Anti lxrβ

Immunoblotting was carried out as described previously^{5 (link)}. Proteins were separated on SDS-PAGE gels, electro-transferred onto polyvinylidene difluoride membranes and incubated overnight at 4 °C with the following antibodies: anti-Atg3, anti-Atg5 (D1G9), anti-Atg7 (D12B11), anti-Beclin (D40C5), anti-caspase-3 (Cell Signaling), anti-Bcl-2 (Millipore), anti-Bax (Millipore), anti-LC3 (Sigma-Aldrich), anti-LXRα (SantaCruz), anti-LXRβ (SantaCruz), anti-Nur77 (Active motif), anti-NOR1 (R&D systems), anti-PARP (Cell Signaling), anti-Vps34 (Cell Signaling), or anti-Actin (Millipore). For visualization, an ECL plus kit (Amersham Biosciences) was used, and chemiluminescence was measured by autoradiography (Supplementary Fig. 9). Specific bands were quantified with ImageQuant software.

Recombinant mouse Gas6 was purchased from R&D Systems (Minneapolis, MN, USA). Wortmanin and LY294002 were purchased from Sigma-Aldrich (St. Louis, MO, USA). MK220 and T0901317 were obtained from Cayman Chemical (Ann Arbor, MI, USA). The Gene-Specific Relative RT-PCR kit was obtained from Invitrogen (Carlsbad, CA, USA). M-MLV reverse transcriptase was from Enzynomics (Hanam, Korea). The following antibodies were used: anti-LXRβ, anti-ABCG1, anti-Aim, anti-Arg2, and anti-VEGF (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-LXRα and anti-ApoE (Abcam, Cambridge, UK); anti-ABCA1 (Novus Biologicals, Littleton CO, USA); anti-phospho-STAT1 (Tyr-701, Ser-727) and total STAT1 (Cell Signaling Technology, Beverly, MA, USA) and anti-α-tubulin (Sigma-Aldrich). DNA polymerase Klenow fragment and dNTPs were obtained from Intron Biotechnology (Seoul, Korea).