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A96606

Manufactured by Merck Group
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A96606 is a piece of laboratory equipment manufactured by Merck Group. It is designed for general laboratory use, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Pluronic f 127, by Thermo Fisher Scientific (4 mentions) Fluozin 3 am, by Thermo Fisher Scientific (2 mentions) S7400, by Merck Group (2 mentions) P4413, by Merck Group (2 mentions)

2 protocols using a96606

1

Zinc Regulation in Cellular Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with 500 µM sodium arsenite (ARS) (NaAsO2; Sigma-Aldrich # S7400) for 1 hr prior to collection or fixing onto cover glasses. Cells were treated with indicated concentrations of TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine) (10, 20, or 40 µM) (Sigma-Aldrich # P4413), azodicarbonamide (ADA; 50µM, Sigma-Aldrich #A96606) or 2,2′-dithiobisbenzamide-1 (DIBA-1; 100µM, Sigma-Aldrich #PZ0634) for 1hr prior to cell harvest. For visualization of Zn2+, cells were loaded with 1 µM FluoZin-3 AM (Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Thermo Fisher Scientific # P3000MP) for 40 min, washed, and given fresh media. NC-LLPSs were treated with 500 µM ARS, 20 µM TPEN, or 3.5% 1,6-hexanediol (HEX) (Sigma-Aldrich #240117). The HIV-1 PR inhibitor saquinavir (SAQ) was obtained from the Division of AIDS, NIH through the NIH AIDS Research Reference and Reagent Program, and was used from the time of transfection to the time of collection.
Monette A., Niu M., Chen L., Rao S., James Gorelick R, & John Mouland A. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell reports, 31(3), 107520.
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2

Zinc Regulation in Cellular Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with 500 µM sodium arsenite (ARS) (NaAsO2; Sigma-Aldrich # S7400) for 1 hr prior to collection or fixing onto cover glasses. Cells were treated with indicated concentrations of TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine) (10, 20, or 40 µM) (Sigma-Aldrich # P4413), azodicarbonamide (ADA; 50µM, Sigma-Aldrich #A96606) or 2,2′-dithiobisbenzamide-1 (DIBA-1; 100µM, Sigma-Aldrich #PZ0634) for 1hr prior to cell harvest. For visualization of Zn2+, cells were loaded with 1 µM FluoZin-3 AM (Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Thermo Fisher Scientific # P3000MP) for 40 min, washed, and given fresh media. NC-LLPSs were treated with 500 µM ARS, 20 µM TPEN, or 3.5% 1,6-hexanediol (HEX) (Sigma-Aldrich #240117). The HIV-1 PR inhibitor saquinavir (SAQ) was obtained from the Division of AIDS, NIH through the NIH AIDS Research Reference and Reagent Program, and was used from the time of transfection to the time of collection.
Monette A., Niu M., Chen L., Rao S., James Gorelick R, & John Mouland A. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell reports, 31(3), 107520.
+ Open protocol
+ Expand

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