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Cc1 solution

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The CC1 solution is a laboratory reagent used for tissue preparation and pretreatment in various applications. It is designed to facilitate the processing and analysis of biological samples.

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Lab products found in correlation

Ez prep solution, by Roche (6 mentions) Benchmark xt, by Roche (5 mentions) Ez prep, by Roche (4 mentions) Hematoxylin 2, by Roche (4 mentions) Ventana discovery xt, by Roche (4 mentions) Iview dab detection kit, by Roche (3 mentions) Optiview dab detection kit, by Roche (3 mentions) Antibody diluent solution, by Zytomed Systems (3 mentions) Goat serum, by Dianova (3 mentions) Hematoxylin 1, by Roche (2 mentions) Optiview dab ihc detection kit, by Roche (2 mentions) Nch 38, by Agilent Technologies (2 mentions) Ultraview universal dab detection system, by Roche (2 mentions) Ventana benchmark xt, by Roche (2 mentions) Discovery xt, by Roche (2 mentions) Tissuetek glove mounting media, by Sakura Finetek (2 mentions) Ultraview universal dab detection kit, by Roche (2 mentions) Ventana benchmark xt machine, by Roche (2 mentions) Benchmark ultra, by Roche (2 mentions) Ventana benchmark ultra, by Roche (2 mentions)

25 protocols using cc1 solution

1

Immunohistochemical Detection of ROS1 Protein

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Protein expression of ROS1 was detected by immunohistochemical staining in the Korean cohort, and it was not performed in the Singapore cohort due to lack of unstained slides. Tissue microarray sections were stained using the Ventana automated immunostainer BenchMark XT (Ventana Medical Systems, Tucson, AZ). The slides were dried at 60°C for 1 hour and deparaffinized using EZ Prep (Ventana Medical Systems) at 75°C for 4 minutes. Cell conditioning was performed using CC1 solution (Ventana Medical Systems) at 100°C for 8 minutes. ROS1 antibody (rabbit monoclonal, clone D4D6, Cell Signaling Technology, Danvers, MA) was diluted to 1:10, followed by treatment, and incubation at 37°C for 2 hours. Signals were detected using the OptiView DAB IHC Detection Kit (Ventana Medical Systems). Counterstaining was performed using Hematoxylin I (Ventana Medical Systems) for 4 minutes at room temperature.
Lim S.M., Yoo J.E., Lim K.H., Meng Tai D.W., Cho B.C, & Park Y.N. (2016). Rare Incidence of ROS1 Rearrangement in Cholangiocarcinoma. Cancer Research and Treatment : Official Journal of Korean Cancer Association, 49(1), 185-192.
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2

Immunohistochemical Evaluation of Vimentin and E-Cadherin

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Immunohistochemical staining was performed on 5–μm sections of formalin-fixed, paraffin-embedded tissue using antibody to Vimentin (NCL-L-VIM-V9; Novocasrtra, UK) and E-Cadherin (NCH-38; Dako, CA USA). The visualization system used was BenchMark XT with heat-induced epitope retrieval (CC1 solution, Ventana) and iView DAB detection kit (Ventana, Tucson, AZ).
Saygideger Y., Avci A., Bagir E., Saygıdeğer Demir B., Sezan A., Ekici M., Baydar O, & Erkin Ö.C. (2022). Slug and Vimentin downregulation at the metastatic site is associated with Skip-N2 metastasis of lung adenocarcinoma. Discover. Oncology, 13, 7.
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3

