Cells were grown in SILAC MEM medium (Thermo Fisher Scientific) lacking lysine and arginine, supplemented with dialysed 10% FBS (Sigma-Aldrich), 200 mg/l light L-Proline (Sigma-Aldrich) and either heavy L-Lysine (13C615N2; 146 mg/l) and L-Arginine (13C615N4; 84 mg/l) (Cambridge Isotope Laboratories) or their light equivalents (Sigma-Aldrich). Cells were passaged with non-enzymatic Gibco Cell Dissociation Buffer (Thermo Fisher Scientific). >95% heavy amino acid incorporation rate was validated by mass-spectrometry.
Light l proline
Manufactured by Merck Group
Light L-Proline is a laboratory reagent used in various biochemical and analytical applications. It is a naturally occurring amino acid that serves as a structural component of proteins. This product provides a consistent and reliable source of L-Proline for research and testing purposes.
Automatically generated - may contain errors
2 protocols using light l proline
1
SILAC Metabolic Labeling Protocol
2
SILAC Metabolic Labeling Protocol
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