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Mouse cxcl1 kc elisa kit

Manufactured by R&D Systems
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Sourced in United States

The Mouse CXCL1/KC ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure mouse CXCL1/KC levels in cell culture supernatants, cell lysates, serum, and plasma.

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Lab products found in correlation

Ldh detection kit, by Roche (1 mentions) Fluorometric assay kit, by Merck Group (1 mentions) Pbs 14200 075, by Thermo Fisher Scientific (1 mentions) Human cxcl1 gro alpha elisa kit, by R&D Systems (1 mentions) Infinite m1000 pro, by Tecan (1 mentions)

Close spelling variants of this product

Mouse CXCL1/KC DuoSet ELISA kit Mouse CXCL1/KC Quantikine ELISA Kit CXCL1/KC ELISA Kit Mouse KC ELISA kit Mouse CXCL1 ELISA kit CXCL1/KC KC ELISA kit CXCL1 ELISA kit Mouse ELISA kits CXCL1

5 protocols using mouse cxcl1 kc elisa kit

1

Quantification of ATP and Cytotoxicity

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ATP in BALF was assessed using a Fluorometric Assay Kit (SIGMA ALDRICH, MO, USA). The LDH detection kit (Roche Diagnostics, Indianapolis, IN, USA) was used to assess the cytotoxic effect of different treatments. Data were expressed as the fold changes of different groups versus the control groups. Mouse CXCL1/KC ELISA kit (R&D Systems Inc. Minneapolis, MN, USA) was used to quantify KC in BALF.
Nouri H.R., Schaunaman N., Kraft M., Li L., Numata M, & Chu H.W. (2023). Tollip deficiency exaggerates airway type 2 inflammation in mice exposed to allergen and influenza A virus: role of the ATP/IL-33 signaling axis. Frontiers in Immunology, 14, 1304758.
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2

Murine Immune Response Assay

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Mouse IL-6 (431401), IL-10 (431411) and TNFα (430901) ELISA kits and Anti-F4/80 mouse BV421 antibody (123131) were purchased from BioLegend (San Diego, CA). Mouse CXCL1/KC ELISA kit (DY453-05) was purchased from R&D Systems (Minneapolis, MN). Collagenase (234155) was purchased from EMD Millipore (Billerica, MA). Anti-CD206 mouse Alexa Fluor 647 antibody (MCA2235A647T) was purchased from AbD Serotec (Raleigh, NC). Anti-Ly-6G (Gr-1) mouse PE antibody (553128) was purchased from BD Pharmingen (San Jose, CA). Blood agar base (70133), Hemin (H9039), and b-Nicotinamide Adenine Dinucleotide hydrate (NAD) (N3014) were purchased from Sigma (St. Louis, MO). Brain Heart Infusion (237500) and anti-CD80 mouse PerCP-Cy5.5 antibody (560526) were purchased from BD Bioscience (San Jose, CA). PGE2 and AT-RvD1 (7S,8R,17R-trihydroxy-4Z,9E,11E,13Z,15E, 19Z-docosahexaenoic acid) were purchased from Cayman Chemical (Ann Arbor, MI). Secondary Western blot antibodies (115-035-146, 111-035-144) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). PBS (14200-075) was purchased from Gibco (Waltham, MA).
Croasdell A., Lacy S.H., Thatcher T.H., Sime P.J, & Phipps R.P. (2016). Resolvin D1 dampens pulmonary inflammation and promotes clearance of Nontypeable Haemophilus influenzae. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2742-2752.
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3

Multiplexed Cytokine Analysis in Mice

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Mouse CXCL-1 (KC) ELISA Kit was purchased from R&D Systems (Minneapolis, MN, United States). Mouse MCP-1 and IL-6 ELISA kits were purchased from eBioscience (San Diego, CA, United States). Ear tissue and serum cytokine levels were determined according to the manufacturer’s instructions from 5–8 mice per treatment group from 4 independent experiments.
Wu B.C., Skovbakke S.L., Masoudi H., Hancock R.E, & Franzyk H. (2020). In vivo Anti-inflammatory Activity of Lipidated Peptidomimetics Pam-(Lys-βNspe)6-NH2 and Lau-(Lys-βNspe)6-NH2 Against PMA-Induced Acute Inflammation. Frontiers in Immunology, 11, 2102.
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4

Cytokine Profiling in Cell Culture

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Cells were cultured for 24 or 72 h. The cytokine concentrations in the cell supernatants were measured via ELISAs or human cytokine array, according to the manufacturer’s instructions. Infinite® M1000 Pro (Tecan) was used to measure absorbance. The following ELISA kits were used in this study: mouse MCP-1/CCL2 ELISA kit (88-7391-22, Invitrogen), mouse CXCL1/KC ELISA kit (DY453, R&D Systems), human CXCL1/GRO alpha ELISA kit (DY275, R&D Systems), and human CXCL8 ELISA kit (88-8086-88, Invitrogen). The human cytokine array (ARY005) was from R&D Systems (Minneapolis, MN).
Yang Z., Xu G., Wang B., Liu Y., Zhang L., Jing T., Tang M., Xu X., Jiao K., Xiang L., Fu Y., Tang D., Zhang X., Jin W., Zhuang G., Zhao X, & Liu Y. (2021). USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade. Nature Communications, 12, 4852.
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5

Quantifying Lung CXCL1 Levels in Mice

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Following blood withdrawal, the animals were perfused with 20 ml PBS via the inferior caval vein. The lungs were then removed and snap-frozen in liquid nitrogen. Lung tissue was homogenized in a protein lysis buffer at 4°C, followed by centrifugation for 30 min at 4°C and 20,000 × g. Subsequently, supernatants were stored at −80°C. CXCL1 concentrations in the lungs were determined using a Mouse CXCL1/KC ELISA kit from R&D Systems according to the manufacturer's instructions (Wiesbaden-Nordenstadt). ELISA was performed using an Infinite M200 microplate reader (Tecan Deutschland GmbH).
Vollrath J.T., Stoermann P., Becker N., Wutzler S., Hildebrand F., Marzi I, & Relja B. (2020). Early local neutralization of CC16 in sepsis-induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival. International Journal of Molecular Medicine, 46(6), 2207-2215.
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