Three mice from each group were anaesthetized by intraperitoneal injection of 1% pentobarbital and tails were intravenously injected with [18F]FDG (Guangzhou HTA Isotope Medical Co. Ltd) (10–11 MBq) at 24, 48 and 72 h after infection with C. albicans (5 × 105 CFU). Forty minutes after the injection, the mice were scanned with a small-animal PET/CT scanner (Inveon) for 20 min. All PET images were reconstructed with the maximum a posteriori (MAP) reconstruction algorithm in Inveon Acquisition Workspace (Siemens Medical Solutions Inc.) and analysed with Inveon Research Workspace 4.2 (Siemens Medical Solutions Inc.) after the coregistration of the PET/CT images. Regions of interest (ROIs) were drawn in the kidneys. The radioactivity density from image analysis is presented as the percent injected dose per gram (% ID/g). After the final imaging time point, the mice were sacrificed. The blood, brain, heart, lungs, liver, spleen, kidneys, stomach, colon and muscle were harvested for ex vivo biodistribution analysis. The radioactivity in each organ was measured with an automatic gamma counter (Perkin-Elmer) after which the organ weights were determined with a balance. The biodistribution is presented as a percent of the injected dose per gram (% ID/g)45 (link),46 (link).
Inveon acquisition workspace
Manufactured by Siemens
Sourced in United States
The Inveon Acquisition Workspace is a software interface designed for the control and data acquisition of Siemens' Inveon preclinical imaging systems. It provides a platform for the management and analysis of imaging data collected from these systems.
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4 protocols using inveon acquisition workspace
1
PET/CT Imaging of Candida Infection in Mice
2
Quantitative PET/CT Imaging Analysis
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All PET images were reconstructed with the maximum a posteriori (MAP) reconstruction algorithm in Inveon Acquisition Workspace (Siemens Medical Solutions Inc., USA) and analyzed with Inveon Research Workspace 4.2 (Siemens Medical Solutions Inc., USA) after the co-registration of PET/CT images. Regions of interest (ROIs) were drawn in the heart chamber for blood, whole brain, and liver. The time-activity curves (TAC) of blood, brain, and liver radioactivities at 0, 4, and 21 h were analyzed with Prism 8 (Graphpad). Blood TACs were fitted with one phase decay. Radioactivity density from image analysis is presented as percent injected dose per cubic centimeter (% ID/cc).
3
Comprehensive Multimodal Image Analysis
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Microscopic image process and ROI analyses were performed using ImageJ, LAS X (Leica) and SlidBook6 (3i). For PET/CT data and image processing, Inveon Acquisition Workspace (Siemens) and Inveon Research Workspace 4.2 (Siemens) was used, respectively. Microsoft Excel (ver. 16.35), and GraphPad Prism 8 for macOS were used for general data and statistical analysis. PyMOL 2.0 (Molecular Graphics System) was used to process the structure of capsid. FlowJo v10.1 (Treestar) was used for data analyses of results from flow cytometry. Gating/sorting strategy is presented at Supplementary Fig. 11 and reporting summary.
4
Multimodal Imaging for Myocardial Infarction
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Inveon Acquisition Workspace was used to reconstruct PET and CT images, and Inveon Research Workspace (both Siemens, Knoxville, TN, USA) was used for co-registration of all PET and CT images and for ROI creation and data collection for both [64Cu]Cu-DOTATATE and [68Ga]Ga-RGD. ROIs were drawn simultaneously on images from the 5th-week scan and the follow-up from either week 7 or 8. The images 2-[18F]-FDG from weeks 4 and 10 guided the outline of the infarct area.
The static 2-[18F]FDG images were analyzed using Corridor4DM version 2017 (Invia LLC, Ann Arbor, MI, USA). The images were reoriented into a short axis, a vertical long axis, and a horizontal long axis. The myocardial contour was automatically outlined and subsequently inspected manually by experienced professionals. The images were excluded for further analyses if the quality was too low for proper automatic recognition. The uptake was compared to a normal database created from other male Sprague–Dawley rats. The infarcted area was automatically calculated as a summed score of metabolic defects in the American Heart Association (AHA) 17-segment model.
The static 2-[18F]FDG images were analyzed using Corridor4DM version 2017 (Invia LLC, Ann Arbor, MI, USA). The images were reoriented into a short axis, a vertical long axis, and a horizontal long axis. The myocardial contour was automatically outlined and subsequently inspected manually by experienced professionals. The images were excluded for further analyses if the quality was too low for proper automatic recognition. The uptake was compared to a normal database created from other male Sprague–Dawley rats. The infarcted area was automatically calculated as a summed score of metabolic defects in the American Heart Association (AHA) 17-segment model.
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