Bicoll

Manufactured by Harvard Bioscience

Sourced in Germany

Bicoll is a laboratory equipment product designed for protein chromatography applications. It provides a robust and reliable platform for the separation, purification, and analysis of various biomolecules. The core function of Bicoll is to facilitate the efficient separation and isolation of target proteins from complex biological samples.

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Lab products found in correlation

Human cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Pan monocyte isolation kit, by Miltenyi Biotec (1 mentions) Fetal calf serum (fcs), by GE Healthcare (1 mentions) Dimethyl sulfoxide (dmso), by Carl Roth (1 mentions)

Spelling variants of this product

Bicoll Separating Solution (6 citations) Bicoll Seperation (3 citations)

4 protocols using bicoll

Isolation of CD4+ T Cells from Human PBMCs

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Human peripheral blood samples were obtained from the Department of Transfusion Medicine at the University Hospital Würzburg, and use of the samples complied with the approval of the University Hospital of Würzburg Ethical Committee. PBMCs were isolated by standard Ficoll gradient centrifugation (Bicoll; Biochrom). CD4⁺ T cells were enriched using a human CD4⁺ T cell isolation kit (Miltenyi Biotec).

Chopra M., Biehl M., Steinfatt T., Brandl A., Kums J., Amich J., Vaeth M., Kuen J., Holtappels R., Podlech J., Mottok A., Kraus S., Jordán-Garrote A.L., Bäuerlein C.A., Brede C., Ribechini E., Fick A., Seher A., Polz J., Ottmüller K.J., Baker J., Nishikii H., Ritz M., Mattenheimer K., Schwinn S., Winter T., Schäfer V., Krappmann S., Einsele H., Müller T.D., Reddehase M.J., Lutz M.B., Männel D.N., Berberich-Siebelt F., Wajant H, & Beilhack A. (2016). Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. The Journal of Experimental Medicine, 213(9), 1881-1900.

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Isolation of Primary Human Monocytes

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Human primary monocytes were isolated out of 65 ml blood taken from antecubital vein of female donors (n = 4), by density gradient centrifugation, using Bicoll (Biochrom) to obtain peripheral blood mononuclear cells (PBMCs), which were subsequently subjected to Pan Monocyte Isolation Kit (130-096-537, MACS, Miltenyi Biotec), according to the manufacturer’s protocol. The mean purity of enriched monocytes was 91.5(±0.9)% and was evaluated on a Canto II multicolor flow cytometry platform (BD). Monocytes were identified after live/dead discrimination and doublet exclusion as being CD3⁻ CD19⁻ CD14⁺ using fluorophore-conjugated monoclonal antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany).

Nonn O., Güttler J., Forstner D., Maninger S., Zadora J., Balogh A., Frolova A., Glasner A., Herse F, & Gauster M. (2019). Placental CX3CL1 is Deregulated by Angiotensin II and Contributes to a Pro-Inflammatory Trophoblast-Monocyte Interaction. International Journal of Molecular Sciences, 20(3), 641.

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Profiling Immune Cell Dynamics in Aging

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Peripheral blood mononuclear cells (PBMC) were purified from blood with the gradient method (Bicoll, 1.077 g/ml, Biochrom, Berlin, Germany). The healthy human blood donors were separated into young (23-30 years) and old (60-68 years) donors (Supplementary Table 1). These two age groups were selected based on published evidence, which shows that the two selected ages are distinct enough for the current study (38 (link)). The blood was provided by the blood bank of the University Hospital in Halle (Saale), Germany, upon donor consent. In total, blood from 32 healthy donors (equal numbers of women and men) was analyzed. Due to the limited numbers of cells that can be isolated, these donors were split into two cohorts for each one cell population,

Toma G., Lemnian I.M., Karapetian E., Grosse I, & Seliger B. (2022). Transcriptional Analysis of Total CD8+ T Cells and CD8+CD45RA- Memory T Cells From Young and Old Healthy Blood Donors. Frontiers in Immunology, 13, 806906.

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Characterizing T-cell Subsets in PBMCs

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The frequencies of various T-cell subsets in peripheral blood mononuclear cells (PBMCs) were determined at the various predefined time points and in the event of a flare. PBMCs were isolated from heparin-treated blood by Bicoll (Biochrom, Germany) density gradient centrifugation (Biochrom AG, Berlin, Germany). Isolated cells were washed with buffer and were either analyzed immediately by flow cytometry or cryopreserved in medium containing 10% fetal calf serum (FCS; PAA Laboratories GmbH, Pasching, Austria) and 10% dimethyl sulfoxide (DMSO; Carl Roth GmbH, Karlsruhe, Germany).

Langhorst J., Frede A., Knott M., Pastille E., Buer J., Dobos G.J, & Westendorf A.M. (2014). Distinct Kinetics in the Frequency of Peripheral CD4+ T Cells in Patients with Ulcerative Colitis Experiencing a Flare during Treatment with Mesalazine or with a Herbal Preparation of Myrrh, Chamomile, and Coffee Charcoal. PLoS ONE, 9(8), e104257.

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