Differential quik stain

Manufactured by Polysciences

Sourced in Japan

The Differential Quik Stain is a multi-step staining procedure used to differentiate between various types of cells, such as red blood cells, white blood cells, and platelets, in blood smear samples. It provides a rapid and reliable way to perform differential cell counts for clinical analysis and research applications.

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Lab products found in correlation

Matrigel, by BD (2 mentions) Formalin, by Merck Group (1 mentions) Matrigel, by Merck Group (1 mentions) Boyden chamber, by Corning (1 mentions)

Close spelling variants of this product

Differential Quik Stain Kit Differential Quik III Stain Kit

2 protocols using differential quik stain

Matrigel Invasion Assay for Quantifying Cell Invasion

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After indicate treatments, equal cell numbers of the Fadu (1×10⁶ cells/chamber) or Tu686 (0.5×10⁶ cells/chamber) cell lines for each condition were placed in deprivation media in the upper chamber (8.0 μ m pores, Corning, Inc) coated with Matrigel (BD Biosciences). Deprivation media was placed in the lower chamber. Cells were allowed to invade for 48 h. The cells remaining in the upper chamber were removed from the upper chamber by a cotton swab. After fixation with 4 % formalin and staining with Differential Quik Stain (Polysciences, Inc), invaded cells were acquired on an inverted Olympus IX81 microscope (Olympus, Tokyo, Japan). To evaluate the invasive ability, the invaded cells were counted.

Wu P., Wu H., Tang Y., Luo S., Fang X., Xie C., He J., Zhao S., Wang X., Xu J., Chen X., Li D., Yang H, & Wang J. (2017). Whole-exome sequencing reveals novel mutations and epigenetic regulation in hypopharyngeal carcinoma. Oncotarget, 8(49), 85326-85340.

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Prolactin Modulates Cell Invasion

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Equal numbers of T47D and TMX2-28 cells (5x10⁵) were placed in deprivation media with or without inhibitors (0.1μM PP1, 50μM AG490) in the upper chamber of a Boyden chamber (Corning Inc.) coated with Matrigel (BD Biosciences). Deprivation media with or without 500ng/ml PRL was placed in the lower chamber. After 48 hours, cells from five separate fields that had invaded the Matrigel were counted after fixation with 4% formalin (Sigma) and staining with Differential Quik Stain (Polysciences, Inc).

Hammer A., Laghate S, & Diakonova M. (2015). Src tyrosyl phosphorylates cortactin in response to prolactin. Biochemical and biophysical research communications, 463(4), 644-649.

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