems, #IC002F, dilution 1:200). After staining, coverslips were mounted on slides with Fluoromount-G and imaged by fluorescence microscopy. Each experiment was performed in triplicate and assessed in a blinded manner.
Oliva C.R., Halloran B., Hjelmeland A.B., Vazquez A., Bailey S.M., Sarkaria J.N, & Griguer C.E. (2018). IGFBP6 controls the expansion of chemoresistant glioblastoma through paracrine IGF2/IGF-1R signaling. Cell Communication and Signaling : CCS, 16, 61.
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6

IL-1α Expression Profiling by Flow Cytometry

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Cells were detached (Accutase), fixed (2% formaldehyde; 5 min, RT), washed (BSA, 0.5%; NaN3, 0.05%; in PBS), Fc blocked (10 min, RT; BioLegend; 1:100), before incubation (30 min, RT) with anti‐IL‐1α‐FITC (FAB200F, R&D; 1:20) or isotype control‐FITC (IC002F, R&D; 1:20), before washing and analysis by flow cytometry (Accuri C6).
Wiggins K.A., Parry A.J., Cassidy L.D., Humphry M., Webster S.J., Goodall J.C., Narita M, & Clarke M.C. (2019). IL‐1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence‐associated secretory phenotype. Aging Cell, 18(3), e12946.
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