As 55593

The dissected trunk regions of zebrafish embryos were collected for extracting protein. Western blotting was performed^{60 (link)} with 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The proteins were then transferred into a nitrocellulose membrane and then membranes were blocked by nonfat milk. After blocking, the nitrocellulose membranes were then incubated using anti-Fsd1 antibody (Ab) (1:200, monoclonal mouse or polyclonal rabbit anti-FSD1 antibody was generated using a glutathione-S-transferase fusion protein containing the full-length FSD1, which were expressed in E. coli and then were purified to homogeneity as the antigen. The monoclonal mouse Ab was used in human RPE-1 cells, while the polyclonal rabbit Ab was used in zebrafish experiments), anti-Runx1 Ab (1:200, AS-55593, Ana Spec), anti-NICD Ab (1:400, ab83232, Abcam, used in zebrafish), anti-NICD Ab (1:1000, ab8925, Abcam, used in human cells), mouse anti-β-actin (1:1000, sc47778, Santa Cruz Biotechnology, used in human cells), and anti-β-actin Ab (1:2000, 4967, Cell Signaling). The figures for western blotting were collected by Imagequant LAS 4000 (The uncropped scans of blots are listed in the Supplementary Figure 12).

Protein was extracted from the dissected trunk regions of 36 hpf zebrafish embryos. Western blotting was performed as previously reported (Zhang et al., 2014 (link)) using the following antibodies: anti-β-Actin antibody (4967, Cell Signaling Technology, 1:1000), anti-Notch1 antibody (ab65297, Abcam, 1:100), anti-Dnmt1 antibody (24206, Proteintech, 1:300), anti-Runx1 antibody (AS-55593, Ana Spec, 1:200).