Immunohistochemical Delineation of Hippocampal Subregions

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Formalin-fixed, paraffin-embedded blocks of autopsy brain tissue (4 Km thick) were deparaffinized. Immunohistochemistry for ZNT3 was performed in some cases to define the border between the CA3 and the CA2. For antigen retrieval, the sections were placed in CC1 solution (Ventana Medical Systems, Tucson, AZ) and heated at 95°C for 30 minutes. The sections were exposed to a rabbit polyclonal antibody to human ZNT3 (BMP094; MBL Corp., Woburn, MA) at a dilution of 1:2000 for 32 minutes. The signal was developed with the ultraView Universal DAB Detection system (Ventana Medical Systems). The immunostaining procedure was performed using the Benchmark Ultra automated stainer (Ventana Medical Systems).
Immunohistochemistry for choline acetyltransferase (ChAT) was performed in some cases to delineate the border between the CA2 and the CA1. For antigen retrieval, the sections were placed in a Pascal programmable pressure cooker (Dako, Carpinteria, CA) containing Reveal Decloaker solution (Biocare Medical, Concord, CA), with the target temperature and time set to 125°C and 30 seconds, respectively. The sections were exposed to a rabbit polyclonal antibody to ChAT (AB143; Millipore, Billerica, MA) at a dilution of 1:400 for 32 minutes. The signal was developed with the Envision Plus Rabbit detection system (Dako). The immunostaining procedure was performed using the Autostainer Plus (Dako).
Hatanpaa K.J., Raisanen J.M., Herndon E., Burns D.K., Foong C., Habib A.A, & White CL I.I.I. (2014). Hippocampal Sclerosis in Dementia, Epilepsy, and Ischemic Injury: Differential Vulnerability of Hippocampal Subfields. Journal of neuropathology and experimental neurology, 73(2), 136-142.
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4

Immunohistochemical Analysis of FFPE Tissues

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Formalin-fixed paraffin-embedded (FFPE) tissues were stained using the Ventana BenchMark XT automated immunostainer (Ventana Medical Systems, Tucson, AZ, USA), according to the manufacturer’s instructions. Thereafter, the slides were dried at 60 °C for 1 h and deparaffinized using EZ Prep (Ventana Medical Systems, Tucson, AZ, USA) at 75 °C for 4 min. Cell conditioning was performed using the CC1 solution (Ventana Medical Systems, Tucson, AZ, USA) at 100 °C for 64 min. Subsequently, the slides were incubated with ID-1, ID-2, ID-3, and ID-4 rabbit polyclonal antibodies (Abcam, Cambridge, UK) and E2A rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted to 1:50 at 37 °C for 32 min. The signals were detected using the OptiView DAB IHC Detection Kit (Ventana Medical Systems, Tucson, AZ, USA). Counterstaining was performed using hematoxylin I (Ventana Medical Systems, Tucson, AZ, USA) for 4 min at room temperature.
Lee Y.J., Nam E.J., Kim S., Kim Y.T., Itkin-Ansari P, & Kim S.W. (2022). Expression Profiles of ID and E2A in Ovarian Cancer and Suppression of Ovarian Cancer by the E2A Isoform E47. Cancers, 14(12), 2903.
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5

Automated Immunohistochemistry Assay Protocol

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Immunohistochemistry assays were performed on a VENTANA Discovery XT automated staining instrument according to the manufacturer's instructions. Slides were de-paraffinized using EZprep solution (Ventana Medical Systems, Inc., Tucson, AZ) for 30 minutes at 75°C. Epitope retrieval was accomplished on the automated stainer with CC1 solution (Ventana Medical Systems, Inc., Tucson, AZ) for 64 minutes at 95°C. Antibodies obtained from Cell Signalling Technologies or Epitomics were first titered over a range of concentrations to provide the optimum ratio of specific staining to background staining. Once titers were set, antibodies were transferred with diluent to user fillable dispensers for use on the automated stainer. Slides were developed using the Optiview DAB detection kit (Ventana Medical Systems, Inc., Tucson, AZ). Briefly, steps included inhibitor for 8 minutes, linker for 8 minutes, multimer for 12 minutes, DAB/peroxide for 8 minutes and copper for 4 minutes. Slides were then counterstained with hematoxylin II for 8 minutes (Ventana Medical Systems, Inc., Tucson, AZ). Antibodies, clones, and titers are listed in Table 1. Antibody titers were determined for each antibody using positive and negative control tissues following the manufacturer's instructions.
Theiss A.P., Chafin D., Bauer D.R., Grogan T.M, & Baird G.S. (2014). Immunohistochemistry of Colorectal Cancer Biomarker Phosphorylation Requires Controlled Tissue Fixation. PLoS ONE, 9(11), e113608.
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6

