Elite lachrom hplc dad system

Chromatographic analyses were conducted using an Elite Lachrom HPLC-DAD system equipped with a UV detector (Hitachi HighTechnologies Co., Tokyo, Japan). The UV detector at 260 nm and analytical YMC ODS-AM (5 mM, 4.6 × 250 mM) column (Kyoto, Japan) were used for quantification based on the internal standard method. The column temperature was 35 °C, and the mobile phase flow rate was 1 mL/min. The eluent was a gradient of solvent (A) = 0.1% acetic acid in water and solvent (B) = 0.1% acetic acid in acetonitrile. The run time was 35 min, and the gradient was as follows: (A)/(B) = 88/12 (0 min) → (A)/(B) = 78/22 (0–18 min) → (A)/(B) = 72/28 (18–28 min) → (A)/(B) = 62/38 (28–35 min). HPLC analysis is shown in Figure 1.

The HPLC analysis was conducted using an Elite Lachrom HPLC-DAD system equipped with a UV detector (Hitachi HighTechnologies Co., Tokyo, Japan) and a TSK-gel 100 V (4.6 mm × 250 mm, 5 µm; TOSOH, Tokyo, Japan) equipped with a photodiode array detector with a wavelength set at 310 nm. The temperature of the column oven was 25 °C, and the injection volume was 10 μL. The elution was performed at a flow rate of 1.0 mL/min, using acetonitrile (A) and 0.04% trifluoroacetic acid (TFA) (B) as the mobile phase. The solvent gradient was established according to the conditions listed in Table 1. 4-Hydroxybenzyl alcohol is one of the representative GE ingredients, and we confirmed this ingredient, which we expected to play an important role in antithrombotic pharmacological action, through HPLC.