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Cp achromat 10 0.25 air

Manufactured by Zeiss
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The CP-Achromat 10 × 0.25 air is a high-quality microscope objective lens manufactured by Zeiss. It is designed to provide a magnification of 10× with a numerical aperture of 0.25 when used with air as the immersion medium. The lens is made using the achromatic design, which helps to reduce chromatic aberrations and provide improved image quality.

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Lab products found in correlation

Axon multiclamp 700b amplifier, by Molecular Devices (2 mentions) Pclamp software, by Molecular Devices (2 mentions) Axioskop 2 fs plus microscope, by Zeiss (2 mentions) Matlab, by Wavemetrics (2 mentions) Lumplanfl 60 1.0 na water immersion, by Olympus (2 mentions) Lb ls 17 lamp, by Sutter Instruments (2 mentions) Igor pro, by Wavemetrics (2 mentions)

2 protocols using cp achromat 10 0.25 air

1

Patch Clamp Electrophysiology Technique

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Manual patch clamp experiments were performed on an Axioskop 2 FS Plus microscope (Zeiss) with CP-Achromat 10 × 0.25 air (Zeiss) and LumplanFL 60 ×1.0 NA water immersion (Olympus) objectives, and Sutter MPC-200 multi manipulator system with ROE-200 controller. The rig was equipped with a Sutter LB-LS/17 lamp for fluorescence and DIC optics. An Axon Multiclamp 700B amplifier, Axon Digidata 1440A A/D converter and pClamp software (Molecular Devices, Sunnyvale, CA) were used to acquire electrophysiological signals. Currents were normalized to cell size using whole-cell capacitance upon cell break-in, and leak currents were subtracted using a P/4 protocol. Data were analyzed using Microsoft Excel, MATLAB, Igor Pro (WaveMetrics, Lake Oswego, OR), and GraphPad Prism. Pooled data are presented as either bar graphs ±SEM overlaid with individual data points or in tabular format ±SEM.
Murphy J.G., Gutzmann J.J., Lin L., Hu J., Petralia R.S., Wang Y.X, & Hoffman D.A. (2022). R-type voltage-gated Ca2+ channels mediate A-type K+ current regulation of synaptic input in hippocampal dendrites. Cell reports, 38(3), 110264.
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2

Patch Clamp Electrophysiology Technique

Check if the same lab product or an alternative is used in the 5 most similar protocols
Manual patch clamp experiments were performed on an Axioskop 2 FS Plus microscope (Zeiss) with CP-Achromat 10 × 0.25 air (Zeiss) and LumplanFL 60 ×1.0 NA water immersion (Olympus) objectives, and Sutter MPC-200 multi manipulator system with ROE-200 controller. The rig was equipped with a Sutter LB-LS/17 lamp for fluorescence and DIC optics. An Axon Multiclamp 700B amplifier, Axon Digidata 1440A A/D converter and pClamp software (Molecular Devices, Sunnyvale, CA) were used to acquire electrophysiological signals. Currents were normalized to cell size using whole-cell capacitance upon cell break-in, and leak currents were subtracted using a P/4 protocol. Data were analyzed using Microsoft Excel, MATLAB, Igor Pro (WaveMetrics, Lake Oswego, OR), and GraphPad Prism. Pooled data are presented as either bar graphs ±SEM overlaid with individual data points or in tabular format ±SEM.
Wood G.E., Dutro S.M, & Totten P.A. (1999). Target Cell Range of Haemophilus ducreyi Hemolysin and Its Involvement in Invasion of Human Epithelial Cells. Infection and Immunity, 67(8), 3740-3749.
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