Sirnas
SiRNAs are a class of small, double-stranded RNA molecules that play a role in the RNA interference (RNAi) pathway. They function by targeting and silencing specific mRNA molecules, thereby regulating gene expression.
Lab products found in correlation
11 protocols using sirnas
siRNA Transfection in CHO-K1 Cells
siRNA Transfection by Electroporation
Silencing PI4K Enzymes in HUVECs
Transient RNAi Silencing in Organism
Repeated siRNA Transfection of Cells
Transient Transfection of RAW264.7 Cells with siRNA Targeting cPLA2γ
Antibodies and siRNA for p53 Pathway
siRNAs were purchased from Eurofins (Ebersberg, Germany). Sequences are available on request. siRNA transfections were performed using Macsfectin (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer's instructions.
Silencing of TEAD, YAP/TAZ, GTSE1 and E2F1 in Breast Cancer Cells
The AllStars negative control siRNA (Qiagen, 1027281) was used as negative control.
Lipofectamine RNAi-MAX (Invitrogen) was used for siRNAs transfections in antibiotic-free medium according to the manufacturer's instructions. Cells were transfected in reverse using 20nM siRNA. The TEAD1/3/4 and YAP/TAZ silencing was performed by transfecting the siRNAs in MDA-MB-231 and MDA-MB-157 cell lines for 72 hours.
GTSE1 and E2F1 silencing was carried out transfecting cells with siRNAs for 48 hours.
Oligonucleotide Synthesis and Labeling
Cen-RNA Depletion by ASOs and siRNAs
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