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Anti 1 a 1 e percp clone m5 114

Manufactured by BioLegend
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Anti-I-A/I-E-PerCP (clone M5/114.15.2) is a lab equipment product that recognizes the I-A/I-E major histocompatibility complex class II (MHC-II) antigen. It is conjugated to the PerCP (Peridinin Chlorophyll Protein Complex) fluorochrome.

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Lab products found in correlation

Anti cd86 pe clone gl 1, by BioLegend (3 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (3 mentions) Macsquant analyzer, by Miltenyi Biotec (3 mentions) Fixation buffer, by BioLegend (2 mentions) Intracellular staining perm wash buffer, by BioLegend (2 mentions) Anti ifn γ pe cyanine7 clone xmg1, by BioLegend (2 mentions) Anti il 4 pe clone 11b11, by BioLegend (2 mentions) Anti cd4 fitc clone gk1, by BioLegend (2 mentions)

3 protocols using anti 1 a 1 e percp clone m5 114

1

Monitoring T Cell Proliferation with CFSE

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CFSE (eBioscience Inc., San Diego, CA, USA) was used to monitor the proliferation of T cells. Surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1, BioLegend), respectively. Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotech) and analyzed with FlowJo (Tomy Digital Biology Co., Ltd., Tokyo, Japan).
Ando M., Nagata K., Takeshita R., Ito N., Noguchi S., Minamikawa N., Kodama N., Yamamoto A., Yashiro T., Hachisu M., Ichihara G., Kishino S., Yamamoto M., Ogawa J, & Nishiyama C. (2024). The gut lactic acid bacteria metabolite, 10-oxo-cis-6,trans-11-octadecadienoic acid, suppresses inflammatory bowel disease in mice by modulating the NRF2 pathway and GPCR-signaling. Frontiers in Immunology, 15, 1374425.
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2

Quantifying T Cell Proliferation and Cytokine Profiles

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To analyze the proliferation of T cells, CFSE (eBioscience Inc., San Diego, CA, USA) was used. Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1, BioLegend), respectively. Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11, BioLegend), respectively, with anti-CD4-FITC (clone GK1.5, BioLegend) after treatment with the Fixation Buffer (#420801, BioLegend) and the Intracellular Staining Perm Wash Buffer (#421002, BioLegend). Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotech) and analyzed with FlowJo (Tomy Digital Biology, Tokyo, Japan).
Kodama N., Okada H., Hachisu M., Ando M., Ito N., Nagata K., Katagiri M., Yasuda Y., Hiroki I., Yashiro T., Ichihara G., Yamamoto M, & Nishiyama C. (2023). A rose flavor compound activating the NRF2 pathway in dendritic cells ameliorates contact hypersensitivity in mice. Frontiers in Nutrition, 10, 1081263.
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3

Multiparametric Flow Cytometry Analysis

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To analyze the proliferation of T cells, CFSE (eBioscience Inc., San Diego, CA, USA) was used. Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1, BioLegend), respectively. Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11, BioLegend), respectively, with anti-CD4-FITC (clone GK1.5, BioLegend) after treatment with the Fixation Buffer (#420801, BioLegend) and the Intracellular Staining Perm Wash Buffer (#421002, BioLegend). Fluorescence was detected by a MACS Quant Analyzer (Miltenyi Biotech) and analyzed with FlowJo (Tomy Digital Biology, Tokyo, Japan).
Kodama N., Okada H., Hachisu M., Ando M., Ito N., Nagata K., Yasuda Y., Hiroki I., Yashiro T., Ichihara G., Yamamoto M., & Nishiyama C. (2022). A rose flavor compound activating the NRF2 pathway in dendritic cells ameliorates contact hypersensitivity in mice.
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