Automated Immunohistochemistry Assay Protocol

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Immunohistochemistry assays were performed on a VENTANA Discovery XT automated staining instrument (Ventana Medical Systems, Tucson, AZ, USA). Slides were de-paraffinized using EZ Prep solution (Ventana Medical Systems) for 30 min at 75 °C. Epitope retrieval with CC1 solution (Ventana Medical Systems) was performed for 64 min at 95 °C. Antibodies were first titered over a range of concentrations to provide the optimum ratio of specific staining to background staining. Once titers were set, antibodies were transferred with diluent to user-fillable dispensers for use on the automated stainer. Anti-ALDH1L1 (ab175198, 1:50) and cytokeratin 19 (ab52625, 1:1000) antibodies were acquired from Cell Signaling Technology (Danvers, MA, USA). Slides were developed using the OptiView DAB detection kit (Ventana Medical Systems). Briefly, samples were incubated with inhibitor for 8 min, linker for 8 min, multimer for 12 min, DAB/peroxide for 8 min, and copper for 4 min. The slides were then counterstained for 8 min with hematoxylin II (Ventana Medical Systems). Antibody titers were determined for each antibody using positive and negative control tissues, according to the manufacturer’s instructions.
Lee S.H., Jeon Y., Kang J.H., Jang H., Lee H, & Kim S.Y. (2020). The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer. Cancers, 12(6), 1382.
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7

Immunohistochemical Staining of Paraffin-Embedded Tissues

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Tissues were collected and fixed in 10% phosphate-buffered formaldehyde, dehydrated, and embedded in paraffin. Hematoxylin- and eosin- (H&E) stained sections were used for morphological evaluation purposes. Unstained sections were used for immunohistochemical (IHC) studies. IHC staining was performed on a VENTANA Discovery XT automated staining instrument (Ventana Medical Systems) using VENTANA reagents according to the manufacturer’s instructions. Slides were de-paraffinized using EZ Prep solution (Ventana Medical Systems, #950–102) for 16 min at 72 °C. Epitope retrieval was accomplished with CC1 solution (Ventana Medical Systems, cat #950–224) at 95–100 °C for 32 min. Primary antibodies were tittered with a TBS antibody diluent into user fillable dispensers for use on the automated stainer. Immune complex was detected using the Ventana OmniMap anti-Rabbit detection kit (#760-4311) and developed using the VENTANA ChromMap DAB detection kit (#760-159) according to the manufacturer’s instructions. Slides were counterstained with hematoxylin II (#790-2208) for 8 min, followed by Bluing reagent (#760-2037) for 4 min. The slides were then dehydrated with an ethanol series, cleared in xylene, and mounted. All slides were viewed with a Nikon Eclipse 50i microscope and photomicrographs were taken with an attached Nikon DS-Fi1 camera (Melville, NY, USA).
Makhov P., Fazliyeva R., Tufano A., Uzzo R.G., Cai K.Q., Serebriiskii I., Snyder N.W., Andrews A.J, & Kolenko V.M. (2022). Acetyl-CoA Counteracts the Inhibitory Effect of Antiandrogens on Androgen Receptor Signaling in Prostate Cancer Cells. Cancers, 14(23), 5900.
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8

