Anti p csrc

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-p-cSrc is a laboratory reagent that can detect the phosphorylated form of the cellular Src (c-Src) protein. It is used for research purposes in the study of cell signaling pathways involving c-Src.

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3 protocols using anti p csrc

Protein Expression and Imaging Protocol

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Anti-PDIA1 (BD Bioscience, #610946, 1:1000), PDI Monoclonal Antibody RL90 (#MA3-019, 1:200) p-VEGFR2(1175) (Cell Signaling (CS), #3770, 1:1000), anti-VEGFR2 (CS#2479, 1:1000), anti-p-ERK1/2 (CS#9101, 1:1000), anti-ERK1/2 (CS#9102, 1:1000), anti-p-cSrc (CS#2101 1:1000), anti-cSrc (Santa Cruz # 5266, 1:1000), anti-PTP1B (D-4)(Santa Cruz, #133259, 1:1000), anti-Actin (Santa Cruz, #47778, 1:1000), anti-Rab5 (Santa Cruz #46692, 1:200), anti-Rab7 (B-3) (Santa Cruz# 376362, 1:200), anti CD31 (BD Biosciences# 550274, 1:200), anti-IsolectinB4 (Vector #B-1205, 1:200), anti-Flag (Sigma, #F7425, 1:1000) were used. Secondary antibodies, Goat Anti-Rabbit IgG–HRP conjugate (Bio Rad, #170-6515, 1:2000), Goat Anti-mouse IgG–HRP conjugate (Bio Rad, #170-6516, 1:2000), Alexa Fluor 568 goat anti Rat IgG (Invitrogen, # A-11077, 1:1000), Alexa Fluor 488 goat anti mouse IgG (Invitrogen, # A11001, 1:1000), Alexa Fluor-488-goat anti rabbit IgG (Invitrogen, #A11008), Alexa Fluor-546-goat anti mouse IgG (Invitrogen, #A11003), Alexa Fluor-546-goat anti rabbit IgG (Invitrogen, #A11010), Alexa Fluor-488-goat anti mouse IgG (Invitrogen, #A11001)were used.

Nagarkoti S., Kim Y.M., Ash D., Das A., Vitriol E., Read T.A., Youn S.W., Sudhahar V., McMenamin M., Hou Y., Boatwright H., Caldwell R., Essex D.W., Cho J., Fukai T, & Ushio-Fukai M. (2022). Protein disulfide isomerase A1 as a novel redox sensor in VEGFR2 signaling and angiogenesis. Angiogenesis, 26(1), 77-96.

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Inhibition of EGFR and Inflammatory Signaling

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LPS, TLR4 inhibitor TAK242, c-Src inhibitor PP2, AG1478 (abbreviated AG) were purchased from Sigma Chemical Co. (St. Louis, MO). EGFR small interfering RNA (Si-EGFR), Anti-p-EGFR and anti-EGFR were purchased from Cell Signaling (Danvers, MA). Anti-CD68, anti-p-AKT, anti-AKT, Anti-p-ERK, anti-ERK, anti-IκBα, anti-GAPDH, anti-p-c-Src and anti-TLR4 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). EGFR inhibitor 451, provided by our own lab with HPLC purity > 99%, was identified using ESI-MS and dissolved in dimethyl sulfoxide (DMSO) for in vitro experiments and in 0.5% CMCNa for in vivo experiments.

Shan X., Zhang Y., Chen H., Dong L., Wu B., Xu T., Hu J., Liu Z., Wang W., Wu L., Feng Z, & Liang G. (2017). Inhibition of epidermal growth factor receptor attenuates LPS-induced inflammation and acute lung injury in rats. Oncotarget, 8(16), 26648-26661.

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Comprehensive Signaling Pathway Analysis

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The following reagents and materials were used: anti-GAPDH (Calbiochem), anti-PKC-Pan, anti-pPKC-pan (T514), anti-pAkt (Ser473), anti-Akt, anti-cJUN, anti-phospho cJUN (S73), anti-NF-κB p65, anti-NF-κB phospho-p65 (S536), anti-IκB-alpha, anti-MEK, anti-pMEK (S217/221), anti-PKAc alpha, anti-pPKAc (T197), anti-GSK-3α/β, anti-pGSK-3α/β (S21/9) (Cell Signaling), anti-PP1, anti-c-src, anti-p-c-src (Y419), anti-E1A protein (Santa Cruz Biotechnology), anti-phospho PPP1A (T320), anti-PP2Aα/β (Abcam), and anti-fiber protein (Neomarkers). Electrophoresis reagents were purchased from Bio-rad and trypsin from Promega.

González-Morales A., Zabaleta A., García-Moure M., Alonso M.M., Fernández-Irigoyen J, & Santamaría E. (2019). Oncolytic adenovirus Delta-24-RGD induces a widespread glioma proteotype remodeling during autophagy. Journal of proteomics, 194.

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