Vybrant cell labelling solutions

For experiments performed at MSKCC, all culture media including M199, α-MEM, Trypsin (0.25%)-EDTA (0.02%) and PBS, as well as Penicillin (10,000 U/mL)-Streptomycin (10,000 μg/mL) concentrate solution were prepared and supplied by the Memorial Sloan-Kettering Cancer Centre Culture Media Core Facility (New York, NY, USA). Amphotericin B was purchased from Life Technologies (Grand Island, NY, USA). Gelatin was from TJ Baker Inc. (Philipsburgh, NJ, USA). Bovine serum albumin was from Gemini Bioproducts (West Sacramento, CA, USA). Falcon tissue culture flasks and centrifuge tubes were purchased from BD Biosciences (Two Oak Park, Bedford, MA). Nunc plastic culture well coverslips were from ThermoFisher, Waltham, MA, USA). The lipophilic fluorescent probes DiD (excitation 644 nm, emission 665 nm) and DiO (excitation 484 nm, emission 501 nm) Vybrant cell labelling solutions were from Molecular Probes, Life Technologies (Grand Island, NY, USA), and were used in all experiments at both the MSKCC and CMPRU.

All cell culture media, phosphate buffered saline (PBS), trypsin (0.25%)/EDTA (1 mM) and bovine calf serum (BCS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Gelatin was from Sigma-Aldrich (St. Louis, MO, USA). Tissue culture plasticware was purchased from Costar (Cambridge, MA, USA). CSL Biosciences (Parkville, VIC, Australia) supplied antibiotics penicillin and streptomycin. ICN Biomedicals Inc. (Costa Mesa, CA, USA) provided amphotericin B. SAOS-2 osteosarcoma cells (HTB-85) and HDFs (HDF-616) were from the American Type Culture Collection (Manassas, VA, USA). The lipophilic fluorescent probes DiD (excitation 644 nm, emission 665 nm) and DiO (excitation 484 nm, emission 501 nm) Vybrant cell labelling solutions were from Molecular Probes, Life Technologies (Grand Island, NY, USA). The black-bottomed 24-well plates were from Ibidi (Gräfelfing, Barvaria, Germany).

All culture media including M199, α-MEM, M5, Trypsin (0.25%)-EDTA (0.02%) and PBS, as well as Penicillin (10,000 U/ml)-Streptomycin (10,000 μg/ml) concentrate solution were prepared and supplied by the Memorial Sloan-Kettering Cancer Centre Culture Media Core Facility (New York, NY). Amphoteracin B was purchased from Life Technologies (Grand Island, NY). Gelatin was from TJ Baker Inc (Philipsburgh, NJ). Bovine serum albumin was from Gemini Bioproducts (West Sacramento, CA). Falcon tissue culture flasks and centrifuge tubes were purchased from BDBiosciences (Two Oak Park, Bedford, MA). HDF were from The Coriell Institute (Camden, NJ). SAOS-2 osteosarcoma cells were from the American Type Culture Collection (VA, USA). A673 osteosarcoma and H3122 lung carcinoma cells were from the collection at the Memorial Sloan Kettering Cancer Center. The lipophilic fluorescent probes DiD (excitation 644nm, emission 665nm) and DiO (excitation 484nm, emission 501nm) Vybrant cell labelling solutions were from Molecular Probes, Life Technologies (Grand Island, NY). DAPI was provided by the FACS core facility at Memorial Sloan Kettering Cancer Center.