Nestin Expression in Human Gliomas

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Nestin protein expression was evaluated on tissue microarrays by immunohistochemistry. Cores (1.0 mm) for tissue microarrays (TMAs) were punched using a tissue microarrayer (Beecher Instruments, Sun Prairie, WI). The donor blocks were formalin-fixed, paraffin-embedded tissue blocks of human gliomas. TMA sections of human gliomas were cut at 4 micron thickness and deparaffinized. For antigen retrieval, the sections were heated for 30 min at 98 C in CC1 Solution (Ventana Medical Systems, Tucson, AZ). The sections were exposed to a rabbit polyclonal antibody to human nestin (AB5986; Abcam) at a dilution of 1:1,000. The signal was developed with ultraView universal DAB detection system (Ventana). The immunostaining procedure was performed using the Benchmark XT automated stainer (Ventana).
Hatanpaa K.J., Hu T., Vemireddy V., Foong C., Raisanen J.M., Oliver D., Hiemenz M.C., Burns D.K., White CL I.I.I., Whitworth L.A., Mickey B., Stegner M., Habib A.A., Fink K., Maher E.A, & Bachoo R.M. (2014). High expression of the stem cell marker nestin is an adverse prognostic factor in WHO grade II-III astrocytomas and oligoastrocytomas. Journal of neuro-oncology, 117(1), 183-189.
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9

Immunohistochemical Staining of Phospho-AXL

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Immunohistochemical staining of formalin-fixed, paraffin-embedded (FFPE) tissue sections (4 μm-thick) was performed on a VENTANA Benchmark XT automated staining instrument according to the manufacturer's instructions. Slides were de-paraffinized using EZ prep solution (Ventana Medical Systems, Tucson, AZ) for 30 minutes at 75°C. Antigen retrieval was accomplished on the automated stainer using CC1 solution (Ventana Medical Systems, Tucson, AZ) for 60 minutes at 95°C. Briefly, the anti-phospho-AXL antibody (Human Phospho-AXL (Y779) monoclonal mouse IgG Clone 713610, R & D Systems, dilution 1:50) was applied and developed using the iVIEW DAB Detection Kit (Ventana Medical Systems). All slides were then counterstained with hematoxylin for 4 minutes.
Onken J., Torka R., Korsing S., Radke J., Krementeskaia I., Nieminen M., Bai X., Ullrich A., Heppner F, & Vajkoczy P. (2016). Inhibiting receptor tyrosine kinase AXL with small molecule inhibitor BMS-777607 reduces glioblastoma growth, migration, and invasion in vitro and in vivo. Oncotarget, 7(9), 9876-9889.
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10

Immunohistochemical Analysis of PrP Expression

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Sections were stained using a Ventana Benchmark XT (Ventana). Deparaffinised sections were incubated for 30–60 min in CC1 solution (Ventana) for antigen retrieval. Primary antibodies were diluted in 5% goat serum (Dianova), 45% Tris-buffered saline pH 7.6 (TBS) and 0, 1% Triton X-100 in antibody diluent solution (Zytomed). Sections were then incubated with primary antibody for 1 h (see supplemental list). Anti-mouse histofine Simple Stain MAX PO Universal immunoperoxidase polymer (Nichirei Biosciences) were used as secondary antibody. Detection of secondary antibodies was performed with an ultraview universal DAB detection kit from Ventana with appropriate counterstaining and sections were cover-slipped using TissueTek glove mounting media (Sakura Finetek). To allow comparability for each analysis, sections of Tg(PrPΔ214–229) mice and controls were stained in one machine run.
For PrPSc staining, samples were treated for 30 min in Peroxidase-Blocking buffer (0, 3% H2O2) and 30 min in 98% formic acid. After washing with water, samples were autoclaved for 5 min at 121 °C in citrate buffer pH 6. The protocol was then followed as described above with the additional step of PK digestion followed by incubation of SAF84 antibody.
Puig B., Altmeppen H.C., Ulbrich S., Linsenmeier L., Krasemann S., Chakroun K., Acevedo-Morantes C.Y., Wille H., Tatzelt J, & Glatzel M. (2016). Secretory pathway retention of mutant prion protein induces p38-MAPK activation and lethal disease in mice. Scientific Reports, 6, 24970.
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