Hp 5ms capillary column

Leaves of Ocimum basilicum L. were purchased from local markets and authenticated in the herbarium of Faculty of Science, Cairo University and National Research Center, Egypt.
Extraction of Essential Oil: 1 kg of Ocimum basilicum L. leaves were subjected to hydrodistillation. The volatile oil then collected and dried in desiccators over anhydrous CaSO₄. The volatile oil sample was kept in dark bottle for GC and GC/MS (mass spectrometry) analyses.
Analysis of the Essential Oil GC: The essential oil was analysed on Perkin-Elmer gas chromatograph model 8700, equipped with HP-5MS capillary column (30 m × 0.25 mm, film thickness 0.25 μm)programming from 80ºC (3 min) to 220ºC (10 min). Helium as a carrier gas (1.5 ml/min). Injection in split mode (1:100).
GC/MS: The essential oil was also analysed on Agilent-Technologies 6890N network gas chromatographic (GC) system (Little Falls, California, USA), equipped with HP-5 MS capillary column (30 m × 0.25 mm, film thickness 0.25 μm) programming from 80ºC (3 min) to 220ºC (10 min). Helium as a carrier gas (1.5 ml/min). Injection in split mode (1:100) [13 (link), 14 ].
Identification of Compounds: Compounds were identified by computer search using their mass spectra either with the known components or published spectra and by comparison of their retention indices with those of known compounds [15 , 16 ].

Analytical gas chromatography was carried out on a Perkin-Elmer Sigma 115 gas chromatograph fitted with a HP-5 MS capillary column (30 m × 0.25 mm), 0.25 μm film thickness. Analysis was also run by using a fused silica HP Innowax polyethylenglycol capillary column (50 m × 0.20 mm), 0.20 μm thick film. Gas chromatography analysis has been performed as described in detail previously [21 (link)]. Identification of the compounds and components relative percentages was carried out as described in [21 (link)].