Ym 58483 btp2

To block voltage-gated sodium channels, 1 μM Tetrodotoxin (Cat#1078; Tocris) was applied to the media before electrostimulation of spheroids. Control cells were treated with DMSO as vehicle. The following drugs and final concentrations were applied for pharmacological blockade on iMGLs exposed to spheroid conditioned media: 100 μM AR-C 66096 Tetrasodium Salt (Cat#3321; Tocris), 200 μM (+)-MK 801 Maleate (Cat#0924; Tocris), 200 μM PPADS Tetrasodium Salt (Cat#0625; Tocris), 200 μM MPEP Hydrochloride (Cat#1212; Tocris), 50 μM L-AP4 (Cat#0103; Tocris), 10 μM YM 58483 (BTP2) (Cat#3939; Tocris), 10 μM CNQX Disodium Salt (Cat#1045; Tocris), 50 μM D-AP5 (APV) (Cat#0106; Tocris), 100 μM PTX (Picrotoxin) (Cat#1128; Tocris). To induce lipid accumulation, 20 μM Oleic Acid (Cat# 03008; Sigma-Aldrich) was applied overnight to iMGLs, control cells were treated with 0.1% BSA as vehicle. 1 μM Triacsin C (Cat#10007448; Cayman Chemical) was applied to iMGLs overnight to deplete lipid accumulation.