Seq chip

Manufactured by Abcam

Seq-ChIP is a high-throughput sequencing-based chromatin immunoprecipitation (ChIP-seq) assay. It is used to identify genome-wide binding sites of DNA-binding proteins, such as transcription factors and histone modifications.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) α 32p dctp, by PerkinElmer (1 mentions) Bile acid, by Merck Group (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) C myc, by Thermo Fisher Scientific (1 mentions) Interleukin 6 il 6, by Merck Group (1 mentions) C myc, by Abcam (1 mentions) β actin, by Abcam (1 mentions) C maf, by Abcam (1 mentions) Matα1, by Abcam (1 mentions)

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CHIP-seq high sensitivity kit (2 citations)

2 protocols using seq chip

Western Blot and ChIP-Seq Workflow

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α-³²P dCTP (6,000 Ci/mmol) was purchased from PerkinElmer (Boston, MA). Antibodies used for Western, ChIP, Seq-ChIP, and immunohistochemistry (IHC) to PHB1, MATα1, c-MYC, MAFG, c-MAF, β-ACTIN, and IgG were purchased from Abcam (Cambridge, MA). Lipofectamine 2000 and RNAi-Max were purchased from ThermoFisher (Carlsbad, CA). siRNAs to PHB1 (Cat# 4392422), c-MYC (5′-CGAUUCCUUCUAACAGAAtt-3′), MAFG (5′-CGGACUAGAGAGAGUUGCGtt-3′), c-MAF (5′-GCAUCGUGUACUUACCAGUtt-3′) and MAT1A (5′-GCACAACGAAGACAUCACGtt-3′) were purchased from ThermoFisher. Bile acids and interleukin-6 (IL-6) were from Sigma (St. Louis, MS).

Fan W., Yang H., Liu T., Wang J., Li T.W., Mavila N., Tang Y., Yang J., Peng H., Tu J., Annamalai A., Noureddin M., Krishnan A., Gores G.J., Martínez-Chantar M., Mato J.M, & Lu S.C. (2017). Prohibitin 1 Suppresses Liver Cancers Tumorigenesis in Mice and Human Hepatocellular and Cholangiocarcinoma cells. Hepatology (Baltimore, Md.), 65(4), 1249-1266.

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Antibody-Based Experimental Procedures

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Antibodies used for western blotting, immunohistochemistry (IHC), chromatin immunoprecipitation (ChIP), and sequential-ChIP (Seq-ChIP) for FOXM1, MATα1 (protein encoded by MAT1A), p65, p50, prohibitin 1 (PHB1), hepatocyte nuclear factor 4 (HNF4), alpha-fetoprotein (AFP), cytochrome P450 2E1 (CYP2E1), β-ACTIN, and IgG were purchased from Abcam (Cambridge, MA). MAT1A^{(18 (link))} and RELA (p65)^{(19 (link))} expression vectors were previously described. Please see Supplemental Experimental Procedures for sources of other reagents.

Li Y., Lu L., Tu J., Zhang J., Xiong T., Fan W., Wang J., Li M., Chen Y., Steggerda J., Peng H., Chen Y., Li T.W., Zhou Z.G., Mato J.M., Seki E., Liu T., Yang H, & Lu S.C. (2020). Reciprocal Regulation Between Forkhead Box M1/NF-kB and Methionine Adenosyltransferase 1A Drives Liver Cancer. Hepatology (Baltimore, Md.), 72(5), 1682-1700.

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