Log In Sign up
The largest database of trusted experimental protocols

Facsaria flow cytometric cell sorter

Manufactured by BD
Visit Supplier

The FACSAria flow cytometric cell sorter is a laboratory instrument designed for the analysis and sorting of individual cells. It utilizes flow cytometry technology to detect and differentiate cells based on their physical and fluorescent characteristics.

Automatically generated - may contain errors

Lab products found in correlation

Liberase, by Roche (2 mentions) Dnase 1, by Roche (2 mentions) Mouse dc enrichment kit, by Thermo Fisher Scientific (2 mentions) Cd11c dc macs separation kit, by Miltenyi Biotec (2 mentions)

Spelling variants of this product

FACSAria (4019 citations) FACSAria cell sorter (1038 citations) FACSAria sorter (217 citations) FACSAria flow sorter (15 citations) FACSAria III flow cytometric cell sorter (5 citations)

3 protocols using facsaria flow cytometric cell sorter

1

Isolation and Purification of DC Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymph nodes were isolated and digested with Liberase TM (Roche, Woerden, The Netherlands) in the presence of DNAse I (Roche) at 37 °C after which single cell suspensions were prepared. FACS analysis of the DC subsets was performed on the digested cell suspension according to the indicated gating strategy (Supplementary Figure S1). For DC subset purification, CD11c+ cells were enriched from the digested cell suspensions using a mouse DC enrichment kit (Dynal, Oslo, Norway) or CD11c+ DC MACS separation kit (Miltenyi Biotech). To obtain purified DC subsets, flow-cytometric cell sorting based on the expression of CD45, F4/80, MHCII, CD11c, CD11b and CD103 was performed on a FACSAria flow cytometric cell sorter (BD biosciences) according to the indicated gating strategy.
Veenbergen S., van Berkel L.A., du Pré M.F., He J., Karrich J.J., Costes L.M., Luk F., Simons-Oosterhuis Y., Raatgeep R.H., Cerovic V., Cupedo T., McI Mowat A., Kelsall B.L, & Samsom J.N. (2015). Colonic tolerance develops in the iliac lymph nodes and can be established independent of CD103+ dendritic cells. Mucosal immunology, 9(4), 894-906.
+ Open protocol
+ Expand
2

Isolation and Purification of DC Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymph nodes were isolated and digested with Liberase TM (Roche, Woerden, The Netherlands) in the presence of DNAse I (Roche) at 37 °C after which single cell suspensions were prepared. FACS analysis of the DC subsets was performed on the digested cell suspension according to the indicated gating strategy (Supplementary Figure S1). For DC subset purification, CD11c+ cells were enriched from the digested cell suspensions using a mouse DC enrichment kit (Dynal, Oslo, Norway) or CD11c+ DC MACS separation kit (Miltenyi Biotech). To obtain purified DC subsets, flow-cytometric cell sorting based on the expression of CD45, F4/80, MHCII, CD11c, CD11b and CD103 was performed on a FACSAria flow cytometric cell sorter (BD biosciences) according to the indicated gating strategy.
Veenbergen S., van Berkel L.A., du Pré M.F., He J., Karrich J.J., Costes L.M., Luk F., Simons-Oosterhuis Y., Raatgeep R.H., Cerovic V., Cupedo T., McI Mowat A., Kelsall B.L, & Samsom J.N. (2015). Colonic tolerance develops in the iliac lymph nodes and can be established independent of CD103+ dendritic cells. Mucosal immunology, 9(4), 894-906.
+ Open protocol
+ Expand
3

Directed Differentiation of mESC

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mESC were cultured on 0.1% gelatin-coated culture dish (Sigma) in the absence of leukemia inhibitory factor (LIF). Differentiation was induced by retinoic acid (RA, Sigma-Aldrich). RA was added to the medium at a final concentration of 10-5 M for 72 hr. GFP-positive cells were selected using fluorescence-activated cell sorter (FACS). Cell sorting was carried out with about 6×106 cells of mouse embryonic stem cell by using FACS Aria flow cytometric cell sorter (FACSAria, BD Biosciences). GFP-positive cells were cultured on 0.1% gelatin-coated culture dish in the presence of RA in final concentration of 10-5 M for 30 days to continue differentiation.
Ebrahimzadeh-Vesal R., Shokrgozar M.A., Nayernia K., Teimoori-Toolabi L., Miryounesi M., Nourashrafeddin S., Ranji N, & Modarressi M.H. (2014). A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells. Iranian Journal of Basic Medical Sciences, 17(8), 566-570.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!