Anti dnm1l

Western blotting was carried out as described before.^{22 (link)} Total protein was extracted by RIPA buffer containing protease cocktail inhibitor. All protein samples were quantified. Protein samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes. After blocking with 5% skim milk in TBS-T (BD Pharmingen), PVDF membranes were incubated with the primary antibody overnight at 4 °C and then with the secondary antibody for 1 h at room temperature. The following antibodies were used: anti-MFN1 (1:1000, Abcam), anti-MFN2 (1:1000, Abcam), anti-Opa1 (1:1000, Abcam), anti-DNM1L (1:1000, Abcam), anti-MFF (1:1000, Abcam), anti-TOM20 (1:1000, Abcam), anti-E-cadherin (1:1000, Abcam), anti-N-cadherin (1:1000, Abcam), anti-Snail (1:1000, Abcam), anti-Twist (1:1000, Abclonal), anti-Zeb1 (1:1000, Abclonal), anti-Slug (1:1000, Abclonal) anti-Vimentin (1:1000, Abcam) and anti-β-actin (1:1000, Abcam). Protein bands were detected by using image acquisition using ImageQuant™ LAS 4000 (GE Healthcare Life Sciences).