Anti rbx1

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-RBX1 is a primary antibody that specifically recognizes the RBX1 protein. RBX1 is a component of the cullin-RING ubiquitin ligase complex that plays a role in the ubiquitination and proteasomal degradation of target proteins.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti rbx1

Western Blot Analysis of Cullin Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total cell extracts were prepared using Pierce IP lysis buffer (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. Equal amounts of proteins were subjected to WB analysis. The antibodies used for WBs were anti-β-Actin (mouse, Sigma, USA), anti-CUL1 (rabbit, Thermo Fisher Scientific, USA), anti-CUL2 (mouse, Santa Cruz Biotechnology, USA), anti-CUL3 (rabbit, Cell Signaling Technology, USA), anti-CUL4A (mouse, Sigma, USA), anti-CUL4B (mouse, Sigma, USA), anti-CUL5 (rabbit, Abcam, USA), anti-CUL7 (rabbit, OriGene, USA), anti-Flag (mouse, Sigma, USA), anti-RBX1 (rabbit, Cell Signaling Technology, USA), anti-DCAF1 (rabbit, Abcam, USA), anti-DCAF4 (rabbit, Sigma, USA), anti-DCAF8 (rabbit, Sigma, USA), anti-DCAF11 (rabbit, Sigma, USA), anti-DCAF15 (rabbit, Sigma, USA), anti-DDB1 (rabbit, Sigma, USA), anti-p21 (rabbit, Sigma, USA), and anti-p27 (mouse, Abcam, USA). Signals from WBs were recorded using a ChemiDoc MP (Bio-Rad, USA). All experiments were performed in triplicate.

Chen Z., Wang K., Hou C., Jiang K., Chen B., Chen J., Lao L., Qian L., Zhong G., Liu Z., Zhang C, & Shen H. (2017). CRL4BDCAF11 E3 ligase targets p21 for degradation to control cell cycle progression in human osteosarcoma cells. Scientific Reports, 7, 1175.

+ Open protocol

+ Expand

Comprehensive Antibody Optimization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All antibodies were used at a 1:1,000 dilution in TBS with 0.1% Tween 20 detergent buffer with 5% non-fat milk for Western blotting. Anti-Myc-Tag antibody (2278), anti-HA antibody (3724), anti-Phospho-Akt Substrate (RXRXXpS*/pT*) antibody (10001), anti-p-AKT (Ser473; 4060), anti- anti-p-p70 S6 Kinase (Thr389; 9234), anti-p-S6 Ribosomal Protein (Ser240/244; 5364), anti-p-4EBP1 (Thr37/46; 2855), pIRF3 antibody (Ser386; 37829), anti-IRF3 antibody (4302), anti-IRF7 antibody (4920), anti- anti-p-TBK1 (Ser172; 5483), anti-TBK1 antibody (51872), anti-STING antibody (13647), anti-c-Myc antibody (18583), anti-RSK1/2/3 antibody (9347), anti-Rictor antibody (9476), anti-Rbx1 (11922), anti-Skp1 antibody (12248), anti-rabbit IgG, HRP-linked antibody (7074), and anti-mouse IgG, HRP-linked antibody (7076) were obtained from Cell Signaling Technology. Anti-cyclin E antibody (sc-198), anti-β-catenin antibody (sc-59737), anti-c-Jun antibody (sc-45), anti-GST antibody (sc-459), anti-Cul1 (sc-11384), and anti-vinculin antibody (sc-25336) were obtained from Santa Cruz Biotechnology. Polyclonal anti-Flag antibody (F-7425), monoclonal anti-Flag antibody (F-3165, clone M2), and anti-α-tubulin antibody (T-5168) were obtained from Sigma-Aldrich. Anti-BUD13 antibody (20163-1-AP) and anti-Fbw7 antibody (28424-1-AP) were obtained from Proteintech.

Chen J., Zhang X., Tan X, & Liu P. (2023). Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function. The Journal of Experimental Medicine, 220(10), e20222056.

+ Open protocol

+ Expand

Immunoblotting of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysis from whole-cell extracts of cultured murine and human cell lines was prepared in diluted RIPA buffer (10× concentration; Cell Signaling 9806). Phosphatase and protease inhibitors were added to the RIPA buffer prior to extraction. Cells were dissociated by mechanical homogenization followed by repeated passaging through successive small gauge needles. Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific 23227). Immunoblotting was performed using standard protocols. The following antibodies were used in this study: anti-β-ACTIN (1:2000; Cell Signaling 4970), anti-GAPDH (1:2000; Santa Cruz Biotechnology sc-32233), anti-NEDD8 (1:1000; Cell Signaling 2745), anti-RBX1 (1:1000; Cell Signaling 11922), anti-INSM1 (1:1000; Santa Cruz Biotechnology sc-271408), anti-MASH1 (ASCL1; 1:1000; BD 24B72D11.1), anti-FOXA2 (1:1000; Cell Signaling 8186), anti-BRN2 (POU3F2; 1:1000; Proteintech 18998-1-AP), anti-NRF2 (1:1000; Cell Signaling 12721), anti-P21 (1:1000; Santa Cruz Biotechnology sc-6246), anti-CSN4 (COPS4; 1:1000; Bethyl Laboratories A300-013A), anti-PARP (1:1000; Cell Signaling 9542), anti-Caspase 7 (1:1000; Cell Signaling 12827), anti-IKKα (1:1000; Cell Signaling 2682), anti-MIOS (1:1000; Bethyl Laboratories A304-699A), and anti-IRAK1 (1:1000; Santa Cruz Biotechnology sc-5288).

Norton J.P., Augert A., Eastwood E., Basom R., Rudin C.M, & MacPherson D. (2021). Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas. Genes & Development, 35(11-12), 870-887.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!