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Sw28 centrifuge rotor

Manufactured by Beckman Coulter
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The SW28 centrifuge rotor is designed for high-speed centrifugation of samples in Beckman Coulter ultracentrifuges. It is capable of reaching a maximum speed of 28,000 rpm and can generate a maximum relative centrifugal force (RCF) of 141,000 x g. The rotor accommodates sample tubes with a volume capacity of up to 38 mL.

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Lab products found in correlation

Pspax2 packaging plasmid, by Addgene (2 mentions) Lentiviral mission short hairpin rna shrna clones targeting, by Merck Group (2 mentions)

Close spelling variants of this product

SW28 rotor (260 citations)

2 protocols using sw28 centrifuge rotor

1

Lentiviral shRNA Knockdown of UBE4B

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three lentiviral Mission short hairpin RNA (shRNA) clones targeting UBE4B were obtained from Millipore-Sigma. HEK293T cells were transfected with the lentiviral shRNA-targeting vectors, psPAX2 packaging plasmid (Addgene) and vesicular stomatitis virus glycoprotein (VSV-G). After 48 h, the supernatants were collected and centrifuged at 25,000 rpm using a Beckman SW28 centrifuge rotor for 2 h at 4°C. The supernatants were removed, and the pellets were re-suspended in ice-cold phosphate-buffered saline (PBS). Viral stocks were used to infect cell lines.
Mohanty S., Han T., Choi Y.B., Lavorgna A., Zhang J, & Harhaj E.W. (2020). The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation. PLoS Pathogens, 16(12), e1008504.
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2

Lentiviral shRNA Knockdown of UBE4B

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three lentiviral Mission short hairpin RNA (shRNA) clones targeting UBE4B were obtained from Millipore-Sigma. HEK293T cells were transfected with the lentiviral shRNA-targeting vectors, psPAX2 packaging plasmid (Addgene) and vesicular stomatitis virus glycoprotein (VSV-G). After 48 h, the supernatants were collected and centrifuged at 25,000 rpm using a Beckman SW28 centrifuge rotor for 2 h at 4°C. The supernatants were removed, and the pellets were re-suspended in ice-cold phosphate-buffered saline (PBS). Viral stocks were used to infect cell lines.
Mohanty S., Han T., Choi Y.B., Lavorgna A., Zhang J., & Harhaj E.W. (2020). The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation.
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